关于初期稳定性对即刻种植成功率影响的动物实验研究
发布时间：2018-09-18 14:09
【摘要】：目的：从实验的角度观察初期稳定性对HA涂层、软组织水平、根形植体即刻种植成功率的影响,以期为临床手术中的操作提供理论依据。 方法： 1、建立动物模型： 四只成年杂种狗双侧下颌后牙区四个种植位点随机分为两组：实验组和对照组。实验组：差级备孔后植入BLB植体(北京莱顿生物材料有限公司),控制其ISQ值小于50,植入力矩值小于20N；对照组：精确备孔后植入BLB植体,控制其ISQ值大于65,植入力矩值大于30N。在植入当时及第四周、八周、十二周测量植体ISQ值并记录；同期拍摄狗颅脑CT并观察种植体周围骨密度。 2、术后第十二周处死实验动物 (1)肉眼观察：种植体周围情况 (2)光镜下观察：在种植位点取2.5x1.5xl cm3骨标本(包含种植体)共十六个做硬组织切片后进行甲苯胺蓝染色(或伊红美兰染色)。光学显微镜下观察骨密度并计算种植体—骨结合率(图1)。 3、统计学分析：各组数据行正态性检验,若数据为正态分布,则行方差齐性检验和t检验；若数据不为正态分布,则行wilcoxon秩和检验。 结果： 1、大体标本观察：所有种植体均无松动、脱落现象；种植体周围局部粘膜健康,未发现充血、肿胀等炎性症状。用金属杆轻轻敲打种植体,敲击声音清脆,表示已种植成功。 2、影像学检查：植入当天CT显示示对照组与实验组种植体周围骨密度有显著差异(图2)；植入后第四周CT显示对照组与实验组种植体周围骨密度无显著差异(图3)；植入后第八周CT显示对照组与实验组种植体骨密度无显著差异(图4)。植入后第十二周CT显示对照组与实验组种植体骨密度无显著差异。 3、组织学检查：甲苯胺蓝染色后,见即刻拔牙后遗留空腔大部分已有新生骨长入,可见明显的哈佛氏系统(图5)。新生的编织骨延种植体表面螺纹蔓延沉积(图6),种植体与牙槽骨为骨性结合,可见成骨细胞零星排列,散落分布(图7)。种植体与牙槽骨之间的结合呈间断性,大部分切片显示种植体底部与牙槽骨之间有空腔。在少数种植体螺纹边缘未有骨结合处,伊红美兰染色中发现有未及骨化的纤维组织(图8)。 4、统计学分析：各组数据行正态性检验和方差齐性检验,确定各组数据均为正态分布、方差齐性,行方差分析P0.05,差异无统计学意义,可认为两组间利,植体—骨结合率无区别。 结论：即刻种植BLB植体初期的ISQ值在28到49区间及68至75区间,对应植入力矩值在10-20N以内及30N以上,其成功率无显著差别。
[Abstract]:Objective: to observe the effect of initial stability on HA coating, soft tissue level and immediate implant success rate in order to provide theoretical basis for clinical operation. Methods: 1. Establish animal model: four adult mongrel dogs were randomly divided into two groups: experimental group and control group. Experimental group: BLB implant (Beijing Leiden Biomaterials Co., Ltd.) was implanted after the preparation of the hole, the ISQ value was less than 50, and the implant torque value was less than 20N. in the control group, the BLB implant was implanted after accurate preparation, the ISQ value was more than 65, and the implant torque value was more than 30N. At the time of implantation and four weeks, eight weeks and twelve weeks, the ISQ values of the implant were measured and recorded. At the same time, CT was photographed and bone mineral density around implant was observed. 2. 12 weeks after operation, experimental animals were killed (1) naked eye observation: (2) condition around implant (2) under light microscope: under implantation Sixteen 2.5x1.5xl cm3 bone specimens (including implants) were taken for hard tissue sections and stained with toluidine blue (or erethromethylene blue staining). Bone mineral density (BMD) was observed under optical microscope and implant-bone bonding rate (Fig. 1) was calculated (Fig. 1). Statistical analysis: the data of each group were examined by normality test, and if the data were normal distribution, homogeneity test of variance and t test were performed. If the data is not normal distribution, the wilcoxon rank sum test is performed. Results: 1. Gross observation: no loosening and abscission were observed in all implants and no inflammatory symptoms such as congestion and swelling were found in the local mucosa around the implants. The implants were lightly struck with metal rods, and the sound was clear and crisp, indicating that the implant had been successfully planted. 2. Imaging examination: on the day of implantation, CT showed that there was a significant difference in bone mineral density between the control group and the experimental group (Fig. 2); There was no significant difference in bone mineral density between the control group and the experimental group by CT at the 4th week after implantation (Fig.3), but by CT at the 8th week after implantation, there was no significant difference in BMD between the control group and the experimental group (Fig. 4). CT showed that there was no significant difference in bone density between the control group and the experimental group 12 weeks after implantation. An obvious Harvard system can be seen (Fig. 5). The newly formed braided bone extended the surface of the implant (Fig. 6), the implant and alveolar bone were osseous, osteoblasts were scattered and scattered (Fig. 7). The bonding between implant and alveolar bone was intermittent, and most sections showed that there was a cavity between the implant bottom and alveolar bone. Unossified fibrous tissue was found in a few implants with unossified fibrous tissue at the edge of the implant thread (Fig. 8). 4. Statistical analysis: the data of each group were tested for normality and homogeneity of variance. The data of each group were all normal distribution, homogeneity of variance, analysis of variance (P0.05), the difference was not statistically significant, it can be considered that there is no difference between the two groups, the implant and bone bonding rate. Conclusion: the initial ISQ values of immediate BLB implants are between 28 to 49 and 68 to 75, and the corresponding implant torque values are within 10 ~ 20 N and more than 30 N, and there is no significant difference in the success rate.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R783.6
本文编号：2248156
[Abstract]:Objective: to observe the effect of initial stability on HA coating, soft tissue level and immediate implant success rate in order to provide theoretical basis for clinical operation. Methods: 1. Establish animal model: four adult mongrel dogs were randomly divided into two groups: experimental group and control group. Experimental group: BLB implant (Beijing Leiden Biomaterials Co., Ltd.) was implanted after the preparation of the hole, the ISQ value was less than 50, and the implant torque value was less than 20N. in the control group, the BLB implant was implanted after accurate preparation, the ISQ value was more than 65, and the implant torque value was more than 30N. At the time of implantation and four weeks, eight weeks and twelve weeks, the ISQ values of the implant were measured and recorded. At the same time, CT was photographed and bone mineral density around implant was observed. 2. 12 weeks after operation, experimental animals were killed (1) naked eye observation: (2) condition around implant (2) under light microscope: under implantation Sixteen 2.5x1.5xl cm3 bone specimens (including implants) were taken for hard tissue sections and stained with toluidine blue (or erethromethylene blue staining). Bone mineral density (BMD) was observed under optical microscope and implant-bone bonding rate (Fig. 1) was calculated (Fig. 1). Statistical analysis: the data of each group were examined by normality test, and if the data were normal distribution, homogeneity test of variance and t test were performed. If the data is not normal distribution, the wilcoxon rank sum test is performed. Results: 1. Gross observation: no loosening and abscission were observed in all implants and no inflammatory symptoms such as congestion and swelling were found in the local mucosa around the implants. The implants were lightly struck with metal rods, and the sound was clear and crisp, indicating that the implant had been successfully planted. 2. Imaging examination: on the day of implantation, CT showed that there was a significant difference in bone mineral density between the control group and the experimental group (Fig. 2); There was no significant difference in bone mineral density between the control group and the experimental group by CT at the 4th week after implantation (Fig.3), but by CT at the 8th week after implantation, there was no significant difference in BMD between the control group and the experimental group (Fig. 4). CT showed that there was no significant difference in bone density between the control group and the experimental group 12 weeks after implantation. An obvious Harvard system can be seen (Fig. 5). The newly formed braided bone extended the surface of the implant (Fig. 6), the implant and alveolar bone were osseous, osteoblasts were scattered and scattered (Fig. 7). The bonding between implant and alveolar bone was intermittent, and most sections showed that there was a cavity between the implant bottom and alveolar bone. Unossified fibrous tissue was found in a few implants with unossified fibrous tissue at the edge of the implant thread (Fig. 8). 4. Statistical analysis: the data of each group were tested for normality and homogeneity of variance. The data of each group were all normal distribution, homogeneity of variance, analysis of variance (P0.05), the difference was not statistically significant, it can be considered that there is no difference between the two groups, the implant and bone bonding rate. Conclusion: the initial ISQ values of immediate BLB implants are between 28 to 49 and 68 to 75, and the corresponding implant torque values are within 10 ~ 20 N and more than 30 N, and there is no significant difference in the success rate.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R783.6
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