高糖对人牙周膜细胞骨向分化的影响
发布时间：2019-01-12 19:19
【摘要】：目的：观察体外高糖培养环境对人牙周膜细胞(periodontal ligament cells, PDLCs)增殖及骨向分化的影响。 方法：原代培养人牙周膜细胞,取4-6代细胞用于实验。运用MTT法检测正常组和高糖组牙周膜细胞在不同培养环境中的增殖活性。细胞在低糖和高糖DMEM配制的正常组矿化液和高糖组矿化液培养基(50mg/ml维生素C,10mMβ-磷酸甘油钠和100nM地塞米松)中培养后,于第1,7,14,21和28天运用茜素红染色法观察矿化结节形成,以及观察不同培养环境下PDLCs碱性磷酸酶(Alkaline Phosphatase, ALP)活性和Ⅰ型胶原蛋白的表达。 结果：高糖能抑制牙周膜细胞的增殖；高糖矿化组和正常矿化组之间,矿化结节形成有明显差异；高糖矿化组和正常矿化组ALP活性和Ⅰ型胶原蛋白表达有差异。 结论：高糖对牙周膜细胞增殖和骨向分化有抑制作用,进一步解释了糖尿病牙周炎患者病情严重且预后延迟的机制。
[Abstract]:Aim: to observe the effect of high glucose culture on (periodontal ligament cells, PDLCs) proliferation and bone differentiation of human periodontal ligament cells in vitro. Methods: human periodontal ligament cells were cultured in primary culture. The proliferative activity of periodontal ligament cells in normal and high glucose groups was detected by MTT method. The cells were cultured in 50mg/ml Vitamin C, 10mM 尾 -Glycerol Phosphate and 100nM Dexamethasone medium (50mg/ml Vitamin C, 10mM 尾 -Glycerol Phosphate and 100nM Dexamethasone) prepared with low glucose and high glucose DMEM. On day 21 and 28, alizarin red staining was used to observe the formation of mineralized nodules, the activity of PDLCs alkaline phosphatase (Alkaline Phosphatase, ALP) and the expression of type I collagen under different culture conditions. Results: high glucose could inhibit the proliferation of periodontal ligament cells, there were significant differences in the formation of mineralized nodules between high glucose mineralization group and normal mineralization group, and there were differences in ALP activity and type I collagen expression between high glucose mineralization group and normal mineralization group. Conclusion: high glucose can inhibit the proliferation and bone differentiation of periodontal ligament cells, which further explains the mechanism of severe disease and delayed prognosis in patients with diabetic periodontitis.
【学位授予单位】：武汉大学
【学位级别】：博士
【学位授予年份】：2014
【分类号】：R781.4
[Abstract]:Aim: to observe the effect of high glucose culture on (periodontal ligament cells, PDLCs) proliferation and bone differentiation of human periodontal ligament cells in vitro. Methods: human periodontal ligament cells were cultured in primary culture. The proliferative activity of periodontal ligament cells in normal and high glucose groups was detected by MTT method. The cells were cultured in 50mg/ml Vitamin C, 10mM 尾 -Glycerol Phosphate and 100nM Dexamethasone medium (50mg/ml Vitamin C, 10mM 尾 -Glycerol Phosphate and 100nM Dexamethasone) prepared with low glucose and high glucose DMEM. On day 21 and 28, alizarin red staining was used to observe the formation of mineralized nodules, the activity of PDLCs alkaline phosphatase (Alkaline Phosphatase, ALP) and the expression of type I collagen under different culture conditions. Results: high glucose could inhibit the proliferation of periodontal ligament cells, there were significant differences in the formation of mineralized nodules between high glucose mineralization group and normal mineralization group, and there were differences in ALP activity and type I collagen expression between high glucose mineralization group and normal mineralization group. Conclusion: high glucose can inhibit the proliferation and bone differentiation of periodontal ligament cells, which further explains the mechanism of severe disease and delayed prognosis in patients with diabetic periodontitis.
【学位授予单位】：武汉大学
【学位级别】：博士
【学位授予年份】：2014
【分类号】：R781.4
【参考文献】
相关期刊论文 前5条
1 唐q 琪;钟泉;李大兰;姚丽艳;李艳芬;闫福华;;不同培养基对人牙周膜细胞生物学行为的影响[J];口腔生物医学;2011年03期
2 韩R，
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