来曲唑对涎腺腺样囊性癌细胞ACC-LM移植瘤影响的研究

发布时间：2018-10-07 18:20

【摘要】：[目的]观察芳香化酶抑制剂来曲唑对裸鼠腺样囊性癌ACC-LM移植瘤抗肿瘤作用,探讨芳香化酶抑制剂抑瘤作用机制,为临床治疗涎腺腺样囊性癌提供理论依据。[方法]构建裸鼠腺样囊性癌移植瘤模型。通过右腋皮下注射1×106个腺样囊性癌细胞,观察成瘤情况。种瘤两周后随机分为三组,每组8只,分别为对照组(生理盐水,0.2ml/只/2d)、二甲基亚砜(DMSO)组(DMSO,0.01ml/kg/2d)、来曲唑组(来曲唑,5mg/kg/2d+DMSO,0.01ml/kg/2d)。记录裸鼠重量变化及瘤体体积,3天/次。种瘤两周后开始药物干预,四周时处死,剥离瘤体,称重计算抑瘤率,绘制肿瘤生长曲线。取部分新鲜瘤体组织进行流式细胞术检测,比较各组凋亡率差异。部分瘤体组织进行伊红苏木素染色(HE染色)和芳香化酶p450免疫组织化学染色,比较各组肿瘤生长状况及芳香化酶P450在肿瘤细胞中的表达。采用SPSS13.0统计软件进行统计分析。[结果]1.瘤体重量及抑瘤率结果:对照组、DMSO组、来曲唑组瘤体重量分别为1.50±0.39g、1.52±0.26g、0.65±0.18g。对照组及DMSO组与来曲唑组之间有差别(P0.05),对照组与DMSO组之间无差别(P0.05),来曲唑抑瘤率为56.67%。2.各组肿瘤体积变化:对照组与DMSO组瘤体变化大,而来曲唑组变化较小。对照组及DMSO组与来曲唑组之间有显著差异(P0.01),差别有统计学意义(P0.05);对照组与DMSO组之间差别无统计学意义(P0.05)。3.凋亡率结果:对照组、DMSO组、来曲唑组凋亡率分别为4.6±1.8%、5.7±1.5%、13.6±4.2%,对照组与DMSO组之间差别无统计学意义(P0.05),来曲唑与对照组、DMSO组均有显著性差异(P0.01)。4.灰度值结果:对照组、DMSO组、来曲唑组的平均灰度值分别为87.75±3.73、88.37±3.85、95.88±4.24。对照组与DMSO组之间差别无统计学意义(P0.05),来曲唑与对照组及DMSO组均有显著性差异(P0.01)。[结论]1.芳香化酶抑制剂来曲唑能够抑制裸鼠腺样囊性癌移植瘤的生长。2.来曲唑能降低裸鼠腺样囊性癌移植瘤内P450的表达。
[Abstract]:[objective] to observe the antitumor effect of aromatase inhibitor letrozole on ACC-LM xenografts of adenoid cystic carcinoma in nude mice, and to explore the mechanism of aromatase inhibitor inhibiting tumor, and to provide theoretical basis for clinical treatment of salivary adenoid cystic carcinoma. Methods the transplanted tumor model of adenoid cystic carcinoma in nude mice was established. The tumorigenesis was observed by subcutaneous injection of 1 脳 106 adenoid cystic carcinoma cells in the right axilla. After two weeks, they were randomly divided into three groups: control group (normal saline 0.2 ml / rat / 2 d), dimethyl sulfoxide (DMSO) group (DMSO,0.01ml/kg/2d) and letrozole group (letrozole 5 mg / kg / 2 d DMSO,0.01ml/kg/2d). The weight change and tumor volume of nude mice were recorded for 3 days. After two weeks of drug intervention, the tumor was killed, the tumor was stripped, the tumor inhibition rate was calculated, and the tumor growth curve was drawn. Some fresh tumor tissues were detected by flow cytometry to compare the apoptotic rate of each group. Some tumor tissues were stained with eosin (HE) and aromatase p450 immunohistochemical staining to compare the tumor growth status and the expression of aromatase P450 in tumor cells. SPSS13.0 statistical software was used for statistical analysis. [result] 1. Results: the tumor weight and tumor inhibition rate in the control group and letrozole group were 1.50 卤0.39g / L and 1.52 卤0.26g/ 0.65 卤0.18grespectively. There was significant difference between control group and DMSO group and letrozole group (P0.05), but there was no difference between control group and DMSO group (P0.05). The inhibitory rate of letrozole was 56.67.2. The change of tumor volume in control group and DMSO group was larger than that in letrozole group. There was significant difference between control group and DMSO group and letrozole group (P0.01), the difference was statistically significant (P0.05), but there was no significant difference between control group and DMSO group (P0.05). 3. Apoptosis rate: the apoptotic rate of control group and letrozole group were 4.6 卤1.8 and 5.7 卤1.5 respectively. There was no significant difference between control group and DMSO group (P0.05). There was significant difference between letrozole group and control group (P0.01). Results: the average gray values of the control group and letrozole group were 87.75 卤3.73 卤88.37 卤3.85 卤95.88 卤4.24, respectively. There was no significant difference between control group and DMSO group (P0.05), letrozole was significantly different from control group and DMSO group (P0.01). [conclusion] 1. Letrozole, an aromatase inhibitor, inhibited the growth of xenografts of adenoid cystic carcinoma in nude mice. Letrozole can reduce the expression of P 450 in xenografts of adenoid cystic carcinoma in nude mice.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.8

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