口腔鳞癌差异CT抗原基因表达谱分析

发布时间：2018-07-24 20:05

【摘要】：研究目的：通过RNA转录组测序技术,检测口腔鳞癌患者肿瘤组织中与正常组织中基因表达差异,并将差异表达基因与已知的CT抗原文库进行匹配,筛选出口腔鳞癌组织差异表达的CT抗原基因,进而通过功能聚类分析,了解这些CT抗原在肿瘤发生发展中的主要影响,为进一步探寻CT抗原作为口腔鳞癌生物标志物提供实验和理论依据。研究方法：1.对口腔鳞癌患者肿瘤组织与正常组织的RNA转录组测序,获得初始数据,过滤之后与参考基因组序列进行匹配,得到匹配的有效测序量,根据此结果确定差异基因。2.检索肿瘤抗原库,筛选出CT抗原,整理CT抗原抗原基因谱,进而将差异基因与CT抗原基因谱进行比对,得到口腔鳞状细胞癌差异表达的CT抗原。3.对口腔鳞状细胞癌差异表达的CT抗原进行功能聚类分析,了解这些CT抗原在肿瘤的发生发展中的主要功能和作用。研究结果：1.送检的口腔鳞癌组织与正常粘膜组中表达差异基因有808个,其中在肿瘤组中表达量高于正常粘膜组的差异基因有470个,表达量低于正常粘膜组的差异基因有338个。2.808个差异基因中有12个差异CT抗原,分别为MAGE家族(CT1.3,CT1.4, CT1.6, CT1.10, CT1.11)、PRAME (CT130)、ARMC3(CT81)、CAGE1(CT95)、 DMRTA1(CT154)、KIFC2(CT139)、LYPD6B(CT116)和TMEFF2(CT120.2)。3.这些差异CT抗原肿瘤的发生发展过程中,可能通过以下几个方面对该进程产生了影响：1、通过与特定蛋白质结合,影响了细胞正常的信号转导功能,进而对细胞的生长发育产生影响；2、影响细胞的分化过程,抑制细胞凋亡并促进细胞的增殖；3、与细胞微管结构相结合,并能结合ATP,对细胞分裂过程中染色体的分离产生影响；4、作为跨膜蛋白或者膜锚定蛋白,其脱落可能对肿瘤细胞转移侵袭产生影响。研究结论：1.在送检的样本中,有12个CT抗原在口腔鳞癌中表达；2.在口腔鳞癌中表达的CT抗原分别为：MAGE家族(CT1.3, CT1.4, CT1.6, CT1.10, CT1.11)、PRAME (CT130)、ARMC3(CT81)、CAGE1(CT95)、 DMRTA1(CT154)、KIFC2(CT139)、LYPD6B(CT116)、 TMEFF2(CT120.2)。这些CT抗原可能成为口腔鳞癌早期诊断、肿瘤预后、复发及转移的检测指标。
[Abstract]:Objective: to detect the difference of gene expression between oral squamous cell carcinoma (OSCC) and normal tissues by RNA transcriptome sequencing, and to match the differentially expressed genes with the known CT antigen library. The differentially expressed CT antigen genes in oral squamous cell carcinoma (OSCC) were screened, and the main effects of CT antigens on tumor development were analyzed by functional cluster analysis. To further explore CT antigen as a biomarker of oral squamous cell carcinoma to provide experimental and theoretical basis. Research method: 1. The RNA transcriptome of tumor tissue and normal tissue of oral squamous cell carcinoma were sequenced, the initial data were obtained, and then matched with the reference genomic sequence, the effective sequencing quantity was obtained. According to the results, the differentially expressed gene. 2. The tumor antigen library was searched, CT antigens were screened, CT antigenic gene profiles were sorted out, and then the differentially expressed CT antigens. 3 were obtained by comparing the differentially expressed CT antigens with CT antigenic gene profiles of oral squamous cell carcinoma (OSCC). The functional cluster analysis of CT antigens differentially expressed in oral squamous cell carcinoma (SCC) was carried out to understand the main function and role of CT antigens in tumorigenesis and development. The result of the study was: 1. There were 808 differentially expressed genes in oral squamous cell carcinoma (OSCC) and normal mucosa, of which 470 were higher in tumor group than in normal mucosa group. There were 338 differentially expressed genes in the normal mucosa group. There were 12 differentially expressed CT antigens in the 2.808 differentially expressed genes, namely, MAGE family (CT1.3nCT1.4, CT1.6, CT1.10, CT1.11), PRAME (CT130) ARMC3 (CT81) CAGE1 (CT95), DMRTA1 (CT154) KIFC2 (CT139) LYPD6B (CT116) and TMEFF2 (CT120.2) .3. These differential CT antigenic tumors may have an effect on this process in the following ways: 1, by binding to specific proteins, affecting the normal signal transduction function of the cell. Furthermore, it can affect cell growth and development, affect cell differentiation, inhibit cell apoptosis and promote cell proliferation, bind to cell microtubule structure and bind to ATP, and affect chromosome separation during cell division. 4. As a transmembrane protein or a membrane anchoring protein, its exfoliation may have an effect on the metastasis and invasion of tumor cells. Conclusion: 1. Among the samples examined, 12 CT antigens were expressed in oral squamous cell carcinoma (OSCC). The CT antigens expressed in oral squamous cell carcinoma were as follows: mage family (CT1.3, CT1.4, CT1.6, CT1.10, CT1.11), PRAME (CT130) ARMC3 (CT81) CAGE1 (CT95), DMRTA1 (CT154) KIFC2 (CT139) LYPD6B (CT116), TMEFF2 (CT120.2). These CT antigens may be used as early diagnosis, prognosis, recurrence and metastasis of oral squamous cell carcinoma.
【学位授予单位】：南京大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.8

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