牙周基础治疗对肥胖大鼠实验性牙周炎及代谢水平的影响
发布时间：2019-01-02 14:04
【摘要】：目的探讨牙周干预治疗肥胖合并牙周炎的大鼠,对大鼠牙周炎的控制、胰岛素抵抗及脂代谢水平的影响。方法将SD大鼠随机分为正常组(C组)、肥胖组(O组)、牙周炎合并肥胖组(P组)和牙周治疗组(T组)。P组和T组大鼠行上颌第2磨牙牙周结扎,T组大鼠做基础牙周治疗,所有大鼠24周龄处死。眼眶静脉取血检测血脂四项,胰岛素和血糖,计算胰岛素抵抗(HOMA稳态模型胰岛素抵抗值,HOMA-IR)。取大鼠颌骨及各脏器行病理观察;实时定量PCR法检测肝脏中胰岛素受体底物-1(IRS-1),胰岛素受体底物-2(IRS-2)的表达。结果 P组与单纯肥胖O组比较:HOMA-IR、LDL-C值均出现升高;而IRS-1、IRS-2表达及HDL-C值则低于肥胖组。T组与P组比较,大鼠的胰岛素抵抗HOMA-IR值下降(P0.05);IRS-1及IRS-2的mRNA表达均有增高(P0.05),TG值变化不明显(P0.05),HDL-C升高,LDL-C及TC值出现下降(P0.05)。病理学观察:T组大鼠颌骨标本切片中的炎细胞浸润情况出现好转。P组大鼠颌骨破坏最严重。肝脏切片比较,P组大鼠的肝细胞中肝脂变最为广泛,炎症浸润最严重。结论牙周的炎症可下调IRS-1,IRS-2的表达,加重肥胖大鼠的胰岛素抵抗和血脂代谢紊乱。牙周治疗对于改善肥胖大鼠胰岛素抵抗的情况,减轻血脂代谢紊乱有一定的影响。
[Abstract]:Objective to investigate the effects of periodontal intervention on periodontitis control, insulin resistance and lipid metabolism in obese rats with periodontitis. Methods SD rats were randomly divided into normal group (C group), obese group (O group), periodontitis combined with obesity group (P group) and periodontal treatment group (T group). P and T group rats) were treated with maxillary second molar periodontal ligation. Rats in group T were treated with basic periodontal therapy and all rats were killed at 24 weeks old. Serum lipids, insulin and blood glucose were measured by orbital vein blood collection. Insulin resistance (HOMA steady-state model insulin resistance, HOMA-IR) was calculated. The expression of insulin receptor substrate-1 (IRS-1) and insulin receptor substrate-2 (IRS-2) in the liver were detected by real-time quantitative PCR. Results compared with simple obesity group O, the HOMA-IR,LDL-C value of P group was higher than that of simple obesity O group. The expression of IRS-1,IRS-2 and the value of HDL-C were lower in group T than those in group P. the HOMA-IR value of insulin resistance in group T was lower than that in group P (P0.05). The mRNA expression of IRS-1 and IRS-2 were increased (P0.05), TG value was not significantly changed (P0.05), HDL-C increased, LDL-C and TC values decreased (P0.05). Pathological observation: the infiltration of inflammatory cells in group T was improved, and the destruction of jaw was the most serious in group P. In group P, liver lipid was the most extensive and inflammatory infiltration was the most serious. Conclusion periodontal inflammation can down-regulate the expression of IRS-1,IRS-2 and aggravate insulin resistance and dyslipidemia in obese rats. Periodontal therapy has a certain effect on improving insulin resistance and reducing lipid metabolism disorder in obese rats.
【作者单位】： 南方医科大学口腔医院//广东省口腔医院;中国科学院大学存济医学院;
【基金】：国家自然科学基金(81300890) 广东省医学科学技术研究基金(A2014110)~~
【分类号】：R781.42
本文编号：2398589
[Abstract]:Objective to investigate the effects of periodontal intervention on periodontitis control, insulin resistance and lipid metabolism in obese rats with periodontitis. Methods SD rats were randomly divided into normal group (C group), obese group (O group), periodontitis combined with obesity group (P group) and periodontal treatment group (T group). P and T group rats) were treated with maxillary second molar periodontal ligation. Rats in group T were treated with basic periodontal therapy and all rats were killed at 24 weeks old. Serum lipids, insulin and blood glucose were measured by orbital vein blood collection. Insulin resistance (HOMA steady-state model insulin resistance, HOMA-IR) was calculated. The expression of insulin receptor substrate-1 (IRS-1) and insulin receptor substrate-2 (IRS-2) in the liver were detected by real-time quantitative PCR. Results compared with simple obesity group O, the HOMA-IR,LDL-C value of P group was higher than that of simple obesity O group. The expression of IRS-1,IRS-2 and the value of HDL-C were lower in group T than those in group P. the HOMA-IR value of insulin resistance in group T was lower than that in group P (P0.05). The mRNA expression of IRS-1 and IRS-2 were increased (P0.05), TG value was not significantly changed (P0.05), HDL-C increased, LDL-C and TC values decreased (P0.05). Pathological observation: the infiltration of inflammatory cells in group T was improved, and the destruction of jaw was the most serious in group P. In group P, liver lipid was the most extensive and inflammatory infiltration was the most serious. Conclusion periodontal inflammation can down-regulate the expression of IRS-1,IRS-2 and aggravate insulin resistance and dyslipidemia in obese rats. Periodontal therapy has a certain effect on improving insulin resistance and reducing lipid metabolism disorder in obese rats.
【作者单位】： 南方医科大学口腔医院//广东省口腔医院;中国科学院大学存济医学院;
【基金】：国家自然科学基金(81300890) 广东省医学科学技术研究基金(A2014110)~~
【分类号】：R781.42
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相关博士学位论文 前1条
1 倪佳;牙周炎影响肥胖大鼠胰岛素抵抗机制的初步研究[D];南方医科大学;2012年
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