不同粗化处理对超细晶纯钛表面性能的影响

发布时间：2019-03-22 15:34

【摘要】：目的:研究不同粗化处理对超细晶纯钛表面性能及成骨细胞黏附和增殖的影响。方法:将超细晶纯钛棒切割为直径7mm、厚度2 mm的试件,按不同喷砂压力(0.2~0.8 MPa)分组,对其表面进行喷砂酸蚀处理,对照组为普通纯钛。通过表面形貌、粗糙度、亲水性研究材料的表面性能,然后将大鼠胚胎成骨细胞(MC3T3-E1)接种到各组钛片表面,观察细胞初期黏附形态,测定其增殖密度。结果:超细晶纯钛粗化处理后,表面呈现出由喷砂和酸蚀所形成的大小不同的弹坑状双层结构。随着喷砂压力增大,超细晶纯钛表面坑孔直径和粗糙度逐渐增大,但二者均小于普通纯钛对照组(P0.05)。超细晶纯钛亲水性也随喷砂压力变化而改变,当喷砂压力为0.6 MPa时表现出最佳表面亲水性能。接种细胞后,实验组细胞初期黏附形态优于对照组,当喷砂压力为0.6 MPa时细胞增殖密度最大。结论:对超细晶纯钛喷砂酸蚀处理,喷砂压力为0.6 MPa时,材料表面形貌优于普通纯钛,粗糙度适宜,亲水性良好,更有利于细胞黏附和增殖。
[Abstract]:Objective: To study the effect of different coarsening treatment on the surface properties of ultra-fine crystalline titanium and the adhesion and proliferation of osteoblast. Method: The ultra-fine crystal pure titanium rod was cut into a test piece with a diameter of 7 mm and a thickness of 2 mm, and the surface of the test piece was subjected to sand-blast acid etching treatment on the surface of the test piece with different blast pressure (0.2-0.8MPa), and the control group was ordinary pure titanium. The surface properties of the material were studied by surface morphology, roughness and hydrophilicity, and then the rat embryonic osteoblasts (MC3T3-E1) were inoculated to the surface of each group of titanium tablets, and the initial adhesion morphology of the cells was observed and the proliferation density was determined. Results: After the fine-grained pure titanium roughening treatment, the surface exhibited a crater-like double-layer structure, which was formed by sandblasting and acid etching. With the increase of the blast pressure, the diameter and the roughness of the surface pit of the ultra-fine crystal were gradually increased, but both were less than that of the normal pure titanium control group (P0.05). The hydrophilicity of the ultra-fine crystal is also changed with the change of the sand-blasting pressure, and the best surface hydrophilic property is exhibited when the sand-blasting pressure is 0.6 MPa. After the cells were inoculated, the initial adhesion of the experimental group was superior to that of the control group, and the cell proliferation density was the most when the blast pressure was 0.6 MPa. Conclusion: The surface morphology of the material is superior to that of the common pure titanium, the roughness is suitable, the hydrophilicity is good, and the cell adhesion and proliferation are more favorable.
【作者单位】：军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔医学重点实验室第四军医大学口腔医院修复科;
【基金】：国家自然科学基金面上项目(编号:11672327) 陕西省社会发展科技攻关项目(编号:2016SF-155)
【分类号】：R783.1

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