IGFBP-3在唾液腺多形性腺瘤中的表达及意义

发布时间：2018-08-27 07:57

【摘要】：目的:研究胰岛素样生长因子结合蛋白3(IGFBP-3)在唾液腺多形性腺瘤(SPA)中的表达。方法:采用Western blot检测IGFBP-3在40例SPA、40例正常腺体组织及10例恶性唾液腺肿瘤组织中的表达。采用实时荧光定量PCR(qRT-PCR)检测IGFBP-3 mRNA在50例SPA组织、50正常腺体组织及10例唾液腺恶性肿瘤中的表达。结果:IGFBP-3蛋白在唾液腺正常组织(N组:8.54±3.95)的表达(A值)显著高于多形性腺瘤组(PA组:4.78±2.07)及恶性肿瘤组(CA组:3.63±2.27)(P0.05)。而PA组与CA组之间则不存在显著差异(P0.05)。IGFBP-3 mRNA在SPA组织与恶性肿瘤组织中的表达均低于正常组织(PA/N组:0.654±0.387,CA/N组:0.452±0.229),PA组与CA组不存在显著差异(P0.05);不同包膜浸润程度的多形性腺瘤组织中相对含量的差异有统计学意义(P0.05),在年龄、性别及有无复发上无明显差异。结论:IGFBP-3在多形性腺瘤中的低表达可能减低了拮抗IGF-1R的作用,引起肿瘤细胞的增殖,促进肿瘤形成。
[Abstract]:Objective: to investigate the expression of insulin-like growth factor binding protein 3 (IGFBP-3) in (SPA) of salivary gland pleomorphic adenoma. Methods: Western blot was used to detect the expression of IGFBP-3 in 40 cases of SPA,40 normal gland and 10 cases of malignant salivary gland tumor. Real-time quantitative PCR (qRT-PCR) was used to detect the expression of IGFBP-3 mRNA in 50 normal glandular tissues of SPA and 10 malignant tumors of salivary gland. Results the expression of 1% IGFBP-3 protein in normal salivary gland (group N: 8.54 卤3.95) was significantly higher than that in pleomorphic adenoma group (PA group: 4.78 卤2.07) and malignant tumor group (CA group: 3.63 卤2.27) (P0.05). However, there was no significant difference between PA group and CA group (P0.05). The expression of IGFBP-3 mRNA in SPA and malignant tumor tissues was lower than that in normal tissue (PA/N group: 0.654 卤0.387 卤0.387%: 0.452 卤0.229). There was no significant difference between PA group and CA group (P0.05), and there was no significant difference between PA group and CA group (P0.05). The difference of relative content in tumor tissue was statistically significant (P0.05). There was no significant difference in sex and recurrence. Conclusion the low expression of WIGFBP-3 in pleomorphic adenomas may reduce the antagonistic effect of IGF-1R, induce the proliferation of tumor cells and promote tumor formation.
【作者单位】：贵州医科大学附属医院口腔颌面外科;
【基金】：贵州省科技厅社会发展项目(编号:黔科合[2013]3012号)
【分类号】：R739.87

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