基于Box Behnken响应面法优化口腔模拟环境IgY抑制牙龈卟啉单胞菌增殖

发布时间：2018-07-14 09:05

【摘要】：【目的】利用Box Behnken响应面法研究免疫球蛋白Y(Ig Y)影响牙龈卟啉单胞菌(Porphyromonas gingivalis)的生长繁殖特性,发现口腔模拟环境中P.gingivalis的响应值与Ig Y剂量、初始P.gingivalis数量、培养时间的关系。【方法】以P.gingivalis最终的菌落数为评价指标,通过单因素试验研究培养时间、Ig Y剂量和初始P.gingivalis数量的最佳条件,再依据Box Behnken中心组合原则设计三因素三水平响应面试验进行优化,评价Ig Y的抑制效果。优化得到的试验条件重复3次,以验证模型的准确性。【结果】单因素试验结果表明,以180 mmol/L的Ig Y剂量,2×10~6 CFU/m L的初始P.gingivalis数量,37°C厌氧培养4 h,口腔模拟环境Ig Y对P.gingivalis繁殖的影响达到最佳状态。通过Design Expert 8.0软件对回归方程的分析得到各因素最佳条件为:Ig Y剂量165 mmol/L、初始P.gingivalis数量2×10~6 CFU/m L、培养时间4.5 h。采用优化实验条件,重复3次试验,P.gingivalis最终菌落数是5.92×10~5 CFU/m L,与模型预测值5.85×10~5 CFU/m L相对误差1%,方差分析表明模型P值显著,缺失值P值不显著,理论值与实测值拟合良好。【结论】Ig Y能显著抑制牙龈卟啉单胞菌的增殖,优化后口腔模拟环境中的P.gingivalis最终菌落数显著降低,回归模型能够预测口腔模拟环境中P.gingivalis的增殖。
[Abstract]:[objective] to study the effects of immunoglobulin Y (Ig Y) on the growth and reproduction of Porphyromonas gingivalis by using Box Behnken response surface method, and to find out the response value of P.gingivalis, the dose of Ig Y and the number of initial P.gingivalis in oral simulated environment. [methods] using the final colony number of P.gingivalis as the evaluation index, the optimal conditions of the dose of Ig Y and the initial number of P.gingivalis were studied by single factor experiment. According to Box Behnken center combination principle, three factors and three levels response surface test were designed to optimize the inhibition effect of IgY. The optimized test conditions were repeated three times to verify the accuracy of the model. When the initial P.gingivalis amount of 180 mmol / L Ig Y was 2 脳 10 ~ (6) CFU / mL, the initial P.gingivalis was incubated at 37 掳C for 4 h, the effects of oral simulated environment IGY on P. gingivalis reproduction reached the best condition. Through the analysis of regression equation by Design expert 8.0 software, the optimum conditions were obtained as follows: 1: Ig Y dose 165 mmol / L, initial P.gingivalis quantity 2 脳 10 ~ (6) CFU / m ~ (-1), culture time 4.5 h. The final colony number of P.gingivalis was 5.92 脳 10 ~ (5) CFU / mL, and the relative error was 1 with the predicted value of 5.85 脳 10 ~ (-5) CFU / mL. The variance analysis showed that the model P value was significant, but the missing value P value was not significant. [conclusion] IGY can significantly inhibit the proliferation of porphyromonas gingivalis, and the final colony number of P.gingivalis in the optimized oral simulation environment is significantly reduced. The regression model can predict the proliferation of P.gingivalis in oral simulation environment.
【作者单位】：上海海洋大学食品学院;上海美加净日化有限公司;上海水产品加工与贮藏工程技术研究中心;上海海洋大学海洋科学研究院;
【基金】：国家自然科学基金项目(No.81502955) 上海市进一步加快中医药事业发展三年行动计划(No.ZY3-CCCX-3-3044)~~
【分类号】：R781.41

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