生物活性玻璃对L929细胞增殖及骨向分化的影响

发布时间：2018-10-15 13:06

【摘要】：目的通过检测不同浓度的生物活性玻璃浸提液对小鼠L929细胞的增殖活性、细胞周期和骨向分化的作用,为生物活性玻璃在牙周组织再生领域的应用提供基础实验依据。方法本实验研究分为两部分:1生物活性玻璃对L929细胞增殖的影响。制备生物活性玻璃浸提液,以10%小牛血清的RPMI1640完全培养液稀成不同浓度的生物活性玻璃(bioactive glass,BG)浸提液培养L929细胞24h、48h和72h后,采用四唑盐(MTT)法和流式细胞术(FCM)法检测不同浓度的生物活性玻璃浸提液对L929细胞增殖活性和细胞周期的影响。2生物活性玻璃对L929细胞骨向分化的影响。采用碱性磷酸酶(ALP)活性测定的方法,分别在24h、48h和72h检测不同浓度的生物活性玻璃浸提液对体外培养L929细胞骨向分化的影响。结果MTT和ALP法测定结果均显示培养L929细胞24h、48h和72h后,随时间延长,0.0625g/L~1.25g/L组的OD值均高于对照组,差异有统计学意义(P0.05),以0.0625g/L浓度组的OD值最高(P0.05);2.5g/L组的OD值较对照组稍低,差异无统计学意义(P0.05);5g/L组与对照组相比OD值显著降低,差异有统计学意义(P0.05)。FCM实验结果与上述实验结果相符,BG浸提液浓度为0.0625g/L时细胞增殖指数(PI)最高,浓度为5g/L时PI值最低(P0.05)。结论1生物活性玻璃在一定浓度范围内可促进L929成纤维细胞的增殖及骨向化。2生物活性玻璃在低浓度时促进L929细胞增殖和骨向分化,高浓度时抑制L929细胞的增殖和骨向分化。
[Abstract]:Objective to investigate the effects of different concentrations of bioactive glass extract on the proliferation, cell cycle and bone differentiation of mouse L929 cells, and to provide a basic experimental basis for the application of bioactive glass in the field of periodontal tissue regeneration. Methods the experiment was divided into two parts: 1 the effect of bioactive glass on the proliferation of L 929 cells. The bioactive glass extract was prepared and the L929 cells were incubated with 10% bovine serum RPMI1640 complete culture medium with different concentrations of bioactive glass (bioactive glass,BG) for 48 h and 72 h. The effects of different concentrations of bioactive glass extract on the proliferation activity and cell cycle of L929 cells were detected by (MTT) and flow cytometry. 2 the effects of bioactive glass on bone differentiation of L929 cells were studied. The effects of different concentrations of bioactive glass extract on bone differentiation of L929 cells in vitro were determined by alkaline phosphatase (ALP) assay at 24 h and 72 h, respectively. Results the results of MTT and ALP assay showed that the OD value of 0.0625g/L~1.25g/L group was higher than that of control group (P0.05), the OD value of 0.0625g/L group was the highest (P0.05), the OD value of 2.5g/L group was slightly lower than that of control group. There was no significant difference (P0.05); compared with the control group, the OD value of 5g/L group was significantly lower than that of the control group (P0.05). FCM experimental results were consistent with the above experimental results, the highest (PI) cell proliferation index (PI) when the concentration of BG extract solution was 0.0625g/L, PI value was the lowest when 5g/L concentration was P05). Conclusion (1) Bioactive glass can promote the proliferation and osteotropism of L929 fibroblasts in a certain concentration range. (2) Bioactive glass can promote the proliferation and bone differentiation of L929 cells at low concentration, and inhibit the proliferation and bone differentiation of L929 cells at high concentration.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781.42

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