HIF-1α基因介导的牙髓干细胞在体外的成血管作用
发布时间：2018-10-12 13:39
【摘要】：目的探索低氧诱导因子-1α(HIF-1α)基因诱导牙髓干细胞(DPSCs)在体外的成血管作用。方法对HIF-1α进行基因突变,构建突变型、野生型以及对照组的慢病毒载体;体外培养DPSCs;分别用3种慢病毒转染DPSCs后,检测细胞转染效率、目的基因HIF-1α在mRNA及蛋白水平的表达,MTT法检测慢病毒载体对细胞增殖的影响;目的基因转染成功后,q PCR、Western blot法检测HIF-1α调控DPSCs成血管因子的表达。结果 MTT结果表明慢病毒载体对DPSCs的增殖几乎无影响。q PCR和Western blot法检测目的基因HIF-1α成功表达,HIF-1α能够显著上调DPSCs的成血管因子的表达(P0.05)。突变组和野生组的成血管作用明显强于对照组(P0.05),而突变组又优于野生组(P0.05)。结论 HIF-1α基因可以促进DPSCs血管向分化作用。
[Abstract]:Objective to investigate the vascularization of dental pulp stem cell (DPSCs) induced by hypoxia inducible factor-1 伪 (HIF-1 伪) gene in vitro. Methods Lentivirus vectors of mutant type, wild type and control group were constructed by gene mutation of HIF-1 伪, DPSCs; was transfected into DPSCs by three kinds of lentiviruses in vitro, and the transfection efficiency was measured. Objective to detect the expression of HIF-1 伪 gene at the level of mRNA and protein, to detect the effect of lentivirus vector on cell proliferation by MTT method, and to detect the expression of DPSCs angiogenic factor regulated by HIF-1 伪 after gene transfection. Results the results of MTT showed that lentivirus vector had little effect on the proliferation of DPSCs and the expression of target gene HIF-1 伪 was detected successfully by. Q PCR and Western blot. HIF-1 伪 could significantly up-regulate the expression of angiogenic factors of DPSCs (P0.05). The vascularization effect of mutant group and wild group was significantly stronger than that of control group (P0.05), but the mutation group was superior to wild group (P0.05). Conclusion HIF-1 伪 gene can promote the differentiation of DPSCs vessels.
【作者单位】： 安徽医科大学口腔医学院安徽医科大学附属口腔医院安徽省口腔疾病研究中心实验室;
【基金】：国家自然科学基金(编号:31501103)
【分类号】：R781
，
本文编号：2266324
[Abstract]:Objective to investigate the vascularization of dental pulp stem cell (DPSCs) induced by hypoxia inducible factor-1 伪 (HIF-1 伪) gene in vitro. Methods Lentivirus vectors of mutant type, wild type and control group were constructed by gene mutation of HIF-1 伪, DPSCs; was transfected into DPSCs by three kinds of lentiviruses in vitro, and the transfection efficiency was measured. Objective to detect the expression of HIF-1 伪 gene at the level of mRNA and protein, to detect the effect of lentivirus vector on cell proliferation by MTT method, and to detect the expression of DPSCs angiogenic factor regulated by HIF-1 伪 after gene transfection. Results the results of MTT showed that lentivirus vector had little effect on the proliferation of DPSCs and the expression of target gene HIF-1 伪 was detected successfully by. Q PCR and Western blot. HIF-1 伪 could significantly up-regulate the expression of angiogenic factors of DPSCs (P0.05). The vascularization effect of mutant group and wild group was significantly stronger than that of control group (P0.05), but the mutation group was superior to wild group (P0.05). Conclusion HIF-1 伪 gene can promote the differentiation of DPSCs vessels.
【作者单位】： 安徽医科大学口腔医学院安徽医科大学附属口腔医院安徽省口腔疾病研究中心实验室;
【基金】：国家自然科学基金(编号:31501103)
【分类号】：R781
，
本文编号：2266324
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