降钙素对大鼠上颌扩弓初期影响的研究
发布时间：2018-11-04 13:35
【摘要】：[目的]正畸治疗中,上颌扩弓可以有效的纠正患者牙弓水平方向发育不足引起的错(?)畸形,然而多方面因素的影响使得上颌扩弓的效果不稳定,存在一定的复发率。降钙素是甲状腺滤泡旁细胞分泌产生的一种多肽类激素,可以调节全身钙离子流,促进钙盐沉积以及骨质改建。然而降钙素对上颌扩弓的影响尚无相关研究。本实验拟通过建立大鼠上颌扩弓模型,探讨降钙素对大鼠上颌扩弓的影响,以便为将来能否应用于临床提供参考。[方法]第一部分:上颌扩弓动物模型的建立。实验选用24只4周龄SD大鼠,完全随机分为实验组与对照组。0.016英寸澳丝弯置成二眼圈簧扩弓装置,末端插入实验组大鼠第一、二磨牙牙间隙内,光固化复合树脂将扩弓装置固定于大鼠磨牙舌侧,所有大鼠腭中缝两侧植入种植钉,以便观察腭中缝扩展量,通过双侧第一磨牙颊尖以及两颗种植体之间的距离变化观察扩弓效果。第二部分:降钙素对大鼠上颌扩弓早期效果影响的研究,建模方法与第一部分相同。24只4周龄SD大鼠,随机分为实验组与对照组。实验组大鼠每天15IU/kg浓度降钙素腹腔注射,对照组大鼠等量生理盐水每天腹腔注射。通过双侧第一磨牙颊尖以及两侧微种植钉间距离的变化,观察降钙素对扩弓效果的影响。第三部分:降钙素对大鼠上颌扩展初期腭中缝改建影响的研究,对第二部分实验扩弓结束的24只大鼠,两侧第一磨牙连线处制作组织脱矿切片,进行相关染色,观察腭中缝骨质改建情况。[结果]1.两眼圈簧矫治器以及种植钉植入会对大鼠健康产生一定影响(P0.05);实验组大鼠双侧第一磨牙颊尖最外侧距离以及双侧种植钉距离较对照组明显增宽(P0.05)。2.降钙素的作用下实验组大鼠双侧第一磨牙颊尖最外侧距离以及双侧种植钉距离较对照组明显增宽(P0.05)。3.组织形态学观察到实验组大鼠(注射降钙素)腭中缝区域成骨细胞较对照组多(P0.05),腭中缝软骨宽度亦明显增加,骨质改建更加明显。[结论]1.两眼圈簧扩弓矫治器成功建立了大鼠上颌扩弓模型。2.腭中缝两侧植入微种植钉是一种简便、可靠、准确观测腭中缝实际扩展量的方法。3.降钙素能够促进上颌扩弓的效果。4.降钙素在腭中缝扩展初期能促进骨质的改建。
[Abstract]:[objective] in orthodontic treatment, maxillary arch dilatation can effectively correct the malfunction caused by dental arch underdevelopment. Deformity, however, the effect of maxillary arch expansion is unstable due to various factors, and there is a certain recurrence rate. Calcitonin is a polypeptide hormone secreted by paraflosa cells of thyroid gland, which can regulate calcium ion flow, promote calcium salt deposition and bone remodeling. However, the effect of calcitonin on maxillary arch dilatation has not been studied. The purpose of this study was to investigate the effect of calcitonin on maxillary arch dilatation in rats by establishing a rat model of maxillary arch dilatation in order to provide a reference for clinical application in the future. Methods: the first part: the establishment of maxillary arcuate animal model. Twenty-four 4-week-old SD rats were randomly divided into two groups: the experimental group and the control group. The 0.016 inch Australian wire was bent into a two-ring Reed reaming device, and the end was inserted into the first and second molar space of the experimental group. Light cured composite resin fixed the arch expansion device on the lingual side of the rat molars, and implant pins were implanted on both sides of the palatal suture in all rats to observe the amount of expansion of the palatal suture. The effect of arch expansion was observed by the distance between the buccal tip of bilateral first molar and the two implants. The second part: the effect of calcitonin on the early stage of maxillary arch dilatation in rats. The modeling method was the same as that in the first part. 24 4-week-old SD rats were randomly divided into experimental group and control group. Calcitonin was injected intraperitoneally at 15IU/kg concentration in the experimental group and intraperitoneally injected with the same amount of saline in the control group. The effect of calcitonin on arch expansion was observed by the change of distance between buccal tip and bilateral microimplant nail of bilateral first molar. The third part: the effect of calcitonin on palatal suture remodeling in rats at the beginning of maxillary expansion. Tissue demineralization sections were made at the line of the first molar on both sides of 24 rats at the end of the second part of the experimental expansion. To observe the remodeling of palatine suture bone. [result] 1. The distance of bilateral first molar buccal tip and bilateral implant nail in the experimental group was significantly wider than that in the control group (P0.05). The distance between the buccal tip of bilateral first molar and the distance of bilateral implant nail in the experimental group was significantly wider than that in the control group (P0.05) under the action of calcitonin (P0.05). Histomorphologic observation showed that there were more osteoblasts in the palatine suture region in the experimental group than in the control group (P0.05). The width of palatine raphe cartilage and bone remodeling were also significantly increased in the experimental group (P0.05). [conclusion] 1. The rat maxillary arch expansion model was successfully established with two eye ring Reed reaming appliance. 2. It is a simple, reliable and accurate method to observe the actual expansion of palatine suture by implanting microimplant nail on both sides of palate suture. Calcitonin can promote the effect of maxillary arch expansion. 4. Calcitonin promotes bone remodeling at the beginning of palatine suture expansion.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.5
[Abstract]:[objective] in orthodontic treatment, maxillary arch dilatation can effectively correct the malfunction caused by dental arch underdevelopment. Deformity, however, the effect of maxillary arch expansion is unstable due to various factors, and there is a certain recurrence rate. Calcitonin is a polypeptide hormone secreted by paraflosa cells of thyroid gland, which can regulate calcium ion flow, promote calcium salt deposition and bone remodeling. However, the effect of calcitonin on maxillary arch dilatation has not been studied. The purpose of this study was to investigate the effect of calcitonin on maxillary arch dilatation in rats by establishing a rat model of maxillary arch dilatation in order to provide a reference for clinical application in the future. Methods: the first part: the establishment of maxillary arcuate animal model. Twenty-four 4-week-old SD rats were randomly divided into two groups: the experimental group and the control group. The 0.016 inch Australian wire was bent into a two-ring Reed reaming device, and the end was inserted into the first and second molar space of the experimental group. Light cured composite resin fixed the arch expansion device on the lingual side of the rat molars, and implant pins were implanted on both sides of the palatal suture in all rats to observe the amount of expansion of the palatal suture. The effect of arch expansion was observed by the distance between the buccal tip of bilateral first molar and the two implants. The second part: the effect of calcitonin on the early stage of maxillary arch dilatation in rats. The modeling method was the same as that in the first part. 24 4-week-old SD rats were randomly divided into experimental group and control group. Calcitonin was injected intraperitoneally at 15IU/kg concentration in the experimental group and intraperitoneally injected with the same amount of saline in the control group. The effect of calcitonin on arch expansion was observed by the change of distance between buccal tip and bilateral microimplant nail of bilateral first molar. The third part: the effect of calcitonin on palatal suture remodeling in rats at the beginning of maxillary expansion. Tissue demineralization sections were made at the line of the first molar on both sides of 24 rats at the end of the second part of the experimental expansion. To observe the remodeling of palatine suture bone. [result] 1. The distance of bilateral first molar buccal tip and bilateral implant nail in the experimental group was significantly wider than that in the control group (P0.05). The distance between the buccal tip of bilateral first molar and the distance of bilateral implant nail in the experimental group was significantly wider than that in the control group (P0.05) under the action of calcitonin (P0.05). Histomorphologic observation showed that there were more osteoblasts in the palatine suture region in the experimental group than in the control group (P0.05). The width of palatine raphe cartilage and bone remodeling were also significantly increased in the experimental group (P0.05). [conclusion] 1. The rat maxillary arch expansion model was successfully established with two eye ring Reed reaming appliance. 2. It is a simple, reliable and accurate method to observe the actual expansion of palatine suture by implanting microimplant nail on both sides of palate suture. Calcitonin can promote the effect of maxillary arch expansion. 4. Calcitonin promotes bone remodeling at the beginning of palatine suture expansion.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.5
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