咬合创伤对大鼠颞下颌关节中钙黏蛋白-11表达的影响
发布时间：2018-09-04 08:49
【摘要】：研究背景与目的:临床上常见由牙齿异常接触、咀嚼功能紊乱和医源性因素等导致的咬合干扰产生的咬合创伤。咬合创伤能够对颞下颂关节产生各种生物学影响,近年来研究发现钙黏蛋白-11(cadherin-ll,Cad-ll,CDH-ll)与颞下颌关节发生、发育及颞下颂关节紊乱病等密切相关。本研究通过建立大鼠咬合创伤模型,观察咬合创伤前后颞下颂关节的组织形态学变化及IL-6、TNF-α、Cad-11在颞下颌关节中的分布表达情况等,探讨咬合创伤影响颞下颌关节的机制,以及Cad-11在此过程中的相关作用,为颞下颂关节紊乱病的病因机制、临床诊断与治疗等研究提供新的靶点和思路。实验方法:本实验将45只8周龄雄性Wistar大鼠(体重约180-220g)随机分为实验组一(Experiment group Ⅰ,EG Ⅰ)、实验组二(Experiment groupⅡ,EGⅡ)和对照组(Control group,CG)三大组。EG Ⅰ中,通过在大鼠下颌右侧第一磨牙粘接高出鄈平面1.0mm的3/4钴铬金属冠建立咬合创伤模型,并分为咬合创伤1w、2w及4w组,每组各5只大鼠;EGⅡ为EGⅠ各组基础上分别去除咬合创伤2周,即1w+2w、2w+2w及4w+2w组,每组各5只大鼠;对照组为无咬合创伤的大鼠,每组各3只大鼠。所有大鼠于相应时间点处死并取材,标本经固定、脱钙、包埋等程序后,正中矢状面制备5.0μm厚的连续切片。通过苏木精一伊红染色(hematoxylin-eosin staining,HE染色)进行颞下颂关节的组织形态学观察;通过免疫组织化学染色(immunohistochemical staining,IHC 染色)检测 IL-6、TNF-α、Cad-11的表达情况,并用ImagePro-Plus6.2软件行相应的平均光密度值计算,通过Graphpad Prism6.x.C统计软件进行相应的数据统计分析。实验结果:第一部分大鼠咬合创伤模型的建立本实验及课题组前期实验均已验证,此高出鄈平面1.Omm的3/4钴铬金属冠固位良好、稳定可靠,成功对Wistar大鼠牙周组织造成了明显的病理损伤,成功建立了咬合创伤模型。第二部分咬合创伤大鼠颞下颌关节的组织形态学观察咬合创伤大鼠的颞下颂关节HE染色组织形态学分析显示:EG Ⅰ中1w组大鼠的颞下颌关节出现明显炎症反应,主要表现为吞噬小泡增多、关节软骨细胞肿大;2w及4w组炎症情况减轻,但出现软骨细胞簇状增生、软骨基质粘液样改变等病理变化;EGⅡ中大鼠的颞下颂关节与对照组正常组织无明显差异。第三部分咬合创伤大鼠颞下颌关节中IL-6、TNF-α和Cad-11的表达变化咬合创伤大鼠的颞下颂关节免疫组织化学染色结果显示:CG中IL-6、TNF-α和Cad-11呈弱阳性表达,主要分布于髁突肥大细胞层、肥大细胞层与增殖层交界处;EG Ⅰ中1w组大鼠颞下颂关节的肥大细胞层、肥大细胞层与增殖层交界处可见 IL-6、TNF-α 和 Cad-11 呈强阳性表达(P0.05);2w 组及 4w 组的 IL-6、TNF-α和Cad-11与1w组相比表达强度均减弱(P0.05);EGⅡ中1w+2w组的髁突组织内IL-6、TNF-α和Cad-11的表达量高于各自对照组(P0.05),但2w+2w和4w+2w组内IL-6、TNF-α和Cad-11的表达与各自的对照组无统计学差异(P0.05)。结论:通过粘接3/4钴铬金属冠的方式建立的大鼠咬合创伤模型稳定可靠,适用于咬合创伤相关研究。在咬合创伤早期,大鼠颞下颂关节炎症反应明显,随后炎症反应减弱,造成颞下颂关节病理性损伤;去除咬合创伤后炎症反应减弱,有利于颞下颌关节的组织愈合。Cad-11在炎症反应早期可能协同IL-6、TNF-α等经典促炎症因子共同促进咬合创伤促发的颞下颌关节的炎症反应;另外,Cad-11还可能在咬合创伤所致的颞下颌关节退行性变等过程中发挥重要作用。
[Abstract]:BACKGROUND AND OBJECTIVE: Occlusal trauma caused by occlusal interference caused by abnormal tooth contact, masticatory dysfunction and iatrogenic factors is common in clinic. Occlusal trauma can exert various biological effects on infratemporal song joint. In recent years, studies have found that cadherin-ll (Cad-ll, CDH-ll) and temporomandibular joint occur and develop. In this study, we established a rat model of occlusal trauma, observed the histomorphological changes of the joint before and after occlusal trauma and the distribution and expression of IL-6, TNF-a, Cad-11 in the temporomandibular joint, and explored the mechanism of occlusal trauma affecting the temporomandibular joint and the mechanism of Cad-11 in the process. Methods: Forty-five eight-week-old male Wistar rats (weighing about 180-220 g) were randomly divided into experimental group I (EG I), experimental group II (EG II) and control group (Contr I). The occlusal trauma model was established by attaching 3/4 cobalt-chromium crowns of 1.0 mm above the union plane to the right mandibular first molars of rats, and was divided into 1 w, 2 W and 4 W occlusal trauma groups with 5 rats in each group. All the rats were sacrificed at the corresponding time points, and the specimens were fixed, decalcified and embedded. The median sagittal plane was made into 5.0 micron thick serial sections. The histomorphology of the infratemporal euphoria joint was observed by hematoxylin-eosin staining (HE staining). The expression of IL-6, TNF-a and Cad-11 was detected by immunohistochemical staining (IHC staining). The average optical density was calculated by ImagePro-Plus 6.2 software. The data were analyzed by Graphpad Prism6.x.C statistical software. The model of occlusal injury in Wistar rats was established successfully. Part two: Histological observation of temporomandibular joint in occlusal trauma rats Histomorphological analysis of HE staining of the infratemporal song joint in rats with occlusal trauma showed that the temporomandibular joint of the rats in the 1st week group of EG I had obvious inflammatory reaction, mainly manifested as increased phagocytic vesicles and enlarged articular chondrocytes; the inflammation of the rats in the 2nd and 4th weeks groups was alleviated, but there were some pathological changes, such as chondrocyte cluster proliferation and cartilage matrix mucoid changes In the third part, the expression of IL-6, TNF-a and Cad-11 in TMJ of occlusal trauma rats was changed. The immunohistochemical staining results of TMJ of occlusal trauma rats showed that IL-6, TNF-a and Cad-11 in CG were weakly positive, mainly distributed in condyle. There were strong positive expressions of IL-6, TNF-a and Cad-11 in the mast cell layer and the junction between the mast cell layer and the proliferative layer in the 1st week group of EG I, and the expression of IL-6, TNF-a and Cad-11 in the 1st week group and the 4th week group were all weaker than those in the 1st week group of EG II (P 0.05). The expressions of IL-6, TNF-a and Ca-11 in condylar tissue of rats were higher than those of the control group (P 0.05), but there was no significant difference in the expressions of IL-6, TNF-a and Ca-11 between the two groups (P 0.05). In the early stage of occlusal trauma, the inflammatory reaction of rat's infratemporal song joint was obvious, and then the inflammatory reaction was weakened, which resulted in pathological injury of rat's infratemporal song joint. After removing occlusal trauma, the inflammatory reaction was weakened, which was beneficial to the tissue healing of temporomandibular joint. In addition, Cad-11 may play an important role in the process of temporomandibular joint degeneration caused by occlusal trauma.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R782.6
本文编号：2221597
[Abstract]:BACKGROUND AND OBJECTIVE: Occlusal trauma caused by occlusal interference caused by abnormal tooth contact, masticatory dysfunction and iatrogenic factors is common in clinic. Occlusal trauma can exert various biological effects on infratemporal song joint. In recent years, studies have found that cadherin-ll (Cad-ll, CDH-ll) and temporomandibular joint occur and develop. In this study, we established a rat model of occlusal trauma, observed the histomorphological changes of the joint before and after occlusal trauma and the distribution and expression of IL-6, TNF-a, Cad-11 in the temporomandibular joint, and explored the mechanism of occlusal trauma affecting the temporomandibular joint and the mechanism of Cad-11 in the process. Methods: Forty-five eight-week-old male Wistar rats (weighing about 180-220 g) were randomly divided into experimental group I (EG I), experimental group II (EG II) and control group (Contr I). The occlusal trauma model was established by attaching 3/4 cobalt-chromium crowns of 1.0 mm above the union plane to the right mandibular first molars of rats, and was divided into 1 w, 2 W and 4 W occlusal trauma groups with 5 rats in each group. All the rats were sacrificed at the corresponding time points, and the specimens were fixed, decalcified and embedded. The median sagittal plane was made into 5.0 micron thick serial sections. The histomorphology of the infratemporal euphoria joint was observed by hematoxylin-eosin staining (HE staining). The expression of IL-6, TNF-a and Cad-11 was detected by immunohistochemical staining (IHC staining). The average optical density was calculated by ImagePro-Plus 6.2 software. The data were analyzed by Graphpad Prism6.x.C statistical software. The model of occlusal injury in Wistar rats was established successfully. Part two: Histological observation of temporomandibular joint in occlusal trauma rats Histomorphological analysis of HE staining of the infratemporal song joint in rats with occlusal trauma showed that the temporomandibular joint of the rats in the 1st week group of EG I had obvious inflammatory reaction, mainly manifested as increased phagocytic vesicles and enlarged articular chondrocytes; the inflammation of the rats in the 2nd and 4th weeks groups was alleviated, but there were some pathological changes, such as chondrocyte cluster proliferation and cartilage matrix mucoid changes In the third part, the expression of IL-6, TNF-a and Cad-11 in TMJ of occlusal trauma rats was changed. The immunohistochemical staining results of TMJ of occlusal trauma rats showed that IL-6, TNF-a and Cad-11 in CG were weakly positive, mainly distributed in condyle. There were strong positive expressions of IL-6, TNF-a and Cad-11 in the mast cell layer and the junction between the mast cell layer and the proliferative layer in the 1st week group of EG I, and the expression of IL-6, TNF-a and Cad-11 in the 1st week group and the 4th week group were all weaker than those in the 1st week group of EG II (P 0.05). The expressions of IL-6, TNF-a and Ca-11 in condylar tissue of rats were higher than those of the control group (P 0.05), but there was no significant difference in the expressions of IL-6, TNF-a and Ca-11 between the two groups (P 0.05). In the early stage of occlusal trauma, the inflammatory reaction of rat's infratemporal song joint was obvious, and then the inflammatory reaction was weakened, which resulted in pathological injury of rat's infratemporal song joint. After removing occlusal trauma, the inflammatory reaction was weakened, which was beneficial to the tissue healing of temporomandibular joint. In addition, Cad-11 may play an important role in the process of temporomandibular joint degeneration caused by occlusal trauma.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R782.6
【参考文献】
相关期刊论文 前5条
1 甘春兰;陈婉;蔡捷;赵文青;吴煜;;Cadherin-11与大鼠牙齿发育关系的初步研究[J];广西医科大学学报;2015年01期
2 黑伟红;刘志坚;白丁;郑泽彬;贺红;;大鼠牙移动性根吸收组织内钙黏蛋白-11的表达[J];武汉大学学报(医学版);2012年03期
3 李延武,陈力,马肃;成年鼠髁突增龄性变化的组织学和组织化学研究[J];口腔医学研究;2004年06期
4 马绪臣,张震康;颞下颌关节紊乱综合征的命名和诊断分类[J];中华口腔医学杂志;1998年04期
5 傅开元,马绪臣,张震康,孙开华,王晶,朱晓滨;创伤致家兔颞下颌关节滑膜和软骨超微结构的改变[J];中华口腔医学杂志;1998年01期
相关硕士学位论文 前1条
1 甘春兰;Cadherin-11对大鼠牙髓细胞迁移、黏附和分化的影响[D];广西医科大学;2015年
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