促肝细胞再生磷酸酶1基因对舌癌细胞侵袭的影响

发布时间：2018-10-15 08:35

【摘要】：目的:探讨促肝细胞再生磷酸酶1(phosphatase of regenerating liver cell-1,PRL-1)对舌癌细胞侵袭能力的影响及其可能的机制。方法:应用si RNA-PRL-1慢病毒载体转染处理舌癌TCA8113细胞株后,分别采用荧光定量PCR和蛋白质印迹检测PRL-1基因和基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)m RNA和蛋白水平;采用Transwell小室模型实验检测癌细胞的侵袭能力。结果:si RNA-PRL-1慢病毒转染组癌细胞PRL-1在RNA(0.425 0±0.010 0)和蛋白(2.720 0±0.110 0)水平明显下调(P=0.000,P=0.000),同时MMP-2及MMP-9在RNA(0.562 2±0.180 0,0.617 1±0.100 0)及蛋白(0.592 4±0.010 0,0.476 2±0.020 0)表达水平降低(P=0.024,P=0.010,P=0.000,P=0.000);Transwell实验si RNA-PRL-1慢病毒转染组细胞通过数(64.33±4.04)明显减少(P=0.000)。结论:si RNA-PRL-1转染可抑制舌癌细胞侵袭,其机制可能与PRL-1基因下调MMP-2及MMP-9的表达有关。
[Abstract]:Aim: to investigate the effect of promoting hepatocyte regeneration phosphatase 1 (phosphatase of regenerating liver cell-1,PRL-1) on the invasion of tongue cancer cells and its possible mechanism. Methods: after transfection with si RNA-PRL-1 lentivirus vector, the levels of PRL-1 gene, matrix metalloproteinase-2 (matrix metalloproteinase-2,MMP-2), matrix metalloproteinase-9 (matrix metalloproteinase-9,MMP-9) m RNA) and protein were detected by fluorescence quantitative PCR and Western blot, respectively. The invasive ability of cancer cells was detected by Transwell chamber model experiment. Results: the levels of PRL-1 and protein in RNA (0.425 卤0.010) and protein (2.720 0 卤0.110 0) in si RNA-PRL-1 lentivirus transfected group were significantly down-regulated (P0. 000), while the expression levels of MMP-2 and MMP-9 in RNA (0.562 2 卤0.180 0 + 0. 617 1 卤0.100 0) and protein (0.592 4 卤0.010 00. 476 2 卤0.020 0) were significantly decreased (P0. 024 P0. 010 + P0. 000); Transwell transfection group). The number of excess (64.33 卤4.04) was significantly decreased (P0. 000). Conclusion: si RNA-PRL-1 transfection can inhibit the invasion of tongue cancer cells, and the mechanism may be related to the down-regulation of MMP-2 and MMP-9 expression by PRL-1 gene.
【作者单位】：重庆医科大学附属口腔医院口腔颌面外科口腔疾病与生物医学重庆市重点实验室;
【基金】：重庆市重点课题资助项目(编号:2009-1-33) 重庆市卫生局重点资助项目(编号:2013-1-030)
【分类号】：R739.8

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