三氧化二砷与顺铂合用抗人口腔癌细胞株HSQ-89的实验研究

发布时间：2018-08-12 07:33

【摘要】：目的:探讨三氧化二砷能否增强顺铂对口腔癌细胞的抗瘤活性。方法:以人口腔癌细胞株HSQ-89为研究对象,在经顺铂处理过的肿瘤细胞中加入不同浓度的三氧化二砷,用MTT法测定计算肿瘤细胞的抑制率。结果:在加入顺铂15μg/mL的口腔癌细胞珠HSQ-89中加入不同浓度的三氧化二砷0、2.5、5、7.5、10、12.5μmol/mL,肿瘤细胞的抑制率分别为26.9%、67.5%、73.0%、88.5%、90.4%、98.7%;三氧化二砷与顺铂联用,可以提高对HSQ-89细胞的抑制率,并呈剂量依赖性增加。结论:三氧化二砷与顺铂联用,对口腔癌细胞的生长抑制具有协同效应。
[Abstract]:Objective: to investigate whether arsenic trioxide can enhance the anti-tumor activity of cisplatin on oral cancer cells. Methods: the human oral cancer cell line HSQ-89 was studied. Different concentrations of arsenic trioxide were added to the tumor cells treated with cisplatin. The inhibition rate of tumor cells was measured by MTT method. Results: different concentrations of arsenic trioxide (as _ 2O _ 3) were added to oral cancer cell bead HSQ-89 with cisplatin at 15 渭 mol 路mL ~ (-1). The inhibition rates of tumor cells were 26.97.50 渭 mol 路mL ~ (-1) and 77.50 渭 mol 路mL ~ (-1), respectively, and the inhibition rate of HSQ-89 cells was increased in a dose-dependent manner by the combination of arsenic trioxide and cisplatin. Conclusion: arsenic trioxide combined with cisplatin has synergistic effect on the growth inhibition of oral cancer cells.
【作者单位】： 广东省医学科学院 广东省人民医院口腔颌面外科;
【基金】：广东省科技计划项目(编号:20088030301036,20078031514005) 广东省中医药管理局科研项目(编号:20121260)
【分类号】：R739.85
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