肥胖复合牙周炎大鼠血清及牙龈中TNF-α、IL-1β水平与胰岛素抵抗的关系

发布时间：2018-10-11 17:39

【摘要】：目的:观察牙周炎复合肥胖大鼠血清及牙龈组织中炎症因子的水平与胰岛素抵抗的关系。方法:取SPF级新生SD大鼠32只,随机分为正常对照组(C)、肥胖组(OB)、牙周炎组(CP)、牙周炎复合肥胖组(OB+CP)(n=8),通过皮下注射谷氨酸钠建立肥胖模型;丝线结扎联合局部接种牙周致病菌(Pg、Ha、Pi、Fn)建立牙周炎模型。采用ELISA法和免疫组化染色法分别检测大鼠血清及牙龈组织中的TNF-α、IL-1β含量,并与胰岛素抵抗指数(HOMA-IR)行相关性分析。结果:与正常对照组相比,各组血清中的TNF-α和IL-1β水平均明显升高(P0.05),其中以OB+CP组升高幅度最大(P0.05);HOMA-IR评分也以OB+CP组最高,肥胖大鼠血清中TNF-α及IL-1β水平与HOMA-IR均呈正相关(rTNF-α=0.854,rIL-1β=0.730)。各组牙龈组织中的TNF-α及IL-1β水平以CP组最高(P0.05),其次为OB+CP组,其TNF-α及IL-1β水平低于CP组(P0.05),但均高于C组和OB组(P0.05);OB组牙龈组织中的TNF-α、IL-1β水平与正常对照组差异均无统计学意义(P0.05)。结论:牙周炎可能通过促进血清中炎症因子的分泌而加剧肥胖机体的胰岛素抵抗。
[Abstract]:Aim: to investigate the relationship between serum and gingival inflammatory factors and insulin resistance in rats with periodontitis and obesity. Methods: 32 new SD rats of SPF grade were randomly divided into (OB), periodontitis group, (CP), periodontitis group and obese (OB CP) (nun8 group. The obese model was established by subcutaneous injection of sodium glutamate. The model of periodontitis was established by ligation of filaments and local inoculation of periodontal pathogenic bacteria (Pg,Ha,Pi,Fn). The contents of TNF- 伪 and IL-1 尾 in serum and gingival tissue of rats were detected by ELISA and immunohistochemical staining, and the correlation between TNF- 伪, IL-1 尾 and insulin resistance index (HOMA-IR) was analyzed. Results: compared with the normal control group, the levels of TNF- 伪 and IL-1 尾 in the serum of each group were significantly increased (P0.05), especially in the OB CP group (P0.05), the HOMA-IR score was the highest in the OB CP group, and the levels of TNF- 伪 and IL-1 尾 in the serum of obese rats were positively correlated with HOMA-IR (rTNF- 伪 = 0.854rIL-1 尾 = 0.730). The levels of TNF- 伪 and IL-1 尾 in gingival tissue of each group were the highest in CP group (P0.05), followed by OB CP group. The levels of TNF- 伪 and IL-1 尾 in gingival tissue were lower than those in CP group (P0.05), but were higher than those in C group and OB group (P0.05). There was no significant difference in TNF- 伪 and IL-1 尾 levels between); OB group and normal control group (P0.05). Conclusion: periodontitis may increase insulin resistance by promoting the secretion of inflammatory factors in serum.
【作者单位】：南方医科大学附属口腔医院·广东省口腔医院;
【基金】：广东省自然科学基金(S2012010010393) 国家自然科学基金(81371151,81271160)
【分类号】：R589.2;R781.42

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