载多西紫杉醇脂质微泡联合超声靶向微泡破裂对兔VX2舌癌颈部淋巴转移瘤作用的初步研究

发布时间：2018-07-31 14:28

【摘要】：目的:初步探讨载多西紫杉醇脂质微泡(docetaxel-loaded lipid microbubbles,DLLM)联合超声靶向微泡破裂(ultrasound targeted microbubbles destruction,UTMD)对兔VX2舌癌颈部淋巴转移瘤的治疗作用。方法:成功建立兔VX2舌癌颈部淋巴转移瘤动物模型30只,随机分为A组、B组、C组、D组、E组、F组(n=5):A组为单纯药物组、B组为单纯载药微泡组、C组为药物+超声组、D组为单纯微泡+超声组、E组为载药微泡+超声组、F组为对照组。治疗前后用超声测量各组动物颈部淋巴转移瘤大小,计算瘤体体积和抑瘤率;比较各组动物颈部淋巴转移瘤组织中CD34、微血管密度(microvessel density,MVD)及血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达。结果:E组实验动物颈部淋巴转移瘤的抑瘤率高于其他组(P0.05),与其他各组相比差异有统计学意义;E组实验动物颈部淋巴转移瘤中CD34表达水平最低,MVD、VEGF表达水平显著低于其他各组(P0.01)与其他各组相比差异有统计学意义。结论:DLLM联合UTMD不仅能在宏观表现上抑制兔VX2舌癌颈部淋巴转移瘤生长,更能一定程度上从分子生物学水平明显抑制兔VX2舌癌颈部淋巴转移瘤的转移、生长。
[Abstract]:Objective: to investigate the therapeutic effect of docetaxel-loaded lipid microbubbles combined with ultrasound targeted (ultrasound targeted microbubbles destruction (UTMD) on cervical lymphatic metastasis of VX2 tongue cancer in rabbits. Methods: 30 rabbit models of cervical lymphatic metastasis of VX2 tongue carcinoma were established successfully. They were randomly divided into group A, group B, group C, group E, group F (nn 5): group A, group B, drug carrying microbubbles, group B, group C, drug carrying microbubbles, group C, group D, simple ultrasound group, group E, group F, the control group. Before and after treatment, the size of cervical lymphatic metastasis was measured, the tumor volume and tumor inhibition rate were calculated, and the expression of CD34, microvessel density and (vascular endothelial growth (vascular endothelial growth were compared before and after treatment. Results the inhibitory rate of cervical lymphatic metastasis in group E was higher than that in other groups (P0.05). There was a significant difference between group E and other groups in the expression of CD34 in cervical lymphatic metastasis. The expression of CD34 in group E was significantly lower than that in group E (P0.05). The other groups (P0.01) had statistical significance compared with other groups. ConclusionDLLM combined with UTMD can not only inhibit the growth of cervical lymphatic metastasis of VX2 in rabbit tongue cancer, but also inhibit the metastasis and growth of cervical lymphatic metastasis of rabbit VX2 carcinoma of tongue in a certain degree from the molecular biological level.
【作者单位】：重庆医科大学附属口腔颌面外科口腔医院口腔疾病与生物医学重庆市重点实验室;重庆医科大学附属第二医院口腔科;重庆医科大学超声影像学研究所;
【基金】：重庆市医学科技计划资助项目(编号:2011-1-060)
【分类号】：R739.86

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