高龋学龄前儿童变形链球菌的水平传播方式

发布时间：2018-10-15 13:43

【摘要】：目的研究高龋学龄前儿童变形链球菌的水平传播方式,为儿童龋病早期预防提供依据。方法收集20名4~5岁高龋学龄前儿童口腔中牙菌斑标本进行厌氧培养,通过细菌形态学观察、生物化学鉴定、聚合酶链反应(PCR)扩增变形链球菌基因的特异性片段葡糖基转移酶B(gtfB)以及随机引物聚合酶链反应(AP-PCR)等方法对获得的变形链球菌临床分离株进行鉴定。结果从20例高龋学龄前儿童口腔中分离获得200株变形链球菌临床分离株,经生物化学鉴定排除其中19株,获得181株变形链球菌临床分离株,变形链球菌的特异性基因gtfB的PCR扩增181株变形链球菌临床分离株均鉴定为变形链球菌,AP-PCR发现37种不同基因型,其中6名儿童携带1种基因型,11名儿童携带2种基因型,2名儿童携带3种基因型,1名儿童携带4种基因型,有2名不同班级儿童携带一种相同基因型。结论获得37株不同基因型变形链球菌临床分离株,高龋学龄前儿童间未发现变形链球菌水平传播。
[Abstract]:Objective to study the horizontal transmission of Streptococcus mutans in preschool children and to provide evidence for early prevention of caries in children. Methods 20 dental plaque specimens from the oral cavity of 20 children aged 4 to 5 years old with high caries were collected for anaerobic culture. The bacterial morphology was observed and identified by biochemistry. Polymerase chain reaction (PCR) was used to amplify the specific fragment of Streptococcus mutans gene glucosyltransferase (B (gtfB) and random primer polymerase chain reaction (AP-PCR) to identify the clinical isolates of Streptococcus mutans. Results 200 strains of Streptococcus mutans were isolated from the oral cavity of 20 children with high caries. Among them, 19 strains were excluded by biochemistry, and 181 strains of Streptococcus mutans were obtained. The clinical isolates of Streptococcus mutans were identified by PCR amplification of the specific gene gtfB of Streptococcus mutans and 37 different genotypes were identified by AP-PCR. Among them, 6 children carry one genotype, 11 children carry 2 genotypes, 2 children carry 3 genotypes, 1 child carries 4 genotypes, and 2 children in different classes carry one of the same genotypes. Conclusion among 37 clinical isolates of Streptococcus mutans with different genotypes, no horizontal transmission of Streptococcus mutans was found among preschool children with high caries.
【作者单位】：解放军总医院口腔科;解放军总医院微生物科;
【基金】：国家自然科学基金(81041028,81271191)~~
【分类号】：R788.1

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