Th17及其相关因子在不同牙周炎症状态龈沟液中的检测

发布时间：2018-10-17 19:17

【摘要】：目的:探讨Th17及其相关因子在慢性牙周炎发病中的作用。方法:选择46例牙周科患者分为4组:慢性牙周炎活动型位点组、慢性牙周炎静止型位点组、牙龈炎组和健康对照组,应用酶联免疫法检测各组龈沟液中IL-17、RORC2、IFN-γ和IL-4的含量并进行分析。结果:IL-17和RORC2的表达趋势相似,活动型位点组明显高于其他3组,静止型位点组明显高于健康对照组;健康对照组的IFN-γ明显低于其他3组;活动型位点组IL-4明显低于其他3组;IL-17和RORC2呈正相关,IL-17和IL-4呈负相关;IL-17、RORC2、IFN-γ与临床指标BI、PD和AL呈正相关,IL-4与BI、PD和AL呈负相关。结论:Th17在慢性牙周炎活动型中占优势,随着附着丧失增加,龈沟液中含量升高;IFN-γ可能参与了牙龈炎症和非炎症的状态转化。
[Abstract]:Objective: to investigate the role of Th17 and its related factors in the pathogenesis of chronic periodontitis. Methods: 46 patients with periodontitis were divided into 4 groups: chronic periodontitis active locus group, chronic periodontitis static locus group, gingivitis group and healthy control group. The contents of IL-17,RORC2,IFN- 纬 and IL-4 in gingival crevicular fluid were detected by enzyme linked immunosorbent assay (Elisa). Results: the expression trend of IL-17 and RORC2 was similar, the expression of IFN- 纬 in the active locus group was significantly higher than that in the other three groups, and the IFN- 纬 level in the stationary locus group was significantly higher than that in the healthy control group. IL-4 in active locus group was significantly lower than that in other three groups, IL-17 and RORC2 were positively correlated, IL-17 and IL-4 were negatively correlated, IL-17,RORC2,IFN- 纬 was positively correlated with BI,PD and AL, IL-4 was negatively correlated with BI,PD and AL. Conclusion: Th17 is dominant in active type of chronic periodontitis, and the content of IFN- 纬 in gingival crevicular fluid increases with the increase of attachment, and IFN- 纬 may be involved in the transformation of gingival inflammation and non-inflammatory state.
【作者单位】：武汉大学口腔医学院牙周科;口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室;
【基金】：湖北省卫生计生科研基金(编号:WJ2015MB040)
【分类号】：R781.42

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