慢性牙周炎合并血管钙化大鼠实验模型的建立

发布时间：2018-08-13 08:07

【摘要】：目的:建立一种牙周炎合并血管钙化复合动物模型。方法:20只成年Wistar雄性大鼠随机分为血管钙化+慢性牙周炎组(VDN+CP)和正常对照组(C)(n=10);VDN+CP组采用丝线局部结扎、牙周混合致病菌感染合并维生素D3肌注和尼古丁灌胃的方法建立VDN+CP模型,建模第8周检测各牙周临床指标,然后处死各大鼠,取心脏称重并测量心重指数;取胸主动脉制备石蜡切片,HE染色观察血管改变;取含上颌磨牙的上颌骨,CBCT扫描观察牙槽骨吸收情况。结果:VDN+CP组牙周炎症明显,牙周探诊深度(probing depth,PD)、龈沟出血指数(sulcular bleeding index,SBI)、牙槽骨丧失值以及心重指数均明显高于C组(P0.05);血管病理学观察显示:VDN+CP组胸主动脉形成血管钙化病变,C组未见明显异常改变。结论:通过维生素D3肌注加尼古丁灌胃联合丝线结扎和细菌接种法初步成功建立了慢性牙周炎合并血管钙化动物模型。
[Abstract]:Objective: to establish an animal model of periodontitis combined with vascular calcification. Methods Twenty adult male Wistar rats were randomly divided into two groups: vascular calcified chronic periodontitis group (VDN CP) and normal control group (C) (nong10). The VDN CP model was established by mixed periodontal pathogenic bacteria infection with vitamin D3 intramuscular injection and nicotine administration. The clinical indexes of each periodontal week were measured at the 8th week of modeling. Then the rats were killed, the heart was weighed and the cardiac weight index was measured. The changes of blood vessels were observed by HE staining and the alveolar bone resorption was observed by CBCT scanning of maxillary molars. Results the periodontitis in the VDN CP group was obvious. The periodontal probing depth (probing PD), gingival sulcus bleeding index (sulcular bleeding), alveolar bone loss and cardiac weight index were significantly higher than those in group C (P0.05). Conclusion: the animal model of chronic periodontitis with vascular calcification was established by intramuscular administration of vitamin D _ 3 plus nicotine, ligation of filaments and bacterial inoculation.
【作者单位】：青岛大学医学院附属医院山东省教育厅口腔临床医学重点实验室;
【基金】：国家自然科学基金资助项目(81100755) 青岛市科技局课题[12-1-4-2(18)-jch]
【分类号】：R781.42

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