Odanacatib抑制正畸牙齿移动后复发的实验研究
发布时间：2018-12-17 22:56
【摘要】：目的正畸复发牙移动的骨改建过程同时受成骨细胞和破骨细胞的持续作用。Odanacatib是一个高度选择性的组织蛋白酶K抑制剂,阻断破骨细胞的骨吸收效应,促进成骨,但其在正畸后复发领域的应用尚属空白。本实验从odanacatib调节破骨细胞功能,抑制移动牙复发的角度出发,通过建立大鼠正畸牙移动复发模型,在复发牙周围局部注射odanacatib,进一步了解正畸牙移动复发过程骨改建机制,分析外源性odanacatib对正畸牙移动复发距离、牙槽骨密度以及复发过程中TRAP、Cat K、IGF-Ⅰ表达的影响,探讨odanacatib是否具有促进牙齿稳定的作用,以期为正畸临床保持阶段提供有效的辅助方法。方法选用30只SPF级大鼠,建立种植支抗牙齿移动模型,60g力值近中移动右上颌第一磨牙,加力3周去除加力装置让其复发。随机分为实验组和对照组,每组15只。实验组于右上第一磨牙周围粘骨膜局部注射60μl、1.25μM odanacatib,每3天注射一次。对照组注射等量生理盐水。复发0天和2周时制取大鼠上颌牙列模型,测量右上颌第一、二磨牙间距离,计算复发率(复发率=复发距离/牙齿移动距离*100%)。复发2周后处死大鼠,立即截取包含右上颌3颗磨牙及其周围牙槽骨的上颌骨,进行Micro-CT扫描,测量右上颌第一磨牙根分叉处牙槽骨BMD和BVF值,分析牙槽骨骨小梁空间结构。扫描后制作组织切片,HE染色观察第一磨牙牙周组织形态学变化。TRAP特殊染色观察牙周破骨细胞数量的变化,免疫组织化学染色观察牙周组织Cat K和IGF-Ⅰ的表达,利用图像分析软件对其含量进行半定量分析。采用SPSS21.0统计软件进行分析。结果拆除矫治器后,2组大鼠第一磨牙均向远中移动造成复发,实验组复发率小于对照组,且实验组牙槽骨的BMD和BVF值高于对照组,差异有统计学意义。HE染色镜下观察可见实验组牙周膜纤维排列规则,近远中侧牙周膜间隙均匀,可见新骨形成。对照组牙周膜纤维排列比较规则,近远中侧牙周膜间隙比较均匀,可见新的类骨质填充。实验组TRAP阳性染色细胞的IOD值小于对照组,但无统计学差异;实验组Cat K的IOD值小于对照组,差异有统计学意义;实验组IGF-Ⅰ的表达高于对照组,差异有统计学意义。结论(1)局部注射odanacatib能有效地抑制破骨细胞Cat K的表达,但不减少破骨细胞的数量,对破骨细胞的存活没影响。(2)局部注射odanacatib减小大鼠正畸复发率,增加局部牙槽骨的BMD和BVF值。提示odanacatib在正畸牙复发过程中可以有效减少骨吸收,增加骨形成,抑制复发。(3)局部注射odanacatib增加牙周组织IGF-Ⅰ的分泌,提示odanacatib在正畸牙复发过程中保护胶原的降解,促进牙周组织改建,增强移动牙的稳定性。
[Abstract]:Objective the process of bone remodeling in orthodontic recurrent tooth movement is sustained by both osteoblasts and osteoclasts. Odanacatib is a highly selective cathepsin K inhibitor that blocks osteoclast resorption and promotes osteogenesis. But its application in the field of orthodontic recurrence is still blank. From the point of view of odanacatib regulating osteoclast function and inhibiting the recurrence of moving teeth, we established a rat model of orthodontic tooth movement and recurrence, and injected odanacatib, around the recurrent teeth to further understand the mechanism of bone remodeling in the process of orthodontic tooth movement and recurrence. To analyze the effects of exogenous odanacatib on the distance of orthodontic tooth movement and recurrence, alveolar bone mineral density and the expression of TRAP,Cat KF- 鈪，
本文编号：2384913
[Abstract]:Objective the process of bone remodeling in orthodontic recurrent tooth movement is sustained by both osteoblasts and osteoclasts. Odanacatib is a highly selective cathepsin K inhibitor that blocks osteoclast resorption and promotes osteogenesis. But its application in the field of orthodontic recurrence is still blank. From the point of view of odanacatib regulating osteoclast function and inhibiting the recurrence of moving teeth, we established a rat model of orthodontic tooth movement and recurrence, and injected odanacatib, around the recurrent teeth to further understand the mechanism of bone remodeling in the process of orthodontic tooth movement and recurrence. To analyze the effects of exogenous odanacatib on the distance of orthodontic tooth movement and recurrence, alveolar bone mineral density and the expression of TRAP,Cat KF- 鈪，
本文编号：2384913
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