根管-根尖周复合体体外模型的建立
发布时间：2019-02-16 18:40
【摘要】：目的建立根管-根尖周组织复合体体外模型,以此探索感染根管形成根尖周炎及根尖生物膜的可能机制。方法将因正畸减数拔除的健康单根前磨牙密封于盛有LB固体培养基的无菌小瓶内,使牙根的根尖1/3置于培养基中,制备成根管-根尖周复合体体外模型25个。建模后1d抽取5个模型通过PCR技术检测根尖周组织有无细菌。将余下的20个模型分为对照组(n=10)及实验组(n=10),实验组作开髓处理,对照组不做任何处理,自然放置于空气中,经21d检测根管内和根尖周有无细菌,及终点显色法检测根尖周内毒素浓度。结果第21天,实验组根管内检测到细菌,对照组根管内未检测到细菌,而所有模型根尖周均检测到细菌;对照组根尖周内毒素含量平均为(8.913±0.614)EU/mL,实验组为(10.525±0.981)EU/mL,两组比较差异有统计学意义(P0.01)。结论通过上述方法成功建立根管-根尖周复合体体外模型。未对根管做处理时,感染根管内的细菌不会到达根尖周,而感染根管内的细菌首先是通过分泌的内毒素等致病因子到达根尖周引起根尖周炎。
[Abstract]:Objective to establish a model of root canal-periapical tissue complex in vitro to explore the possible mechanism of apical periodontitis and apical biofilm caused by infection of root canals. Methods the healthy premolars extracted by orthodontic subtraction were sealed in a sterile flask containing LB solid medium. The root tip of the root was placed in the medium for 25 models of root canal-periapical complex in vitro. On the first day after modeling, 5 models were selected to detect the presence of bacteria in periapical tissue by PCR technique. The remaining 20 models were divided into two groups: control group (n = 10) and experimental group (n = 10). The experimental group was treated with open pulp, and the control group was placed in the air without any treatment. After 21 days, the root canal and the periapical zone were detected for the presence of bacteria in the root canal and periapical zone. The concentration of endotoxin in periapical region was determined by the end point colorimetric method. Results on the 21st day, bacteria were detected in the root canals in the experimental group, but not in the root canals in the control group. The average periapical endotoxin content in the control group was (8.913 卤0.614) EU/mL, and (10.525 卤0.981) EU/mL, in the experimental group. There was significant difference between the two groups (P0.01). Conclusion the in vitro model of root canal-periapical complex was successfully established by the above methods. When the root canal is not treated, the bacteria in the infected root canal will not reach the periapical zone, but the bacteria in the infected root canal will first reach the periapical periodontitis by secreting pathogenic factors such as endotoxin.
【作者单位】： 重庆医科大学附属口腔医院牙体牙髓科/口腔疾病与生物医学重庆市重点实验室;
【基金】：重庆市卫生局医学科研重点项目(2012-1-053)
【分类号】：R781.34
[Abstract]:Objective to establish a model of root canal-periapical tissue complex in vitro to explore the possible mechanism of apical periodontitis and apical biofilm caused by infection of root canals. Methods the healthy premolars extracted by orthodontic subtraction were sealed in a sterile flask containing LB solid medium. The root tip of the root was placed in the medium for 25 models of root canal-periapical complex in vitro. On the first day after modeling, 5 models were selected to detect the presence of bacteria in periapical tissue by PCR technique. The remaining 20 models were divided into two groups: control group (n = 10) and experimental group (n = 10). The experimental group was treated with open pulp, and the control group was placed in the air without any treatment. After 21 days, the root canal and the periapical zone were detected for the presence of bacteria in the root canal and periapical zone. The concentration of endotoxin in periapical region was determined by the end point colorimetric method. Results on the 21st day, bacteria were detected in the root canals in the experimental group, but not in the root canals in the control group. The average periapical endotoxin content in the control group was (8.913 卤0.614) EU/mL, and (10.525 卤0.981) EU/mL, in the experimental group. There was significant difference between the two groups (P0.01). Conclusion the in vitro model of root canal-periapical complex was successfully established by the above methods. When the root canal is not treated, the bacteria in the infected root canal will not reach the periapical zone, but the bacteria in the infected root canal will first reach the periapical periodontitis by secreting pathogenic factors such as endotoxin.
【作者单位】： 重庆医科大学附属口腔医院牙体牙髓科/口腔疾病与生物医学重庆市重点实验室;
【基金】：重庆市卫生局医学科研重点项目(2012-1-053)
【分类号】：R781.34
【参考文献】
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1 庄沛林;高燕;凌均h，
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