纯钛表面氨基等离子体改性对MG-63细胞成骨特性的影响

发布时间：2018-11-04 08:27

【摘要】：目的：研究不同氨基等离子体聚合改性方法处理的纯钛钛片对MG-63细胞的成骨特性表达的影响。方法：以体外培养的MG-63细胞为研究对象，使用cw，p(10%)，p（30%），p（60%），cw+p（30%）五种不同氨基等离子聚合的方法对纯钛钛片表面进行改性，，与MG-63细胞共培养，于1d，4d，7d检测ALP的活性，在矿化诱导培养基的诱导下14d对矿化结节进行染色，7d检测骨钙素的浓度及RUNX-2的表达。结果：1.氨基等离子聚合改性对纯钛钛片表面共培养的MG-63细胞在贴壁早期的ALP活性无明显促进作用，而在细胞进入增殖期后，显著提高了ALP的生成；2.氨基等离子聚合改性在矿化诱导培养基的作用下对纯钛钛片表面共培养的MG-63细胞增加了细胞外基质的矿化，形成较多的钙盐沉积，进一步促进了成骨的过程；3.氨基等离子聚合改性促进了纯钛钛片表面共培养的MG-63细胞骨钙素的形成；4.氨基等离子聚合改性明显增加了纯钛钛片表面共培养的MG-63细胞中成骨相关基因RUNX-2的表达，激活成骨方向的细胞通路，及上下游作用靶点，从多个方面促进成骨。结论：1.氨基等离子体聚合改性能增加纯钛钛片表面MG-63细胞成骨特性的表达；2.以p(60%)方法进行氨基等离子聚合改性的纯钛钛片最为突出，表现出最显著的优越性。
[Abstract]:Aim: to study the effect of pure titanium tablets treated with different amino plasma polymerization methods on the expression of osteogenic characteristics of MG-63 cells. Methods: the surface of pure titanium sheet was modified by cw,p (10%), p (30%), p (60%), cw p (30%) with five different amino plasma polymerization methods. After co-culture with MG-63 cells, the activity of ALP was detected at 1 d, 4 d and 7 d, respectively. The mineralized nodules were stained on the induction medium of mineralization for 14 days, and the concentration of osteocalcin and the expression of RUNX-2 were detected at 7 d. Results: 1. The modification of amino plasma polymerization did not promote the ALP activity of MG-63 cells in the early stage of adhesion, but increased the formation of ALP significantly when the cells entered the proliferative phase. 2. The modification of amino plasma polymerization on MG-63 cells co-cultured on the surface of pure titanium increased the mineralization of extracellular matrix, formed more calcium salt deposition, and further promoted the process of osteogenesis under the action of mineralized induction medium. 3. The modification of amino plasma polymerization promoted the formation of osteocalcin in MG-63 cells cocultured on the surface of pure titanium. 4. The modification of amino plasma polymerization significantly increased the expression of osteoblast-associated gene (RUNX-2) in MG-63 cells co-cultured on the surface of pure titanium, activated the osteoblast pathway in the direction of osteogenesis, and promoted osteogenesis from many aspects. Conclusion 1. The modification of amino plasma polymerization can increase the expression of osteogenic characteristics of MG-63 cells on the surface of pure titanium. 2. The pure titanium sheet modified by p (60%) method of amino plasma polymerization is the most outstanding and has the most remarkable superiority.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.6

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