顺铂调控舌鳞癌细胞增殖和迁移能力的研究

发布时间：2018-11-27 09:31

【摘要】：目的:探讨舌鳞癌细胞(human tongue squamous cell carcinoma cells,SCC)经顺铂化疗后的增殖和迁移能力的改变,及其对细胞增殖相关Nanog基因表达的调控作用。方法:本文应用5、4、3.5μg/m L顺铂分别作用于舌鳞癌细胞系SCC-15,处理时间分别为24、48、72 h(实验组),未经顺铂处理的细胞为对照。流式细胞仪检测细胞凋亡率,q RT-PCR及Western blot检测2组细胞中Nanog的表达情况,划痕实验检测2组细胞迁移能力的变化。结果:经顺铂处理后细胞凋亡率增加,48 h后凋亡率显著增加(P=0.000)。q RT-PCR结果显示24、48、72 h组的Nanog基因相对表达量在顺铂处理的细胞中逐渐增加,48 h Nanog基因表达量与对照组相比,差异具有统计学意义(P=0.000)。Western blot实验结果进一步验证了顺铂处理的舌鳞癌细胞中Nanog蛋白相对表达量较对照组高(P≤0.001)。划痕实验显示经顺铂作用时间越长的实验组,细胞迁移速率越高。结论:顺铂化疗对舌鳞癌细胞增殖能力和迁移能力有调控作用。
[Abstract]:Aim: to investigate the effects of cisplatin chemotherapy on proliferation and migration of tongue squamous cell carcinoma (human tongue squamous cell carcinoma cells,SCC) cells and its regulatory effect on the expression of Nanog gene. Methods: the treatment time of Cisplatin on tongue squamous cell carcinoma cell line SCC-15, was 24 渭 g / mL (experimental group) and without cisplatin treatment for 72 h (control group). The apoptosis rate was detected by flow cytometry, the expression of Nanog was detected by Q RT-PCR and Western blot, and the migration ability was detected by scratch test. Results: the apoptosis rate increased after cisplatin treatment and increased significantly after 48 h treatment. The results showed that the relative expression of Nanog gene increased gradually in the cells treated with cisplatin for 72 h. Compared with the control group, the expression of Nanog gene at 48 h was significantly higher than that in the control group (P < 0.001). The results of P0. 000). Western blot assay further confirmed that the relative expression of Nanog protein in Cisplatin treated tongue squamous cell carcinoma cells was higher than that in the control group (P 鈮，

本文编号：2360229