人牙髓干细胞和根尖乳头干细胞体外分化能力的对比研究
发布时间：2018-09-14 10:50
【摘要】：目的 对比研究人牙髓干细胞(DPSCs)和根尖乳头干细胞(SCAP)的体外生长特性、增殖及矿化能力。方法 采用酶消化法体外培养人DPSCs和SCAP,诱导分化培养基诱导细胞成骨/成牙本质向分化,流式细胞仪检测特异性标记物,茜素红染色检测矿化程度,逆转录PCR(RT-PCR)检测分化标记物表达。结果 人DPSCs和SCAP为成纤维细胞样贴壁生长,SCAP增殖率较高;两种细胞表达特异性间充质干细胞(MSCs)标记物:CD34、CD45(-),CD90、CD105、CD146(+),基质细胞抗原1(STRO-1)、八聚体转录因子4(OCT-4)(+),SCAP特异性标记物CD24(+)。成骨诱导3周可形成明显钙化结构,SCAP钙化能力较强。成骨诱导2周2种细胞均可表达分化标记物:骨涎蛋白、骨钙素、牙本质涎磷蛋白,且随诱导时间表达逐渐增加。结论 人DPSCs和SCAP均具有间充质干细胞典型特征,可分化为成牙本质样细胞,是牙源性组织工程的可靠干细胞来源。
[Abstract]:Objective to compare the growth characteristics, proliferation and mineralization of human dental pulp stem cells (DPSCs) and apical papilla stem cells (SCAP) in vitro. Methods Human DPSCs and SCAP, culture medium were used to induce osteoblast / dentin differentiation, flow cytometry was used to detect specific markers and alizarin red staining was used to detect mineralization. The expression of differentiation markers was detected by reverse transcription PCR (RT-PCR). Results the proliferation rate of human DPSCs and SCAP was higher than that of fibroblast-like adherent cells, and the two kinds of cells expressed specific mesenchymal stem cell (MSCs) markers: CD34-CD45 (-) CD90 CD105- CD146 (), stromal cell antigen 1 (STRO-1), OCT-4) () transcription factor 4 (OCT-4) () specific marker CD24 (). After 3 weeks of osteogenic induction, the calcified structure of SCAP was found to be highly calcified. Two weeks after osteogenic induction, two kinds of cells expressed differentiation markers: bone sialoprotein, osteocalcin, dentin sialophosphorus protein, and the expression increased gradually with the induction time. Conclusion both human DPSCs and SCAP have typical characteristics of mesenchymal stem cells and can differentiate into odontoblast cells, which is a reliable source of stem cells for odontogenic tissue engineering.
【作者单位】： 云南省第一人民医院口腔内科;昆明医科大学第一附属医院心内科;
【分类号】：R781.3
本文编号：2242491
[Abstract]:Objective to compare the growth characteristics, proliferation and mineralization of human dental pulp stem cells (DPSCs) and apical papilla stem cells (SCAP) in vitro. Methods Human DPSCs and SCAP, culture medium were used to induce osteoblast / dentin differentiation, flow cytometry was used to detect specific markers and alizarin red staining was used to detect mineralization. The expression of differentiation markers was detected by reverse transcription PCR (RT-PCR). Results the proliferation rate of human DPSCs and SCAP was higher than that of fibroblast-like adherent cells, and the two kinds of cells expressed specific mesenchymal stem cell (MSCs) markers: CD34-CD45 (-) CD90 CD105- CD146 (), stromal cell antigen 1 (STRO-1), OCT-4) () transcription factor 4 (OCT-4) () specific marker CD24 (). After 3 weeks of osteogenic induction, the calcified structure of SCAP was found to be highly calcified. Two weeks after osteogenic induction, two kinds of cells expressed differentiation markers: bone sialoprotein, osteocalcin, dentin sialophosphorus protein, and the expression increased gradually with the induction time. Conclusion both human DPSCs and SCAP have typical characteristics of mesenchymal stem cells and can differentiate into odontoblast cells, which is a reliable source of stem cells for odontogenic tissue engineering.
【作者单位】： 云南省第一人民医院口腔内科;昆明医科大学第一附属医院心内科;
【分类号】：R781.3
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