L-选择素在口腔鳞状细胞癌组织和涎腺恶性肿瘤组织中的表达及其相关性的研究

发布时间：2018-12-19 11:20

【摘要】：目的：采用免疫组化法检测L-选择素（L-Selectin）在口腔鳞状细胞癌（OralSquamous Cell Carcinoma，OSCC）组织和涎腺恶性肿瘤（Salivary gland malignanttumor）组织中的表达情况，并初步探讨L-选择素与口腔鳞状细胞癌和涎腺恶性肿瘤的发生、发展和侵袭转移的相关性。方法：收集佳木斯大学第二附属医院·口腔医院病理科保存的手术切除的口腔鳞状细胞癌石蜡标本48例，涎腺恶性肿瘤石蜡标本48例，，另选择因口腔颌面部良性病变而切除的病变组织周围的正常口腔黏膜组织和正常涎腺组织各20例，分别作为对照组。采用链霉菌抗生物素蛋白-过氧化酶(SP)免疫组织化学方法检测L-选择素的表达情况，采用Concise Statistics，CS10.34统计软件进行统计学处理，探讨L-选择素的表达情况与口腔鳞状细胞癌和涎腺恶性肿瘤的各临床病理特征之间的关系。结果： 1.L-选择素在口腔鳞状细胞癌中的阳性表达率约为41.7%（20/48），明显高于其对照组的10%（2/20）（P0.05）。 2.L-选择素在涎腺恶性肿瘤中的阳性表达率约为68.8%（33/48），明显高于其对照组的10%（2/20）（P0.05）。 3.在口腔鳞状细胞癌组织中，L-选择素的表达与患者口腔鳞状细胞癌组织的分化程度、TNM分期和颈淋巴结转移有关；与患者的性别、年龄无关。 4.在涎腺恶性肿瘤组织中，L-选择素的表达与患者涎腺恶性肿瘤的颈淋巴结转移有关；与患者涎腺恶性肿瘤的组织分类，性别、年龄无关。 5.L-选择素在口腔鳞状细胞癌组织与涎腺恶性肿瘤组织中的表达显示，涎腺恶性肿瘤组织中的L-选择素阳性率明显高于其在鳞状细胞癌组织中的阳性率（P＜0.05）。结论：L-选择素在口腔鳞状细胞癌组织和涎腺恶性肿瘤组织中均存在着高表达；L-选择素在口腔鳞状细胞癌和涎腺恶性肿瘤的发生、发展和侵袭转移中起着重要作用；L-选择素有望作为口腔颌面部恶性肿瘤早期诊断的标志。
[Abstract]:Objective: to detect the expression of L-selectin (L-Selectin) in oral squamous cell carcinoma (OralSquamous Cell Carcinoma,OSCC) and salivary gland malignant tumor (Salivary gland malignanttumor) by immunohistochemical method. The relationship between L- selectin and the occurrence, development, invasion and metastasis of oral squamous cell carcinoma and salivary gland malignant tumor was studied. Methods: the paraffin specimens of 48 cases of oral squamous cell carcinoma and 48 cases of malignant tumors of salivary gland were collected from Department of Stomatology, second affiliated Hospital of Jiamusi University. In addition, 20 cases of normal oral mucosa and 20 cases of normal salivary gland were selected as control group. The expression of L-selectin was detected by streptomyces biotin-peroxidase (SP) immunohistochemical method. The expression of L-selectin was analyzed by Concise Statistics,CS10.34 software. To investigate the relationship between the expression of L-selectin and the clinicopathological features of oral squamous cell carcinoma and salivary gland malignant tumor. Results: 1. The positive expression rate of L- selectin in oral squamous cell carcinoma was 41.7% (20 / 48), which was significantly higher than that in control group (10%, 2 / 20) (P0.05). 2. The positive expression rate of L- selectin in salivary gland malignant tumor was 68.8% (33 / 48), which was significantly higher than that in control group (10%, 2 / 20) (P0.05). 3. In oral squamous cell carcinoma (OSCC), the expression of L- selectin was related to the degree of differentiation, TNM stage and cervical lymph node metastasis, but not to sex and age. 4. The expression of L- selectin was related to cervical lymph node metastasis in salivary gland malignant tumors, but not to the classification, sex and age of salivary gland malignant tumors. 5. The expression of L- selectin in oral squamous cell carcinoma and salivary gland malignant tumor showed that the positive rate of L- selectin in salivary gland malignant tumor was significantly higher than that in squamous cell carcinoma (P < 0.05). Conclusion: L- selectin is highly expressed in oral squamous cell carcinoma and salivary gland malignant tumor, and it plays an important role in the occurrence, development, invasion and metastasis of oral squamous cell carcinoma and salivary gland malignant tumor. L-selectin is expected to be a marker for early diagnosis of oral and maxillofacial malignant tumors.
【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.8

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