A-NK细胞抗口腔舌鳞状细胞癌的实验研究

发布时间：2019-03-11 14:54

【摘要】：目的观察黏附性自然杀伤(A-NK)细胞体外生长与增殖能力,探讨其治疗裸鼠舌鳞状细胞癌移植瘤的效果及其作用机制,为临床治疗鳞癌提供理论依据。方法抽取健康人外周血,分选出A-NK细胞和非黏附性自然杀伤(NA-NK)细胞体外培养,镜下观察细胞生长情况。建立裸鼠舌鳞癌皮下移植瘤模型,随机分成3组,每组7只小鼠,分别瘤旁注射生理盐水、A-NK细胞和NA-NK细胞,观察肿瘤体积变化,33 d后,处死裸鼠,剥离移植瘤,称瘤质量,绘制肿瘤生长曲线。结果镜下观察,A-NK细胞在第15天达到增殖高峰,NA-NK细胞于第12天达到增殖高峰。培养3周后,A-NK细胞共增加39.33倍,NA-NK细胞仅增加16.33倍。生理盐水组裸鼠口腔舌鳞癌移植瘤体积大于NA-NK细胞组和A-NK细胞组,NA-NK细胞组大于A-NK细胞组,3组裸鼠肿瘤体积在组间、时间和交互效应上差异均有统计学意义(P0.01)。第33天,生理盐水组裸鼠瘤质量大于NA-NK细胞组和A-NK细胞组,NA-NK细胞组大于A-NK细胞组(P0.05)。结论 A-NK细胞和NA-NK细胞均可在裸鼠体内诱导明显的抗肿瘤效应,且A-NK的抑瘤作用大于NA-NK细胞。
[Abstract]:Aim to observe the growth and proliferation of adhesive natural killer (A-NK) cells in vitro, and to explore the therapeutic effect and mechanism of adhensive natural killer cells in nude mice with tongue squamous cell carcinoma (LSCC), so as to provide a theoretical basis for clinical treatment of squamous cell carcinoma (SCC). Methods A-NK cells and non-adhesive natural killer (NA-NK) cells were selected from the peripheral blood of healthy people and cultured in vitro. The growth of the cells was observed under microscope. The model of subcutaneous transplantation of tongue squamous cell carcinoma in nude mice was established. Seven mice in each group were randomly divided into 3 groups. Normal saline, A-NK cells and NA-NK cells were injected into the tumor side to observe the change of tumor volume. 33 days later, nude mice were killed and transplanted tumor was exfoliated. Weigh the mass of the tumor and draw the growth curve of the tumor. Results under microscope, A-NK cells reached the peak of proliferation on the 15th day and NA-NK cells reached the proliferation peak on the 12th day. After 3 weeks of culture, A-NK cells increased 39.33-fold and NA-NK cells only 16.33-fold. The transplanted tumor volume of oral tongue squamous cell carcinoma in saline group was larger than that of NA-NK cell group and A-NK cell group, and that of NA-NK cell group was larger than that of A-NK cell group, and the tumor volume of three groups was between groups. There were significant differences in time and interaction (P0.01). On the 33rd day, the tumor mass of nude mice in saline group was larger than that in NA-NK cell group and A-NK cell group, and that in NA-NK cell group was higher than that in A-NK cell group (P0.05). Conclusion both A-NK cells and NA-NK cells can induce obvious antitumor effect in nude mice, and the antitumor effect of A-NK is greater than that of NA-NK cells.
【作者单位】：新疆医科大学附属中医医院口腔科;
【基金】：自治区科技支疆项目(201191158)
【分类号】：R739.8

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