EMMPRIN在小鼠下颌第一磨牙形态发生及硬组织形成中的功能性作用

发布时间：2018-08-30 12:52

【摘要】：目的研究小鼠下颌第一磨牙中后期牙胚发育过程中细胞外基质金属蛋白酶诱导因子(EMMPRIN)的表达情况及其对牙齿细胞外基质分泌和矿化的影响。方法采用免疫组织化学方法检测EMMPRIN在牙胚发育各个阶段中的表达定位;应用体外牙胚器官培养法以及抗体阻断的方法观察EMMPRIN功能性缺失对于牙胚形态发生的影响;采用Real-Time PCR技术检测EMMPRIN相关基因的表达变化。结果 EMMPRIN蛋白特异性地表达于牙胚的帽状期、钟状期以及其后的分泌期;阻断体外培养牙胚中EMMPRIN的蛋白活性可导致牙釉质形成障碍,同时下调牙胚培养物中EMMPRIN相关基因MMP-9、MMP-13、MMP-20、ALPL、ameloblastin、amelogenin和enamelin mRNA的表达。结论 EMMPRIN的表达贯穿于牙胚发生发育的多个阶段;EMMPRIN可能通过调控金属基质蛋白酶和(或)相关釉质基质蛋白而在成釉细胞的分化及釉质基质的分泌、矿化过程中发挥关键作用。
[Abstract]:Objective to study the expression of extracellular matrix metalloproteinase-inducible factor (EMMPRIN) and its effect on the secretion and mineralization of extracellular matrix during the middle and late development of mouse first molar. Methods Immunohistochemical method was used to detect the expression of EMMPRIN in all stages of tooth germ development, and the effects of EMMPRIN functional deficiency on the morphogenesis of tooth germ were observed by means of in vitro tooth germ organ culture and antibody blocking. Real-Time PCR technique was used to detect the expression of EMMPRIN related genes. Results EMMPRIN protein was specifically expressed in the cap, bell and subsequent secretory phases of tooth germs, and blocking the activity of EMMPRIN protein in vitro resulted in the formation of enamel. At the same time, the expression of EMMPRIN related genes MMP-9,MMP-13,MMP-20,ALPL,ameloblastin,amelogenin and enamelin mRNA in tooth germ culture was down-regulated. Conclusion the expression of EMMPRIN may play a key role in the differentiation of ameloblasts and the secretion of enamel matrix through the regulation of metalloproteinases and / or related enamel matrix proteins.
【作者单位】：福建医科大学附属第一医院口腔颌面外科;上海交通大学医学院附属第九人民医院口腔修复科;
【基金】：上海市重点学科建设项目(S30206) 上海市重点学科(特色学科)建设项目(T0202) 国家自然科学基金(30900848) 福建省卫生厅青年科研基金(2010-1-16)~~
【分类号】：R78

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