滇重楼总皂苷对涎腺腺样囊性癌ACC-83细胞增殖抑制及其机制的研究

发布时间：2018-10-12 06:38

【摘要】：目的探讨滇重楼总皂苷对涎腺腺样囊性癌ACC-83细胞的增殖抑制作用及其相关机制,为滇重楼治疗涎腺腺样囊性癌提供理论依据。方法通过体外细胞培养,采用CCK-8法检测不同药物质量浓度(5、10、20、40、60、80、100μg·m L-1)的滇重楼总皂苷对ACC-83细胞的增殖抑制效应,采用流式细胞仪检测不同药物质量浓度(25、50、100μg·m L-1)的滇重楼总皂苷对ACC-83细胞的凋亡率,Western blot及逆转录聚合酶链反应法检测巨噬细胞游走抑制因子(MIF)和CD74的表达。结果滇重楼总皂苷可促进ACC-83细胞凋亡,并呈剂量-效应关系。ACC-83细胞中存在MIF和CD74的表达,滇重楼总皂苷可抑制MIF和CD74的表达。结论滇重楼总皂苷对ACC-83细胞的生长有明显抑制作用,可以抑制MIF及CD74的表达,诱导细胞凋亡,为其临床应用提供了依据。
[Abstract]:Objective to investigate the inhibitory effect of total saponins of Dianghui on the proliferation of salivary adenoid cystic carcinoma (ACC-83) cells and its related mechanism, and to provide a theoretical basis for the treatment of salivary adenoid cystic carcinoma. Methods in vitro cell culture, CCK-8 assay was used to detect the inhibitory effect of the total saponins of Rhizoma Diannoides on the proliferation of ACC-83 cells. The apoptosis rate of ACC-83 cells was detected by flow cytometry and the expression of (MIF) and CD74 in macrophages was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results the total saponins could promote the apoptosis of ACC-83 cells in a dose-dependent manner. The expression of MIF and CD74 was found in ACC-83 cells, and the expression of MIF and CD74 was inhibited by the total saponins of Rhizoma Diannoides. Conclusion the total saponins of Rhizoma Diannoides can inhibit the growth of ACC-83 cells, inhibit the expression of MIF and CD74, induce apoptosis, and provide evidence for its clinical application.
【作者单位】：昆明医科大学附属口腔医院口腔颌面外科;口腔疾病研究国家重点实验室国家口腔疾病临床研究中心四川大学华西口腔医院;
【基金】：口腔疾病研究国家重点实验室开放课题(SKLOD2015OF10) 云南省医疗卫生单位内设研究机构科研项目(2016NS115)~~
【分类号】：R285

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