淫羊藿对大鼠牙移动后保持阶段牙周组织中BMP-2表达的影响

发布时间：2019-02-16 02:32

【摘要】：目的:通过建立大鼠正畸牙移动后保持阶段的实验模型,模仿临床上正畸患者牙移动后的保持,在实验动物口腔内局部注射药物淫羊藿,观察牙周组织中的BMP-2的表达变化,评价中药淫羊藿在牙齿矫治之后抑制其复发方面的作用,探究淫羊藿在促进牙周组织改建过程中的作用机理,为临床中正畸治疗后减短保持阶段的时间以及使牙齿尽快的稳定在理想的位置上提供了一种药物辅助措施。方法:40只wistar大鼠分为实验组A组、B组C组和对照组,每组10只大鼠。在牙移动模型去除之前的1d,实验组分别注射浓度为5ug/ml、10ug/ml、15ug/ml,的淫羊藿溶液,对照组同时注射同浓度,同剂量的生理盐水,实验组和对照组都是每过2d注射一次,各组大鼠在装置去除之后的第七天分别处死5只,第二十八天再分别处死5只,通过测量上颌第一磨牙的复发距离,HE染色观测法以及免疫组织化学染色的方法,分析淫羊藿对大鼠牙移动后保持阶段牙周组织的改建和BMP-2的表达影响。结果:装置去除后第7天,对照组的复发距离大于实验组各组复发距离分别为0.130mm、0.148mm、0.107mm。实验组成骨细胞数量比对照组分别增加0.32、0.44、0.21,实验组BMP-2表达平均光密度值比对照组分别增加0.019、0.023、0.014,实验组与对照组比较有显著差异(P0.05)。装置去除后第28天,对照组的复发距离大于实验组复发距离分别为,0.105mm、0.151mm、0.077mm。实验组成骨细胞数量比对照组分别增加0.38、0.63、0.01,实验组BMP-2表达平均光密度值比对照组分别增加0.030、0.045、0.023,实验组和对照组两组比较有显著差异(P0.05)。结论:局部注射中药淫羊藿可以增加大鼠实验性牙移动后保持阶段中牙周组织B MP-2的表达,对骨细胞的生成有促进作用。
[Abstract]:Objective: to establish an experimental model of rat orthodontic tooth retention after tooth movement, and to observe the expression of BMP-2 in periodontal tissues by injecting epimedium into the oral cavity of experimental animals, mimicking the retention of orthodontic teeth after tooth movement. To evaluate the role of Herba Epimedii in inhibiting the recurrence of Epimedium after tooth treatment, and to explore the mechanism of Epimedium in promoting periodontal tissue remodeling. It provides a drug assistant measure for reducing the duration of maintenance after orthodontic treatment and keeping teeth stable in ideal position as soon as possible. Methods: 40 wistar rats were divided into three groups: experimental group A, group B, group C and control group, with 10 rats in each group. One day before the tooth movement model was removed, the experimental group was injected with epimedium solution with a concentration of 5 g / ml 10 g / ml and 15 g / ml, respectively, while the control group was injected with the same concentration and same dose of normal saline at the same time. The experimental group and the control group were injected every 2 days. The rats in each group were killed on the seventh day after the device was removed, and 5 rats were killed on the 28 day. The recurrence distance of the maxillary first molar was measured by measuring the recurrence distance of the maxillary first molar. The effects of Epimedium Epimedii on periodontal remodeling and expression of BMP-2 in rats after tooth movement were analyzed by HE staining and immunohistochemical staining. Results: on the 7th day after removal of the device, the recurrence distance of the control group was 0.130 mm / 0.148 mm / 0.107 mm, respectively. Compared with the control group, the number of osteoblasts in the experimental group increased by 0.32, 0.44 and 0.21, and the average optical density of BMP-2 expression in the experimental group increased by 0.019, 0.023 and 0.014, respectively. There was a significant difference between the experimental group and the control group (P0.05). On the 28th day after removal of the device, the recurrence distance of the control group was greater than that of the experimental group, 0.105mm / 0.151mm / 0.077mm. Compared with the control group, the number of osteoblasts in the experimental group increased by 0.38 ~ 0.63% and the average optical density of BMP-2 expression in the experimental group was 0.030 ~ 0.045 ~ 0.023, respectively. There was a significant difference between the experimental group and the control group (P0.05). Conclusion: local injection of Herba Epimedii can increase the expression of B MP-2 in periodontal tissue and promote the formation of osteocytes during the period of experimental tooth movement in rats.
【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.5

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