具核梭杆菌对牙龈卟啉单胞菌或伴放线聚集杆菌诱导多形核白细胞活性氧生成的影响

发布时间：2019-03-20 15:20

【摘要】：目的:探讨与具核梭杆菌(F.nucleatum,Fn)共同感染时,牙龈卟啉单胞菌(P.gingivalis,Pg)或伴放线聚集杆菌(A.actinomycetemcomitans,Aa)对多形核白细胞(PMNs)产生活性氧(ROS)的影响。方法:取健康人抗凝外周血,经Percoll分离后取PMNs,将分离的PMNs与2’,7’-二氯荧光素二乙酸酯(DCFH-DA)混合,于37℃的水浴箱中孵育15min,然后分别向管中加入A组和B组细菌(细菌分组:A1,Pg;A2,Aa;A3,Fn;B1,Pg+Fn;B2,Aa+Fn;B3,Pg+Aa;B4,Pg+Aa+Fn;B5,Aa+Pg+Fn),充分混合后于37℃孵育1h和4h,流式细胞术(Flow Cytometry,FCM)检测PMNs产生ROS的量。结果:不同牙周致病菌与分离的PMNs在37℃作用1h,PMNs生成ROS的量分别是A1-16.52;A2-18.74;A3-19.59;B1-17.60;B2-20.09,其中B1A1;B2A2,差异显著(P0.05)。不同微生物与分离的PMNs在37℃作用4h,PMNs生成ROS的量分别是A1-176.05;A2-203.19;A3-230.36;B1-259.08;B2-274.89,其中B1A1;B2A2,差异显著(P0.05)。结论:与Fn共聚后,Pg或Aa诱导PMNs产生ROS的能力被增强。
[Abstract]:Objective: To study the effect of P. gingvalis (Pg) or A. actinifera comitans (Aa) on the production of reactive oxygen (ROS) in polymorphonuclear leukocytes (PMNs) with the co-infection of F. nafatum, Fn. Methods: The peripheral blood of healthy people was collected by Percoll, and the isolated PMNs were mixed with 2 ',7'-dichlorofluorescein diacetate (DCFH-DA) and incubated in a water bath box at 37 鈩，

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