重组人粒细胞-巨噬细胞集落刺激因子对兔牙龈创伤愈合的促进作用

发布时间：2019-03-06 14:09

【摘要】：目的:探讨重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)对于牙龈创伤修复的影响,为其在口腔牙周领域中的应用奠定基础。方法:16只健康大耳白兔随机分为实验组和对照组,每组8只。采用手术刀切除上下切牙唇侧牙龈2mm。术后实验组每日3次创面涂抹rhGM-CSF,对照组以生理盐水替代。于术后3、7、11和15d各处死动物2只,HE染色观察镜下上皮及上皮下结缔组织的结构、分布,各种创伤修复细胞的变化,PCNA染色后观察阳性染色上皮细胞和成纤维细胞数目。结果:形态学观察,第3天2组均表现出炎细胞浸润,但实验组浸润数目更多;第7天2组均可见到新生血管、成纤维细胞和胶原纤维,实验组新生血管化的程度较为显著;第11天2组出现大量增殖的成纤维细胞,但实验组和程度更广泛,至第15天时2组的创伤均已基本恢复正常,组间差异不明显。PCNA染色,随着时间的推移,增殖的上皮细胞数目逐渐增加,至第15天时达到高峰,增殖的成纤维细胞数目从第3天起开始增加,至第11天达到高峰,第15天则明显降低为最低值。实验组增殖的上皮细胞数量在第3和11天显著高于对照组(P0.01),增殖的成纤维细胞数量在第11和15天明显高于对照组(P0.05),其余时间点组间比较差异均无统计学意义(P0.05)。结论:rhGM-CSF可以促进牙龈组织中炎细胞的浸润、血管的新生、上皮细胞和成纤维细胞的增殖,rhGM-CSF可以促进牙龈组织创伤愈合。
[Abstract]:Aim: to investigate the effect of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on gingival wound repair and to lay a foundation for its application in oral periodontium. Methods: sixteen healthy white rabbits were randomly divided into experimental group (n = 8) and control group (n = 8). The labial gingiva of upper and lower incisors was resected with scalpel 2 mm. RhGM-CSF, was applied to the wound of the experimental group 3 times a day after operation. The control group was replaced with saline. Two animals were killed at 3, 7, 11 and 15 days after operation. The structure and distribution of epithelial and subcutaneous connective tissue under microscope were observed by HE staining, and the changes of various wound repair cells were observed. After PCNA staining, the number of positive staining epithelial cells and fibroblasts was observed. Results: morphological observation showed that inflammatory cell infiltration was observed in both groups on the 3rd day, but the number of infiltration in the experimental group was more than that in the experimental group on the 7th day, neovascularization, fibroblasts and collagen fibers were found in both groups, and the degree of neovascularization in the experimental group was more significant than that in the experimental group. On the 11th day, a large number of proliferative fibroblasts appeared in the two groups, but the experimental group and the degree were more extensive. By the 15th day, the trauma of the two groups had basically returned to normal, and there was no significant difference between the two groups. PCNA staining, with the passage of time, showed no significant difference between the two groups. The number of proliferative epithelial cells increased gradually, reached the peak at the 15th day, and the number of proliferative fibroblasts increased from the 3rd day to the 11th day, and decreased to the lowest value on the 15th day. The number of proliferative epithelial cells in the experimental group was significantly higher than that in the control group on the 3rd and 11th days (P0.01), and the number of proliferative fibroblasts in the experimental group was significantly higher than that in the control group on the 11th and 15th days (P0.05). There was no significant difference between the other time points (P0.05). Conclusion: rhGM-CSF can promote the infiltration of inflammatory cells, angiogenesis, proliferation of epithelial cells and fibroblasts in gingival tissue. RhGM-CSF can promote wound healing of gingival tissue.
【作者单位】：吉林大学口腔医院牙周病科;福建省厦门市第二医院口腔科;吉林大学口腔医院动物实验室;
【基金】：吉林省科技厅重点项目资助课题(20090437)
【分类号】：R780.2

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