血管内皮细胞生长因子与碱性成纤维细胞生长因子联合应用对鼠牙周膜成纤维细胞增殖与碱性磷酸酶活性的影响

发布时间：2018-08-22 16:45

【摘要】：背景:碱性成纤维细胞生长因子可以提高成纤维细胞的增殖及胶原的沉积能力,血管内皮细胞生长因子可改善病变组织的血流灌注和新陈代谢水平,且两者在适当的浓度、时间作用下,均可增强碱性磷酸酶的活性。目的:观察血管内皮细胞生长因子与碱性成纤维细胞生长因子联合应用对鼠牙周膜成纤维细胞增殖与碱性磷酸酶活性的影响。方法:(1)体外培养大鼠牙周膜成纤维细胞并鉴定其胚胎来源,第4代细胞用于实验;(2)检测不同浓度血管内皮细胞生长因子、碱性成纤维细胞生长因子对大鼠牙周膜成纤维细胞增殖的影响,并从中确定显效浓度及最大效应浓度;(3)将细胞分为5组,分别为A组:对照组,为含体积分数2%胎牛血清的DMEM;B组:血管内皮细胞生长因子最大效应浓度组;C组:碱性成纤维细胞生长因子最大效应浓度组;D组:血管内皮细胞生长因子显效浓度与碱性成纤维细胞生长因子显效浓度联合应用;E组:血管内皮细胞生长因子最大效应浓度与碱性成纤维细胞生长因子最大效应浓度联合应用。于3,7,14 d后,测定5组细胞的碱性磷酸酶活性。结果与结论:(1)培养的大鼠牙周膜成纤维细胞生长状态良好,来源于中胚层;(2)随着血管内皮细胞生长因子、碱性成纤维细胞生长因子质量浓度的增加,大鼠牙周膜成纤维细胞增殖能力也随之提高(P0.01)。血管内皮细胞生长因子的显效浓度为10μg/L,最大效应浓度为100μg/L;碱性成纤维细胞生长因子的显效浓度为0.1μg/L,最大效应浓度为10μg/L;(3)当血管内皮细胞生长因子、碱性成纤维细胞生长因子联合作用于大鼠牙周膜成纤维细胞时,实验各组A值均高于对照组。(4)在应用3 d和7 d时,但D组A值显著低于E组(P0.05),14 d时2组差异无显著性意义。(5)结果说明,在一定作用时间内,最大效应浓度联合应用对碱性磷酸酶的活性能起到明显的协同作用,但超过某一时间,显效浓度同最大效应浓度对于牙周膜成纤维细胞碱性磷酸酶活性没有差异。这可能与两因子的作用时效及牙周膜细胞的受体有关。
[Abstract]:Background: basic fibroblast growth factor can improve fibroblast proliferation and collagen deposition. Vascular endothelial growth factor can improve the blood flow perfusion and metabolism of pathological tissue at appropriate concentration. The activity of alkaline phosphatase could be enhanced under the action of time. Aim: to observe the effects of vascular endothelial growth factor (VEGF) combined with basic fibroblast growth factor (bFGF) on proliferation and alkaline phosphatase activity of rat periodontal ligament fibroblasts. Methods: (1) Rat periodontal ligament fibroblasts were cultured in vitro and their embryonic origin was identified. Effects of basic fibroblast growth factor on proliferation of periodontal ligament fibroblasts in rats and determination of the apparent and maximum effective concentrations. (3) the cells were divided into five groups: group A: control group. Group B: Vascular endothelial growth factor maximum effect concentration group C: basic fibroblast growth factor maximum effect concentration group D group: vascular endothelial growth factor concentration Group E: the maximum effect concentration of vascular endothelial growth factor and the maximum effect concentration of basic fibroblast growth factor. The alkaline phosphatase activity of 5 groups of cells was measured after 14 days. Results and conclusion: (1) the cultured rat periodontal ligament fibroblasts grew well and originated from mesoderm. (2) with the increase of vascular endothelial growth factor, the concentration of basic fibroblast growth factor increased. The proliferation ability of rat periodontal ligament fibroblasts was also increased (P0.01). The apparent concentration of vascular endothelial growth factor is 10 渭 g / L, the maximum effective concentration is 100 渭 g / L; the apparent concentration of basic fibroblast growth factor is 0.1 渭 g / L, and the maximum effective concentration is 10 渭 g / L; (3) when vascular endothelial growth factor, When combined with basic fibroblast growth factor (bFGF) on periodontal ligament fibroblasts of rats, the A value of each group was higher than that of the control group. (4) after 3 and 7 days of treatment, the A value of each group was higher than that of the control group. However, the value of A in group D was significantly lower than that in group E (P0.05) for 14 days. (5) the results showed that the combined use of maximum effect concentration could play an obvious synergistic effect on the activity of alkaline phosphatase in a certain time, but beyond a certain time. There was no difference between the apparent concentration and the maximum effect concentration on alkaline phosphatase activity of periodontal ligament fibroblasts. This may be related to the aging of two factors and the receptor of periodontal ligament cells.
【作者单位】：沈阳市第六人民医院口腔科;锦州医科大学附属口腔医院修复科;
【基金】：辽宁省科技厅自然科学基金优秀人才培育项目(2014022003) 辽宁省大学生创新训练项目(201510160000045)~~
【分类号】：R781.4

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