高糖环境通过抑制CXCR-4影响大鼠下颌骨骨髓基质细胞迁移

发布时间：2018-07-31 12:02

【摘要】：目的:探讨高糖环境通过抑制CXCR-4对大鼠下颌骨骨髓基质细胞迁移的影响。方法:从Wistar大鼠下颌骨中分离、培养骨髓基质细胞,利用Transwell实验筛选在生理糖浓度(5.5 mmol/L)下的SDF-1最适迁移浓度,检测不同糖浓度(5.5、16.5 mmol/L)条件下SDF-1、AMD3100对细胞迁移能力的影响。利用Western免疫印迹检测不同糖浓度(5.5、16.5 mmol/L)条件下CXCR-4的表达,实时定量PCR检测CXCR-4、MMP-2的m RNA表达。采用SPSS 11.0软件包对数据进行统计学分析。结果:Transwell检测显示,生理糖浓度下,骨髓基质细胞的迁移能力对趋化因子SDF-1呈浓度依赖性;在生理糖浓度下,趋化因子SDF-1的最适浓度为100 ng/m L。高糖环境可抑制骨髓基质细胞的迁移能力。在不同糖浓度下,SDF-1均可明显增强细胞的迁移能力。加入AMD3100后,这种增强趋势受到明显抑制。高糖环境抑制CXCR-4蛋白以及CXCR-4和MMP-2 m RNA表达。结论:高糖环境可以通过抑制CXCR-4的表达来影响大鼠下颌骨骨髓基质细胞在SDF-1/CXCR-4生物轴调控下的定向迁移能力。
[Abstract]:Aim: to investigate the effect of high glucose environment on the migration of mandibular bone marrow stromal cells by inhibiting CXCR-4. Methods: bone marrow stromal cells were isolated from the mandible of Wistar rats and cultured. The optimal migration concentration of SDF-1 under physiological glucose concentration (5.5 mmol/L) was screened by Transwell assay, and the effect of SDF-1AMD3100 on cell migration ability under different glucose concentration (5.5 mmol/L) was detected. Western Western blot was used to detect the expression of CXCR-4 at different glucose concentrations (5.5g / 16.5 mmol/L), and real-time quantitative PCR was used to detect the expression of m RNA in CXCR-4 MMP-2. The data were analyzed by SPSS 11.0 software package. Results the migration ability of bone marrow stromal cells was concentration-dependent on chemokine SDF-1 under physiological glucose concentration, and the optimal concentration of chemokine SDF-1 was 100 ng/m / L. High glucose environment can inhibit the migration ability of bone marrow stromal cells. SDF-1 could significantly enhance the migration ability of cells at different glucose concentrations. After joining AMD3100, this enhancement trend is obviously inhibited. High glucose environment inhibited the expression of CXCR-4 protein and CXCR-4 and MMP-2 m RNA. Conclusion: high glucose environment can inhibit the expression of CXCR-4 to influence the directional migration of rat mandibular bone marrow stromal cells under the control of SDF-1/CXCR-4 biological axis.
【作者单位】：解放军总医院口腔医学研究所;
【基金】：国家自然科学基金(81271180,31200741)~~
【分类号】：R783.6;R587.1

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