低氧对人牙周膜干细胞骨向分化的影响

发布时间：2018-09-03 18:03

【摘要】：目的探讨低氧对人牙周膜干细胞(PDLSCs)骨向分化的影响。方法采用组织块法体外分离培养PDLSCs,分别在常氧和低氧条件下培养PDLSCs,低氧组在2%O2浓度下培养6、12、24、48 h后检测其碱性磷酸酶(ALP)活性,并通过荧光定量PCR检测低氧培养的PDLSCs中骨钙素(OCN)、低氧诱导因子1α(Hif-1α)、碱性磷酸酶(ALP)以及成骨相关基因核心结合因子(Runx-2)等基因的表达变化;通过Western blot法检测低氧培养的PDLSCs中Runx-2、Hif-1α等蛋白的表达变化。结果低氧组的PDLSCs的ALP水平高于常氧组,但低氧48 h开始抑制ALP水平,荧光定量PCR和Western blot结果显示低氧培养48 h内的PDLSCs的成骨能力高于常氧组,但低氧培养48 h则抑制其成骨能力。结论48 h内低氧可显著增强PDLSCs骨向分化作用,48 h则开始抑制其成骨能力。
[Abstract]:Objective to investigate the effect of hypoxia on bone differentiation of human periodontal ligament stem cells (PDLSCs). Methods PDLSCs, was isolated and cultured in vitro by tissue mass method. The (ALP) activity of alkaline phosphatase (ALP) was detected in PDLSCs, hypoxia group cultured at 2%O2 concentration for 48 h after being cultured in normoxic and hypoxic conditions. The expression of osteocalcin (OCN), hypoxia inducible factor 1 伪 (Hif-1 伪), alkaline phosphatase (ALP) and osteoblast-associated gene core binding factor (Runx-2) in hypoxic PDLSCs were detected by fluorescence quantitative PCR. The expression of Runx-2,Hif-1 伪 and other proteins in PDLSCs cultured with hypoxia was detected by Western blot method. Results the ALP level of PDLSCs in hypoxic group was higher than that in normoxic group, but the ALP level was inhibited at 48 h after hypoxia. The results of fluorescence quantitative PCR and Western blot showed that the osteogenic ability of PDLSCs in hypoxia group was higher than that in normoxic group within 48 h. But hypoxia culture for 48 h inhibited its osteogenic ability. Conclusion hypoxia can significantly enhance the osteogenic ability of PDLSCs during 48 h.
【作者单位】：安徽医科大学口腔医学院安徽医科大学附属口腔医院安徽省口腔疾病研究中心实验室;
【基金】：国家自然科学基金(编号:81271162) 安徽省科技攻关计划项目(编号:1401045013)
【分类号】：R781.4

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