纯钛微弧氧化—多巴胺—环RGD涂层的细胞相容性研究

发布时间：2019-01-17 06:40

【摘要】：目的:探讨纯钛种植体经多巴胺修饰后加载环型RGD多肽复合涂层对成骨细胞生物行为的影响,比较不同纯钛表面处理后体外生物相容性的异同。方法:借助微弧氧化技术和化学偶联技术在纯钛表面构建陶瓷氧化膜-聚多巴胺-环RGD活性涂层,本实验分为三组,微弧氧化组为M组;微弧氧化加载多巴胺组为P组;微弧氧化加载多巴胺接枝环肽组为R组。采用扫描电镜(SEM)观察钛片表面形貌;电子能谱(XPS)分析三组钛片表面元素组成成分;常规条件下培养成骨细胞,CCK-8检测成骨细胞在不同组别、不同时间点的黏附及增殖能力;荧光显微镜观察成骨细胞24小时后在三组涂层表面的细胞形态。结果:扫描电镜显示表面形貌的改变,能谱分析显示RGD环肽涂层加载成功,CCK8检测结果显示细胞的增殖及粘附,P0.05有统计学意义,且R组P组M组,荧光显微镜观察R组、P组均较M组更利于细胞增殖和黏附,但R组更利于细胞铺展,且细胞间联系紧密。结论:三组实验中P组和R组都能有效促进种植体的骨结合,其中R组效果更佳,对于种植体的涂层改进有一定的临床应用意义。
[Abstract]:Aim: to investigate the effects of dopamine modified RGD polypeptide composite coating on the biological behavior of osteoblasts and compare the biocompatibility of titanium implants with different titanium surface treatments in vitro. Methods: ceramic oxide film polydopamine cyclic RGD active coating was constructed on the surface of pure titanium by means of microarc oxidation and chemical coupling technique. The experiment was divided into three groups: group M (group M), group P (group B) loaded by micro-arc oxidation (group P); The dopamine grafted cyclic peptide group was treated with microarc oxidation. Scanning electron microscope (SEM) was used to observe the surface morphology of titanium sheet, and (XPS) was used to analyze the composition of elements on the surface of three groups of titanium sheets. Osteoblasts were cultured under conventional conditions. CCK-8 was used to detect the adhesion and proliferation of osteoblasts in different groups and at different time points. The morphology of osteoblasts on the surface of the three groups was observed by fluorescence microscope after 24 hours. Results: scanning electron microscope showed the change of surface morphology, energy dispersive analysis showed that RGD cyclic peptide coating was loaded successfully, CCK8 test showed cell proliferation and adhesion, P 0.05, and group R P M, fluorescence microscope observation R group. Group P was more conducive to cell proliferation and adhesion than group M, but group R was more conducive to cell spreading and close relationship between cells. Conclusion: both group P and group R can promote the osseointegration of implants effectively in the three groups, and the effect of group R is better than that of group R, which has a certain clinical significance for the improvement of implant coating.
【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.1

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