牙釉质结合肽的体外矿化研究
发布时间：2018-07-14 09:54
【摘要】：本研究首次报道具有牙釉质结合活性的环肽序列(-CMPQVMPMC-)。在反筛试验中,投入等量牙釉质粉末和相同滴度噬菌体,外壳蛋白pⅢ上表达该展示肽的噬菌体回收量明显高于不含展示肽的野生型噬菌体M13。固相法体外合成该环状七肽以模拟展示肽在噬菌体表面的构象,并利用96孔板体外快速凝胶单扩散系统验证其对钙/磷沉积的影响。矿化开始后的24h内,每隔6h记录各样本在405nm的OD值。各样本的相对浊度表示为所占24h末空白组OD值增量(100%)的百分比。结果显示,牙釉质结合肽(EBP)能明显延迟凝胶中的钙磷成核,且延迟效果与添加的浓度相关。在矿化24h末,含结合肽的各组凝胶内浊度明显低于空白组,但高于BSA组。
[Abstract]:In this study, we first reported a cyclic peptide sequence (CMP QVMPMC-) with enamel binding activity. In the reverse screening test, when the same amount of dental enamel powder and the same titer phage was added, the recovery of the displayed peptide on the coat protein p 鈪，
本文编号：2121277
[Abstract]:In this study, we first reported a cyclic peptide sequence (CMP QVMPMC-) with enamel binding activity. In the reverse screening test, when the same amount of dental enamel powder and the same titer phage was added, the recovery of the displayed peptide on the coat protein p 鈪，
本文编号：2121277
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