氟环境下变形链球菌耐氟菌株ciaH、eno和pykF基因的差异表达及其意义
发布时间：2018-07-31 12:55
【摘要】：目的:检测变形链球菌耐氟菌株在氟环境下ciaH、eno和pykF基因表达的差异,探讨ciaH、eno和pykF基因与变形链球菌氟抗性的关系。方法:变形链球菌亲代菌株及其耐氟菌株,分为亲代组(变形链球菌亲代菌株生长于无氟BHI培养基中)、耐氟组(耐氟菌株生长于无氟BHI培养基中)和含氟组(耐氟菌株生长于含氟量为1g·L~(-1)的BHI培养基中)。分别选取3组处于生长对数期(11h)和稳定期(20h)的菌株,采用RT-PCR法检测ciaH、eno和pykF mRNA表达水平。结果:与耐氟组比较,含氟组的耐氟菌株ciaH、eno和pykF mRNA表达水平在对数期和稳定期均明显升高(P0.05或P0.01);与亲代组比较,耐氟组的耐氟菌株eno和pykF mRNA表达水平在对数期和稳定期均明显降低(P0.01),ciaH mRNA表达水平在对数期差异无统计学意义(P0.05),在稳定期明显升高(P0.01)。结论:氟能提高耐氟菌株ciaH、eno和pykF基因的表达,该组基因与耐氟菌株氟抗性的产生有关联。
[Abstract]:Objective: to detect the difference of the expression of Cia Hneno and pykF genes between Streptococcus mutans and Streptococcus mutans in fluorine environment, and to explore the relationship between Cia Hneno and pykF genes and the fluorine resistance of Streptococcus mutans. Methods: Streptococcus mutans parents and fluorine tolerant strains were used. It was divided into three groups: parent group (Streptococcus mutans grew in BHI medium without fluorine), fluorine tolerant group (fluorine tolerant strain grew in BHI medium without fluoride) and fluorine group (fluoride tolerant strain grew in BHI medium with fluorine content of 1 g L ~ (-1). Three groups of strains in logarithmic phase (11 h) and stable stage (20 h) were selected to detect the expression of Cia Hmeno and pykF mRNA by RT-PCR method. Results: compared with the fluorine-tolerant group, the expression levels of ciaHeno and pykF mRNA in fluorine-tolerant strain were significantly increased in logarithmic phase and stable period (P0.05 or P0.01), and compared with parent group, the expression level of Cia Hneno and pykF mRNA in fluorine-tolerant group were significantly higher than those in control group (P0.05 or P0.01). The expression levels of eno and pykF mRNA in fluorine-tolerant strains were significantly decreased in logarithmic phase and stable phase (P0.01). There was no significant difference in the expression level of ciaH mRNA in logarithmic phase (P0.05), but significantly increased in stable phase (P0.01). Conclusion: fluorine can enhance the expression of Cia Hneno and pykF genes in fluorine tolerant strains, which is related to the production of fluorine resistance in fluorine tolerant strains.
【作者单位】： 吉林大学口腔医院牙体牙髓病科;
【基金】：吉林省科技厅自然科学基金资助课题(20160101003JC)
【分类号】：R780.2
，
本文编号：2155646
[Abstract]:Objective: to detect the difference of the expression of Cia Hneno and pykF genes between Streptococcus mutans and Streptococcus mutans in fluorine environment, and to explore the relationship between Cia Hneno and pykF genes and the fluorine resistance of Streptococcus mutans. Methods: Streptococcus mutans parents and fluorine tolerant strains were used. It was divided into three groups: parent group (Streptococcus mutans grew in BHI medium without fluorine), fluorine tolerant group (fluorine tolerant strain grew in BHI medium without fluoride) and fluorine group (fluoride tolerant strain grew in BHI medium with fluorine content of 1 g L ~ (-1). Three groups of strains in logarithmic phase (11 h) and stable stage (20 h) were selected to detect the expression of Cia Hmeno and pykF mRNA by RT-PCR method. Results: compared with the fluorine-tolerant group, the expression levels of ciaHeno and pykF mRNA in fluorine-tolerant strain were significantly increased in logarithmic phase and stable period (P0.05 or P0.01), and compared with parent group, the expression level of Cia Hneno and pykF mRNA in fluorine-tolerant group were significantly higher than those in control group (P0.05 or P0.01). The expression levels of eno and pykF mRNA in fluorine-tolerant strains were significantly decreased in logarithmic phase and stable phase (P0.01). There was no significant difference in the expression level of ciaH mRNA in logarithmic phase (P0.05), but significantly increased in stable phase (P0.01). Conclusion: fluorine can enhance the expression of Cia Hneno and pykF genes in fluorine tolerant strains, which is related to the production of fluorine resistance in fluorine tolerant strains.
【作者单位】： 吉林大学口腔医院牙体牙髓病科;
【基金】：吉林省科技厅自然科学基金资助课题(20160101003JC)
【分类号】：R780.2
，
本文编号：2155646
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