口腔干预对慢性牙周炎大鼠颈动脉MMP-2，9和血清IL-6影响的研究
发布时间：2018-10-15 06:50
【摘要】：目的： 本实验通过建立SD大鼠的慢性牙周炎(chronic periodontitis,CP)和动脉粥样硬化(atherosclerosis, As)模型,模拟慢性牙周炎患者临床治疗过程中可能采取的干预措施,研究不同口腔干预对CP大鼠动脉粥样硬化发生、发展的影响。 方法： 将50只雄性SD大鼠编号后根据数字随机表随机分成3大组：A组(正常对照组)7只、B组(As组)8只、C组(CP组)35只,牙周炎建模成功后,再将C组按照不同口腔干预措施分为C1组(自然进程)、C2组(单纯机械治疗)、C3组(药物治疗)、C4-1组(拔牙)和C4-2组(拔牙+抗生素),每组均为7只。按上述措施进行口腔干预,酶联免疫吸附法(ELISA)检测5个不同时间点(建模成功后1、3、5、7和9周)血清IL-6含量；建模成功后9周末处死动物,免疫组化法检测颈动脉血管组织中MMP-2,9的含量。 结果： 1、血清学显示：随着时间推移,B和C1组血清IL-6含量一直呈升高趋势,且显著高于A组(P0.01)；各干预组(C2、C3、C4-1、C4-2组)IL-6含量逐渐升高,并在建模成功后5周达到峰值(C2:62.3±14.3、C3:58.2±8.7、C4-1:127.0±29.9、C4-2：120.6±23.1),且高于B组(43.4±7.5)(P0.01),其中C4-1和C4-2组升高最明显,均接近B组的3倍,C2和C3组接近其1.5倍,之后逐渐下降。最终,C2组(28.6±8.1)、C3组(40.8±15.1)和C4-2组(32.7±11.1)低于B组(66.3±7.8)(P0.05),且C2组最低；C4-1组(72.8±16.4)虽下降明显,但仍为最高。 2、免疫组化法检测显示：B、C1和C4-1组MMP-2,9大量阳性表达,且阳性染色深度均明显高于A组(MMP-2,9灰度值分别为：183.0±2.0、181.3±2.0),灰度值差异有统计学意义(P0.01),其中C4-1组阳性染色深度最强,B和C1组依次降低(C4-1：123.1±2.9、121.0±3.2；B：126.3±2.0、124.8±2.8；C1：139.8±2.2、139.7±3.2)(P0.01)；C2、C3和C4-2组阳性染色深度均明显低于C1组(C2：169.3±3.4、169.7±2.3；C3：148.9±1.7、145.1±2.5；C4-2：157.7±1.2、155.8±2.7)(P0.01),C2组阳性染色最弱。 结论： 1、成功建立了SD大鼠的慢性牙周炎和动脉粥样硬化模型。 2、单纯慢性牙周炎SD大鼠任其牙周炎症自然发展,发生As的风险可能会逐渐增高。 3、单纯慢性牙周炎SD大鼠,牙周机械治疗在短时间内可能会增加As的发病风险,但是长时间观察,其风险逐渐降低,对血管的改善程度效果最明显。 4、单纯慢性牙周炎SD大鼠,直接拔牙风险较高,而拔牙后给予全身应用抗炎药可以明显降低As发生、发展风险。
[Abstract]:Objective: to establish the (atherosclerosis, As) model of chronic periodontitis (chronic periodontitis,CP) and atherosclerosis in SD rats, and to simulate the possible intervention measures in the course of clinical treatment of chronic periodontitis. To study the effect of different oral intervention on the occurrence and development of atherosclerosis in CP rats. Methods: fifty male SD rats were randomly divided into three groups: group A (normal control group), group B (As group, 8 rats) and group C (CP group, 35 rats). Group C was divided into C1 group (natural process), C2 group (simple mechanical treatment), C3 group (drug therapy), C4-1 group (extraction of teeth) and C4-2 group (extraction antibiotics) according to different oral intervention measures. Each group had 7 rats. According to the above measures, the serum IL-6 levels were measured by Elisa (ELISA) at 5 different time points (1 ~ 3 ~ 5 ~ 5 weeks after modeling), and the animals were killed at the end of 9 weeks after modeling. The content of MMP-2,9 in carotid artery was detected by immunohistochemical method. Results: 1. Serological results showed that the serum IL-6 levels in groups B and C1 increased with time and were significantly higher than those in group A (P0.01), and the content of IL-6 in each intervention group (C2C3C3-C4-1C4-2) gradually increased. The peak value of C2: 62.3 卤14.3C3: 58.2 卤8.7 C4-1: 127.0 卤29.9C4-2: 12.6 卤23.1 was higher than that of group B (43.4 卤7.5) (P0.01). The increase of C4-1 and C4-2 was the most obvious, nearly 3 times of that of group B, 1.5 times of group C2 and C3, and then decreased gradually. Finally, C _ 2 group (28.6 卤8.1), C _ 3 group (40.8 卤15.1) and C _ 4-2 group (32.7 卤11.1) were lower than B group (66.3 卤7.8) (P0.05), and C _ 2 group (72.8 卤16.4) was the lowest. The depth of positive staining was significantly higher in group A than in group A (MMP-2,9 gray value: 183.0 卤2.0181.3 卤2.0), and the difference was statistically significant (P0.01). The depth of positive staining was the strongest in group C4-1, and decreased in group B and C1 (C4-1: 123.1 卤2.9121.0 卤3.2Bw 126.3 卤2.0124.8 卤2.8124.8 卤2.8C1w 139.8 卤2.2139.7 卤3.2) (P0.01). The depth of positive staining in C _ 2 C _ 3 and C _ 4-2 groups was significantly lower than that in C _ 1 group (C _ 2: 169.3 卤3.4169.7 卤2.3 C _ 3: 148.9 卤1.7145.1 卤2.5C4-2: 157.7 卤1.2155.8 卤2.7) (P0.01). Conclusion: 1. The models of chronic periodontitis and atherosclerosis in SD rats were successfully established. 2. Chronic periodontitis SD rats were allowed to develop their periodontitis naturally. The risk of As may increase gradually. 3. In SD rats with chronic periodontitis, periodontal mechanical therapy may increase the risk of As in a short period of time, but the risk decreases gradually after a long period of observation. (4) SD rats with chronic periodontitis had a higher risk of direct extraction of teeth, and the risk of development of As could be significantly reduced by systemic application of anti-inflammatory drugs after extraction of teeth.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.42
[Abstract]:Objective: to establish the (atherosclerosis, As) model of chronic periodontitis (chronic periodontitis,CP) and atherosclerosis in SD rats, and to simulate the possible intervention measures in the course of clinical treatment of chronic periodontitis. To study the effect of different oral intervention on the occurrence and development of atherosclerosis in CP rats. Methods: fifty male SD rats were randomly divided into three groups: group A (normal control group), group B (As group, 8 rats) and group C (CP group, 35 rats). Group C was divided into C1 group (natural process), C2 group (simple mechanical treatment), C3 group (drug therapy), C4-1 group (extraction of teeth) and C4-2 group (extraction antibiotics) according to different oral intervention measures. Each group had 7 rats. According to the above measures, the serum IL-6 levels were measured by Elisa (ELISA) at 5 different time points (1 ~ 3 ~ 5 ~ 5 weeks after modeling), and the animals were killed at the end of 9 weeks after modeling. The content of MMP-2,9 in carotid artery was detected by immunohistochemical method. Results: 1. Serological results showed that the serum IL-6 levels in groups B and C1 increased with time and were significantly higher than those in group A (P0.01), and the content of IL-6 in each intervention group (C2C3C3-C4-1C4-2) gradually increased. The peak value of C2: 62.3 卤14.3C3: 58.2 卤8.7 C4-1: 127.0 卤29.9C4-2: 12.6 卤23.1 was higher than that of group B (43.4 卤7.5) (P0.01). The increase of C4-1 and C4-2 was the most obvious, nearly 3 times of that of group B, 1.5 times of group C2 and C3, and then decreased gradually. Finally, C _ 2 group (28.6 卤8.1), C _ 3 group (40.8 卤15.1) and C _ 4-2 group (32.7 卤11.1) were lower than B group (66.3 卤7.8) (P0.05), and C _ 2 group (72.8 卤16.4) was the lowest. The depth of positive staining was significantly higher in group A than in group A (MMP-2,9 gray value: 183.0 卤2.0181.3 卤2.0), and the difference was statistically significant (P0.01). The depth of positive staining was the strongest in group C4-1, and decreased in group B and C1 (C4-1: 123.1 卤2.9121.0 卤3.2Bw 126.3 卤2.0124.8 卤2.8124.8 卤2.8C1w 139.8 卤2.2139.7 卤3.2) (P0.01). The depth of positive staining in C _ 2 C _ 3 and C _ 4-2 groups was significantly lower than that in C _ 1 group (C _ 2: 169.3 卤3.4169.7 卤2.3 C _ 3: 148.9 卤1.7145.1 卤2.5C4-2: 157.7 卤1.2155.8 卤2.7) (P0.01). Conclusion: 1. The models of chronic periodontitis and atherosclerosis in SD rats were successfully established. 2. Chronic periodontitis SD rats were allowed to develop their periodontitis naturally. The risk of As may increase gradually. 3. In SD rats with chronic periodontitis, periodontal mechanical therapy may increase the risk of As in a short period of time, but the risk decreases gradually after a long period of observation. (4) SD rats with chronic periodontitis had a higher risk of direct extraction of teeth, and the risk of development of As could be significantly reduced by systemic application of anti-inflammatory drugs after extraction of teeth.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.42
【参考文献】
相关期刊论文 前8条
1 周卉;欧阳翔英;段晋瑜;段向青;;牙周炎症对冠心病患者CRP水平影响的初探[J];北京口腔医学;2009年04期
2 任秀云;石学雪;孙丽莉;岳姿洁;林牧;常乐;;口腔干预对牙周炎大鼠动脉粥样硬化影响的研究[J];北京口腔医学;2012年05期
3 赵筱芩;孟姝;吴亚菲;陈宇;葛颂;;基质金属蛋白酶-2和基质金属蛋白酶-3在大鼠牙周炎模型中的表达[J];华西口腔医学杂志;2006年03期
4 吴亚菲,赵筱芩,陈宇,范新平,肖晓蓉,朱xC;不同方法建立大鼠实验性牙周炎模型的比较研究[J];四川大学学报(医学版);2003年04期
5 张明珠;梁景平;李超伦;姜云涛;束蓉;;牙龈卟啉单胞菌感染兔动脉粥样硬化模型的建立[J];上海交通大学学报(医学版);2007年06期
6 丁鳌;王晓燕;唐昊U，
本文编号：2271713
本文链接：https://www.wllwen.com/yixuelunwen/kouq/2271713.html
最近更新
教材专著
