三七总皂苷壳聚糖温控凝胶对大鼠拔牙创愈合以及BMP-2表达的影响

发布时间：2018-12-17 22:16

【摘要】：目的:由于医学的有限性,目前临床工作中有不少因各种局部或全身因素而不得不拔除患牙的情况,而在牙齿拔除后,拔牙创的愈合进程直接影响患者能否尽早地佩戴义齿,并且拔牙窝改建后的局部牙槽嵴的形态和功能,对义齿的固位和稳定、邻牙的正畸移动以及人工种植牙的骨量条件等均产生影响,因此这一过程是口腔颌面外科、修复、正畸和种植医生共同关注的重要课题。本研究通过动物实验旨在探讨载中药三七总皂苷壳聚糖温控凝胶对大鼠拔牙创愈合的影响,能否有效控制剩余牙槽嵴的吸收,以及检测其对BMP-2表达的影响。方法:3月龄SD雄性大鼠(体重200~250g)48只,分别在大鼠拔牙前9天、6天、3天使用便携式牙钻机将其左下颌中切牙齐龈缘磨除牙冠,使其左下中切牙拔牙前不能与对颌牙建立咬合接触关系,在模型建立阶段死了三只。在第十天将剩余45只大鼠分为五组(高浓度组、中浓度组、低浓度组、空白凝胶组、完全空白组),将左下颌中切牙完整拔除后,分别向前四组大鼠拔牙创内置入提前配制的三七总皂苷壳聚糖凝胶高浓度(2.8mg/ml)、中浓度(1.4mg/ml)、低浓度(0.7mg/ml)、空白凝胶各20ul,空白对照组不放置任何东西。各组大鼠分别于术后7、14、28天后随机选择三只进行心脏灌注固定方法处死。剥离大鼠左右下颌骨,拍左右下颌骨数字牙片,测量各组剩余牙槽嵴的相对长度;制作病理切片,通过HE染色观察拔牙创愈合情况以及免疫组织化学染色检测各组不同时期拔牙创BMP-2的表达,统计分析。结果:1.各组剩余牙槽嵴相对长度的统计结果:7天时,五组数值组间相比,均无统计学差异。14天和28天时,3个载药组与空白凝胶组、完全空白组比,3个载药组数值大于空白凝胶组、完全空白组,均有统计学差异;其中,中浓度组数值大于高浓度组和低浓度组,有统计学意义;而高浓度组与低浓度组比,空白凝胶组与完全空白组相比,数值均无统计学差异。同组在不同时间点,五组剩余牙槽嵴相对长度,第7d与第14d相比,无统计学差异,但第28天数值均小于第7天和第14天,有统计学差异。2.各组BMP-2阳性细胞计数统计结果:在这3个时间点3个载药组与空白凝胶组、完全空白组比,3个载药组数目多于空白凝胶组、完全空白组,均有统计学差异。在这3个时间点3个载药组相比,中浓度组数目多于高组和低组,有统计学意义。3个时间点高浓度组与低浓度组比,空白凝胶组与完全空白组相比,数目均无统计学差异。同组在不同时间点,五组阳性细胞计数峰值均在第14天,第7d与第28d相比,无统计学差异。3.HE染色观察各组成骨状况与各组免疫组化结果相符。结论:三七总皂苷壳聚糖温控凝胶能加快牙槽窝的愈合进程,提升BMP-2的表达水平,抑制牙槽骨的吸收;三七总皂苷在中浓度(1.4mg/ml)应用时其作用性能优于高浓度组(2.8mg/ml)和低浓度组0.7mg/ml);壳聚糖温控凝胶具有一定的成骨诱导作用,但是单独应用效果并不明显。
[Abstract]:Objective: Because of the limitation of medicine, there are not many cases in the clinical work because of various local or systemic factors, and after the tooth extraction, the healing process of tooth extraction can directly affect the ability of the patient to wear the denture as soon as possible. and the shape and function of the partial tooth groove after the reconstruction of the tooth extraction socket have the influence on the retention and the stability of the dentures, the orthodontic movement of the adjacent teeth and the bone volume conditions of the artificial planting teeth, so that the process is the oral and maxillofacial surgery and the repair, Orthodontic and planting doctors are an important subject of common concern. The purpose of this study was to investigate the effect of chitosan-controlled gel on the healing of tooth extraction in rats, and to control the absorption of residual tooth and its effect on the expression of BMP-2. Methods: Forty-eight SD male rats (200 to 250g in body weight) were used to remove the dental crown from the gingival margin of the left and lower mandibular incisors for 9 days, 6 days and 3 days before the tooth extraction in the rat. Three dead in the model building phase. the remaining 45 rats were divided into five groups (high concentration group, medium concentration group, low concentration group, blank gel group and complete blank group) on the tenth day, and the left lower jaw and the left lower jaw were completely extracted, The high concentration (2. 8mg/ ml), medium concentration (1. 4mg/ ml), low concentration (0. 7mg/ ml), and 20ul of the blank gel and the blank control group were not put in the control group, respectively. Three groups of rats were randomly selected for cardiac perfusion fixation after 7, 14 and 28 days after operation. The relative length of the left and right mandibles of the rat was dissected, the relative length of the remaining tooth grooves in each group was measured, the pathological sections were made, the healing of the tooth extraction was observed by HE staining, and the expression of BMP-2 was detected by immunohistochemical staining, and the statistical analysis was carried out. Results: 1. The statistical results of the relative length of the remaining teeth in each group were as follows: no statistical difference was found between group 5 and group 5 on day 7 and day 28, and there was no statistical difference between the three drug-carrying groups and the blank gel group, the total blank group and the blank group, and the total blank group was all blank. The value of medium concentration group was higher than that of the high concentration group and the low concentration group, and there was no statistical difference between the high concentration group and the low concentration group, and the blank gel group was not statistically different from the complete blank group. There was no statistical difference in the relative length of the remaining teeth in the five groups at different time points in the same group, but the 28-day value was less than the 7th and 14th days, and there was a statistical difference. The results of the counting of BMP-2 positive cells in each group showed that there was a statistically significant difference between the three drug-carrying groups and the blank gel group, the total blank group ratio, the number of the three drug-carrying groups, and the total blank group. There were no significant differences in the number of concentration groups in the three groups compared with the high and low groups, and there was no statistical difference between the group of high concentration and the low concentration group at the 3 time points, and the blank group was not statistically different from the complete blank group. At different time points, the peak of the five-group positive cell counts was on the 14th day, and no statistical difference was found in the 7th day and the second 28d. Conclusion: The chitosan-chitosan temperature-controlled gel can accelerate the healing process of the alveolar fossa, improve the expression level of BMP-2, and inhibit the absorption of the alveolar bone. The effect of the total soap of the pseudo-ginseng is better than that of the high-concentration group (2.8mg/ ml) and the low-concentration group (0.7mg/ ml). The chitosan temperature-controlled gel has a certain bone-inducing effect, but the effect of the individual application is not obvious.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R782.1

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