釉质脱矿后再矿化的体外研究

发布时间：2018-09-18 16:31

【摘要】：目的：通过模拟固定矫治后的牙釉质表面脱矿,使用显微硬度仪进行定量测量,并用扫描电镜观察釉质表面形态结构,比较几种再矿化产品对脱矿釉质的再矿化效果,为其应用提供实验依据。方法：选取因正畸治疗而拔除的健康前磨牙90颗,随机分为6组,每组15个样本,分别进行不同的处理：A组：不作任何处理；B组：脱矿处理；C组：脱矿后浸泡在人工唾液中；D组：脱矿后浸泡在人工唾液中,牙齿颊面涂布氟保护漆；E组：脱矿后浸泡在人工唾液中,牙齿颊面涂布多乐氟；F：脱矿后浸泡在人工唾液中,牙齿颊面涂布GC护牙素。应用显微硬度仪测量所有样本釉质表面的显微硬度值,应用统计学软件SPSS17.0对所测数据进行单因素方差分析,组间的两两比较用LSD-t检验,P0.05认为有统计学意义。另外从每组样本中随机挑选样本在扫描电镜下观察釉质表面形态结构。结果：①显微硬度值结果比较A组的显微硬度值最高,B组的显微硬度值最低,D组、E组和F组的显微硬度值均高于C组,F组的显微硬度值高于D组和E组,以上差异均具有统计学意义(P=0.000,P0.05),D组和E组的显微硬度值差异无统计学意义(P=0.355,P0.05)。②扫描电镜观察结果A组表现为正常釉质表面形态,B组呈为明显的脱矿状态,釉柱溶解破坏呈蜂窝状,C组、D组、E组和F组在釉质表面均可见矿物质沉积,其中C组矿物质沉积较少,F组矿物质沉积最明显,D组和E组矿物质沉积水平相当。结论：①GC护牙素、多乐氟、0.1%氟保护漆和人工唾液均能使脱矿釉质再矿化,使釉质表面显微硬度值增高,并能改善釉质表面微观形态。②GC护牙素、多乐氟、0.1%氟保护漆均能增强脱矿釉质的再矿化效果,较单纯使用人工唾液时的再矿化效果具有明显优势,其中GC护牙素对显微硬度的增高效果最为突出,其次为多乐氟和0.1%氟保护漆,多乐氟和0.1%氟保护漆对脱矿釉质的再矿化效果尚未发现明显差异。③正常牙釉质的硬度最高,脱矿釉质短时间内经再矿化处理也难以恢复至脱矿前釉质硬度。
[Abstract]:Objective: to compare the demineralization effect of several remineralization products on enamel surface by imitating the demineralization of enamel surface after fixation, measuring quantitatively by microhardness instrument, observing the morphology of enamel surface by scanning electron microscope (SEM), and comparing the effect of several remineralization products on demineralization of enamel. To provide experimental basis for its application. Methods: 90 healthy premolars extracted by orthodontic treatment were randomly divided into 6 groups, 15 samples in each group were treated with different treatments: group B: demineralization; Group C: immerse in artificial saliva after demineralization, group D: after demineralization, immerse in artificial saliva, coated with fluorine protective paint E group: after demineralization, immerse in artificial saliva, and coat tooth buccal surface with Dorafur; F: soaked in artificial saliva after demineralization, and coated with GC dentin on the buccal surface of teeth. The microhardness of all samples was measured by microhardness instrument, and the data were analyzed by single factor ANOVA using statistical software SPSS17.0. The comparison between groups was considered statistically significant by LSD-t test (P0.05). In addition, samples were randomly selected from each group to observe the surface morphology of enamel under scanning electron microscope (SEM). Results the microhardness values of group A and group B were higher than those of group C and group F, respectively, and the values of group E and group F were higher than those of group C and group E. the microhardness values of group A were higher than those of group D and group E, and the values of group F were higher than those of group C and group E. There was no significant difference in microhardness between group D and group E (P < 0. 355P 0.05). 2 SEM observation showed that group A showed normal enamel surface morphology and group B showed obvious demineralization state, and there was no significant difference in microhardness between group D and group E (P < 0. 355P 0.05). The dissolution and destruction of enamel post were honeycombed in group C, group D, group E and group F, and mineral deposits were found on the surface of enamel. The mineral deposits in group C were less than those in group F, and the levels of mineral deposition in group D and group E were the same. Conclusion 1% GC dentin, 0.1% fluoride-protective paint and artificial saliva can make demineralized enamel mineralization, increase the microhardness of enamel surface, and improve the morphology of enamel surface .2GC. The effect of demineralization of demineralized enamel was enhanced by 0.1% fluoride-protective paint, which was superior to that of artificial saliva alone, and the effect of GC dentin on the increase of microhardness was the most obvious. The remineralization effect of Dorafur and 0.1% fluorine protective lacquer on demineralized enamel was not found to be significant difference. 3. The hardness of normal enamel was the highest. The enamel hardness of demineralized enamel before demineralization is difficult to recover after remineralization in a short time.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.5

【参考文献】