Naa10p在口腔鳞癌患者唾液、血清中的表达及临床意义研究

发布时间：2018-09-07 07:11

【摘要】：目的:检测N端α位乙酰基转移酶10蛋白(N-α-acetyltransferase 10 protein,Naa10p)、癌胚抗原(Carcinoembryonic antigen,CEA)在口腔鳞状细胞癌(Oral Squamous Cell Carcinoma,OSCC)患者、癌前病变患者和正常人唾液、血清中的表达,探讨Naa10p、CEA与OSCC发生发展的关系及联合检测唾液中Naa10p、CEA对OSCC的诊断价值。方法:应用ELISA法检测76例OSCC患者、30例癌前病变患者和40例正常人唾液中Naa10p、CEA,血清中Naa10p的表达情况,并分析OSCC患者唾液中Naa10p、CEA,血清中Naa10p的表达与年龄、性别、族别、分化程度、临床分期、肿瘤转移等临床参数的相关性。以病理学检查结果为金标准绘制ROC曲线,评价各指标单项检测及唾液中Naa10p、CEA联合检测对于诊断OSCC的诊断性能。结果:(1)OSCC组、癌前病变组、正常对照组唾液中Naa10p、CEA的表达两两之间差异均有统计学意义(P0.05)。无论是血清还是唾液,OSCC组中Naa10p表达均显著高于正常对照组。无论是OSCC组还是正常对照组,唾液中Naa10p表达均显著高于血清中表达。(2)OSCC组唾液中Naa10p表达与分化程度相关,血清中Naa10p表达与年龄相关,唾液中CEA表达与临床分期、淋巴结转移相关(P0.05),其余均无统计学意义(P0.05)。(3)绘制ROC曲线,唾液中Naa10p的ROC曲线下面积(0.924)明显大于血清中Naa10p(0.713),差异有统计学意义(P0.05);唾液、血清中Naa10p的灵敏度、特异度、准确度分别为(85.40%、85.00%、85.30%)、(74.20%、65.00%、72.10%)。唾液中Naa10p、CEA单项和联合检测的灵敏度、特异度、准确度、阳性预测值、阴性预测值分别为(81.70%、72.50%、78.00%、81.70%、72.50%)、(88.30%、47.50%、72.00%、71.60%、26.90%)和(91.70%、80.00%、87.00%、87.30%、86.50%);唾液中Naa10p、CEA和联合检测的ROC曲线下面积分别为0.822、0.736、0.911,联合检测的ROC曲线下面积均大于任一单项检测,差异有统计学意义(P0.05)。结论:(1)Naa10p在OSCC患者唾液和血清中均高表达,唾液中Naa10p的表达与肿瘤分化程度相关,提示Naa10p可能与OSCC的发生发展有关,且唾液中Naa10p表达高于血清中,提示检测唾液中Naa10p的表达比检测血清中Naa10p对OSCC的诊断更具有临床意义。(2)CEA在OSCC患者唾液中高表达,表明唾液中CEA亦可协助口腔鳞癌的诊断。(3)唾液中Naa10p、CEA联合检测有助于提高口腔鳞癌的诊断性能,是检查口腔鳞癌较为理想的生物标志物组合。
[Abstract]:Objective: to detect the expression of N-terminal 伪 -acetyltransferase 10 protein,Naa10p (N- 伪 -acetyltransferase 10 protein,Naa10p) and carcinoembryonic antigen (Carcinoembryonic antigen,CEA) in saliva and serum of patients with oral squamous cell carcinoma (Oral Squamous Cell Carcinoma,OSCC), precancerous lesions and normal controls. To investigate the relationship between Naa10p,CEA and OSCC and the diagnostic value of Naa10p,CEA in saliva for OSCC. Methods: the expression of Naa10p in saliva of 76 patients with OSCC and 30 patients with precancerous lesion and 40 normal controls was detected by ELISA method. The expression of Naa10p in saliva of OSCC patients and age, sex, race, differentiation degree were analyzed. The correlation of clinical parameters such as clinical stage and tumor metastasis. The ROC curve was drawn according to the results of pathological examination as gold standard to evaluate the diagnostic performance of single item detection and Naa10p,CEA combined detection in saliva for diagnosis of OSCC. Results: (1) the expression of Naa10p,CEA in saliva of OSCC group, precancerous lesion group and normal control group were significantly different (P0.05). The expression of Naa10p in both serum and saliva OSCC group was significantly higher than that in normal control group. The expression of Naa10p in saliva was significantly higher than that in serum in both OSCC group and normal control group. (2) the expression of Naa10p in saliva of OSCC group was correlated with the degree of differentiation, the expression of Naa10p in serum was correlated with age, and the expression of CEA in saliva was correlated with clinical stage. The area under ROC curve of Naa10p in saliva (0.924) was significantly larger than that in serum (0.713), the difference was statistically significant (P0.05), the sensitivity, specificity and accuracy of Naa10p in saliva and serum were 85.30%, 85.30%, 75.20% and 72.10%, respectively. Sensitivity, specificity, accuracy, positive predictive value of single and combined detection of Naa10p,CEA in saliva, The negative predictive values were 72.50% (72.50%, 78.00%) and 72.50%), (88.30% (47.50%) and 71.60% (26.90%) and (91.7080.0087.30%, 87.3086.50%), respectively, and the area under the ROC curve of Naa10p,CEA in saliva and joint detection was 0.8220.7360.911, the area under the ROC curve of joint detection was larger than that of any single test, the difference was statistically significant (P0.05). Conclusion: (1) Naa10p is highly expressed in saliva and serum of OSCC patients. The expression of Naa10p in saliva is related to the degree of tumor differentiation, suggesting that Naa10p may be related to the occurrence and development of OSCC, and the expression of Naa10p in saliva is higher than that in serum. It is suggested that detecting the expression of Naa10p in saliva is more important than detecting Naa10p in serum for the diagnosis of OSCC. (2) the high expression of CEA in the saliva of OSCC patients. The results suggest that CEA in saliva can also help to diagnose oral squamous cell carcinoma. (3) combined detection of Naa10p,CEA in saliva is helpful to improve the diagnostic performance of oral squamous cell carcinoma and is an ideal biomarker combination for detection of oral squamous cell carcinoma.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.8

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