粗糙纯钛表面掺锶二氧化钛层促进骨整合的动物实验研究
发布时间：2018-11-14 14:07
【摘要】：种植体与骨组织之间的骨结合和良好的初期稳定性是种植手术成功的重要基础。随着口腔种植技术的发展,种植修复的成功率有了极大的提高,然而临床工作中患者的骨缺损情况各异,对于吸烟、牙周病、糖尿病、严重骨缺损的患者,单纯的 SLA(Sandblasted with large grit and acid etched,大颗粒喷砂酸蚀)种植体可能无法满足所有患者的需求。种植体的表面特征是影响骨整合速度和程度的一个重要因素。关于种植体表面的改性有很多,包括物理、化学、生物等方面的改性。近年来,由于抗骨质疏松药物雷尼酸锶的使用,锶表面改性成为研究热点。本研究利用水热处理法对SLA种植体进行掺锶表面改性,进行表征实验和动物实验,来探究掺锶种植体的生物学效应,及其与表征之间的联系,为今后种植体表面改性研究提供实验依据和理论基础。首先将纯钛表面用不同粗细的砂纸打磨抛光,喷砂,双重酸蚀,形成微米多孔状表面(SLA表面)。随后,采用课题组前期研究确立的Sr(OH)□·8H□O水热反应溶液的适宜浓度、温度和时间,制备具有微纳米结构且在一定时间内可持续释放锶的掺锶钛表面(Sr-SLA 表面)(Strontium-Sandblasted with large grit and acidetchedsurface,大颗粒喷砂酸蚀掺锶表面)。场发射扫描电镜结果显示,在微米和亚微米级别上可见Sr-SLA表面和SLA表面都表现出由喷砂和双重酸蚀形成的典型的相似不规则凹痕。在纳米级别上,Sr-SLA表面出现出更多的颗粒样聚集物和凸起,直径约为50nm的致密纳米点,SLA表面无上述纳米点。原子力显微镜结果也显示Sr-SLA表面存在更多的凸起和隆起。表明水热处理在一定程度上保留了钛片经喷砂酸蚀后形成的微米级结构,并新形成了一定的纳米结构。X射线光电子能谱和X射线衍射结果显示,Sr-SLA表面含有钛酸锶,SLA表面不含钛酸锶。电感耦合等离子体质谱结果显示,Sr-SLA表面锶释放量随着时间延长逐渐降低。硬度结果显示Sr-SLA表面和SLA表面没有明显差异。随后,将具有粗糙多孔纯钛掺锶种植体(Sr-SLA种植体)和对照组种植体(SLA种植体)共计64枚种植体同期植入到16只新西兰兔的股骨和胫骨干骺端,每只新西兰兔植入4枚种植体,分别在植入后3周和6周处死实验动物,进行扭力测试和硬组织切片分析。动物实验结果表明,相比于SLA种植体,粗糙多孔纯钛掺锶种植体(Sr-SLA种植体)可促进动物体内早期骨形成。本研究探索水热处理法制备的多孔纯钛掺锶微纳米表面的表征和体内生物学效应,发现掺锶种植体能够提高种植体骨结合,为今后种植体的表面改性提供了实验依据和理论基础。
[Abstract]:The bone bonding between implant and bone tissue and good initial stability are the important basis for the success of implant operation. With the development of dental implant technology, the success rate of implant repair has been greatly improved. However, the clinical work of patients with bone defects are different, for smoking, periodontal disease, diabetes, serious bone defect patients, SLA (Sandblasted with large grit and acid etched, large-particle acid blast implants may not be sufficient for all patients. The surface features of implants are an important factor affecting the speed and degree of bone integration. There are many modifications about implant surface, including physical, chemical, biological and so on. In recent years, strontium surface modification has become a hot topic due to the use of anti-osteoporosis drug strontium ranionate. In this study, the surface modification of strontium doped SLA implants was carried out by hydrothermal treatment to investigate the biological effects of strontium doped implants and their relationship with characterization. To provide experimental and theoretical basis for the study of implant surface modification in the future. Firstly, the pure titanium surface was polished with different thickness sandpaper, sandblasting and double acid etching to form a micron porous surface (SLA surface). Then, the suitable concentration, temperature and time of Sr (OH)-8H-O hydrothermal reaction solution were determined by our previous study. Strontium doped titanium (Sr-SLA surface) with microstructures and sustained release of strontium (Sr-SLA surface) (Sr-doped surface) with large Strontium-Sandblasted with large grit and acidetchedsurface, particles was prepared. The results of field emission scanning electron microscopy (SEM) show that both the Sr-SLA surface and the SLA surface exhibit typical similar irregular indentation formed by sand blasting and double acid etching at the micron and submicron levels. At the nanometer level, there are more granular aggregates and bulges on the surface of Sr-SLA, the diameter of which is about the dense nanowires of 50nm, but there are no such nanoparticles on the surface of SLA. Atomic force microscopy (AFM) results also show that there are more bulges and bulges on the Sr-SLA surface. The results show that the micron structure of titanium wafer formed by sandblasting acid etching is preserved in hydrothermal treatment, and a certain nanometer structure is formed. The results of X-ray photoelectron spectroscopy and X-ray diffraction show that the surface of Sr-SLA contains strontium titanate. The surface of SLA does not contain strontium titanate. The results of inductively coupled plasma mass spectrometry (ICP-MS) show that strontium emission from Sr-SLA surface decreases with time. The hardness results show that there is no obvious difference between Sr-SLA surface and SLA surface. Subsequently, 64 implants with coarse porous pure titanium strontium doped implants (Sr-SLA implants) and control group implants (SLA implants) were implanted into the femur and tibial metaphysis of 16 New Zealand rabbits simultaneously. Four implants were implanted into each New Zealand rabbit. The experimental animals were killed 3 and 6 weeks after implantation respectively. The torsion test and hard tissue analysis were performed. Compared with SLA implants, coarse porous pure titanium implants (Sr-SLA implants) can promote early bone formation. The aim of this study was to investigate the characterization and biological effects of porous pure titanium doped strontium nanocrystalline surfaces prepared by hydrothermal treatment. It was found that strontium doped implants could improve the osseous bonding of implants. It provides experimental and theoretical basis for the surface modification of implants in the future.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.6
本文编号：2331375
[Abstract]:The bone bonding between implant and bone tissue and good initial stability are the important basis for the success of implant operation. With the development of dental implant technology, the success rate of implant repair has been greatly improved. However, the clinical work of patients with bone defects are different, for smoking, periodontal disease, diabetes, serious bone defect patients, SLA (Sandblasted with large grit and acid etched, large-particle acid blast implants may not be sufficient for all patients. The surface features of implants are an important factor affecting the speed and degree of bone integration. There are many modifications about implant surface, including physical, chemical, biological and so on. In recent years, strontium surface modification has become a hot topic due to the use of anti-osteoporosis drug strontium ranionate. In this study, the surface modification of strontium doped SLA implants was carried out by hydrothermal treatment to investigate the biological effects of strontium doped implants and their relationship with characterization. To provide experimental and theoretical basis for the study of implant surface modification in the future. Firstly, the pure titanium surface was polished with different thickness sandpaper, sandblasting and double acid etching to form a micron porous surface (SLA surface). Then, the suitable concentration, temperature and time of Sr (OH)-8H-O hydrothermal reaction solution were determined by our previous study. Strontium doped titanium (Sr-SLA surface) with microstructures and sustained release of strontium (Sr-SLA surface) (Sr-doped surface) with large Strontium-Sandblasted with large grit and acidetchedsurface, particles was prepared. The results of field emission scanning electron microscopy (SEM) show that both the Sr-SLA surface and the SLA surface exhibit typical similar irregular indentation formed by sand blasting and double acid etching at the micron and submicron levels. At the nanometer level, there are more granular aggregates and bulges on the surface of Sr-SLA, the diameter of which is about the dense nanowires of 50nm, but there are no such nanoparticles on the surface of SLA. Atomic force microscopy (AFM) results also show that there are more bulges and bulges on the Sr-SLA surface. The results show that the micron structure of titanium wafer formed by sandblasting acid etching is preserved in hydrothermal treatment, and a certain nanometer structure is formed. The results of X-ray photoelectron spectroscopy and X-ray diffraction show that the surface of Sr-SLA contains strontium titanate. The surface of SLA does not contain strontium titanate. The results of inductively coupled plasma mass spectrometry (ICP-MS) show that strontium emission from Sr-SLA surface decreases with time. The hardness results show that there is no obvious difference between Sr-SLA surface and SLA surface. Subsequently, 64 implants with coarse porous pure titanium strontium doped implants (Sr-SLA implants) and control group implants (SLA implants) were implanted into the femur and tibial metaphysis of 16 New Zealand rabbits simultaneously. Four implants were implanted into each New Zealand rabbit. The experimental animals were killed 3 and 6 weeks after implantation respectively. The torsion test and hard tissue analysis were performed. Compared with SLA implants, coarse porous pure titanium implants (Sr-SLA implants) can promote early bone formation. The aim of this study was to investigate the characterization and biological effects of porous pure titanium doped strontium nanocrystalline surfaces prepared by hydrothermal treatment. It was found that strontium doped implants could improve the osseous bonding of implants. It provides experimental and theoretical basis for the surface modification of implants in the future.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.6
【参考文献】
相关期刊论文 前1条
1 ;Fluorescence microscopic analysis of bone osseointegration of strontium-substituted hydroxyapatite implants[J];Journal of Zhejiang University-Science B(Biomedicine & Biotechnology);2012年05期
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