生理性根吸收过程中乳牙牙髓干细胞通过α7 nAChR调节破骨细胞分化能力的研究
发布时间：2018-07-14 09:21
【摘要】：目的:探讨生理性根吸收过程中乳牙牙髓干细胞(DDPSCs)通过α7亚型烟碱型乙酰胆碱受体(α7 nAChR)对破骨分化能力的调节作用。方法:酶消化法和有限稀释法分离培养根吸收不同时期DDPSCs和恒牙牙髓干细胞(DPSCs)。采用实时定量PCR和Western blot,检测各组细胞α7 nAChR、RANKL、OPG的表达及其比值差异,以及阻断α7 nAChR后RANKL、OPG的表达及其比值变化。结果:α7 nAChR表达及RANKL/OPG比值在根吸收中期DDPSCs中明显升高(P0.05)。阻断α7nAChR后,RANKL/OPG明显下降(P0.05)。结论:在乳牙生理性根吸收过程中,α7nAChR通过上调自身表达,参与对RANKL/OPG的调节,影响DDPSCs的破骨分化能力。
[Abstract]:Aim: to investigate the effect of primary dental pulp stem cells (DDPSCs) on osteoclast differentiation through 伪 7 subtype nicotinic acetylcholine receptor (伪 7 nAChR) during physiological root resorption. Methods: DDPSCs and DPSCs were isolated and cultured by enzyme digestion and limited dilution. Real time quantitative PCR and Western blot were used to detect the expression and ratio difference of 伪 7 nAChR RANKL OPG, and the expression and ratio of RANKL1 OPG after blocking 伪 7 nAChR. Results: the expression of 伪 7 nAChR and the ratio of RANKL / OPG were significantly increased in DDPSCs (P0.05). After blocking 伪 7 nAChR, RANKL / OPG decreased significantly (P 0.05). Conclusion: during the process of physiologic root resorption of deciduous teeth, 伪 7nAChR participates in the regulation of RANKL / OPG by upregulating its own expression, thus affecting the osteoclastic differentiation of DDPSCs.
【作者单位】： 军事口腔医学国家重点实验室国家口腔疾病临床医学研究中心陕西省口腔疾病临床医学研究中心第四军医大学口腔医院儿童口腔科;中国人民武装警察部队后勤学院附属医院口腔科;
【基金】：国家自然科学基金(编号:81470743,81500805)
【分类号】：R780.2
[Abstract]:Aim: to investigate the effect of primary dental pulp stem cells (DDPSCs) on osteoclast differentiation through 伪 7 subtype nicotinic acetylcholine receptor (伪 7 nAChR) during physiological root resorption. Methods: DDPSCs and DPSCs were isolated and cultured by enzyme digestion and limited dilution. Real time quantitative PCR and Western blot were used to detect the expression and ratio difference of 伪 7 nAChR RANKL OPG, and the expression and ratio of RANKL1 OPG after blocking 伪 7 nAChR. Results: the expression of 伪 7 nAChR and the ratio of RANKL / OPG were significantly increased in DDPSCs (P0.05). After blocking 伪 7 nAChR, RANKL / OPG decreased significantly (P 0.05). Conclusion: during the process of physiologic root resorption of deciduous teeth, 伪 7nAChR participates in the regulation of RANKL / OPG by upregulating its own expression, thus affecting the osteoclastic differentiation of DDPSCs.
【作者单位】： 军事口腔医学国家重点实验室国家口腔疾病临床医学研究中心陕西省口腔疾病临床医学研究中心第四军医大学口腔医院儿童口腔科;中国人民武装警察部队后勤学院附属医院口腔科;
【基金】：国家自然科学基金(编号:81470743,81500805)
【分类号】：R780.2
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