白藜芦醇对脱矿牙本质基质影响的相关性研究

发布时间：2018-08-22 19:57

【摘要】：目的:探讨白藜芦醇对基质金属蛋白酶-2活性及耐脱矿力的影响,以期为白藜芦醇的临床应用提供实验依据。方法:设置实验组白藜芦醇的浓度梯度:12.5μmol/L、25μmol/L、50μmol/L、100μmol/L、200μmol/L、400μmol/L,阳性对照组0.2%氯己定,阴性对照组去离子水。每组取10μL与提取出来的牙源性mmp-2 30μL混合孵育30min,用体液基质金属蛋白酶-2活性比色法定量检测各组混合20 min后的酶活性,每组重复5次。牙本质片经pH循环后,用激光共聚焦电子显微镜3D图像观察牙本质脱矿深度。结果:实验组白藜芦醇浓度在50μmol/L以上时有明显抑制作用,且在100μmol/L、200μmol/L、400μmol/L时的酶活性抑制率与阳性对照组间无显著性差异。激光共聚焦电子显微镜3D图像显示100μmol/L Res的染色深度明显低于阴性对照组,统计学分析结果显示100μmol/L Res的平均脱矿深度显著低于阴性对照组(P0.05),与阳性对照组间并无显著性差异。结论:白藜芦醇可抑制基质金属蛋白酶-2活性及牙本质脱矿。
[Abstract]:Objective: to investigate the effect of resveratrol on matrix metalloproteinase-2 activity and demineralization resistance in order to provide experimental evidence for clinical application of resveratrol. Methods: the concentration gradient of resveratrol in the experimental group was set at 25 渭 mol / L ~ (25 渭 mol / L). The concentration gradient of resveratrol was 50 渭 mol / L ~ 100 渭 mol / L ~ (100) 渭 mol / L ~ 200 渭 mol 路L ~ (-1), 0.2% chlorhexidine in the positive control group, and (0.2%) chlorhexidine in the negative control group. 10 渭 L of each group was incubated with the extracted odontogenic mmp-2 30 渭 L for 30 minutes. The enzyme activity of each group was measured by colorimetric assay of matrix metalloproteinase-2 (MMP-2) activity after 20 min mixing and repeated for 5 times in each group. The depth of dentin demineralization was observed by laser confocal electron microscope 3D images after pH cycling. Results: resveratrol concentration in the experimental group was more than 50 渭 mol/L, and the inhibition rate of the enzyme activity at 100 渭 mol / L ~ 200 渭 mol / L ~ 400 渭 mol/L was not significantly different from that of the positive control group. Laser confocal electron microscope 3D images showed that the staining depth of 100 渭 mol/L Res was significantly lower than that of the negative control group, and the statistical analysis showed that the average demineralization depth of 100 渭 mol/L Res was significantly lower than that of the negative control group (P0.05), and there was no significant difference between the 100 渭 mol/L Res and the positive control group. Conclusion: resveratrol can inhibit matrix metalloproteinase-2 activity and dentin demineralization.
【作者单位】：佳木斯大学附属第二医院口腔医院牙体牙髓病二科;苏州市广济医院;
【基金】：佳木斯大学科学技术校级面上项目(编号:13Z1201558),佳木斯大学研究生科技创新项目(编号:LM2015_060)
【分类号】：R78

【相似文献】