Beyond祛氟剂和唾液再矿化对恒牙釉质影响的实验研究
发布时间：2019-05-21 23:51
【摘要】：目的： 通过冷光美白联合祛氟剂及唾液处理恒牙釉质表面物理性能和形态结构的观察分析，研究健康恒牙表层釉质显微硬度、成分、结构受祛氟剂和唾液再矿化的影响，为临床应用祛氟剂提供理论依据。 实验方法： 选择近期因需正畸治疗而拔除的健康上颌前磨牙30颗，沿近远中向和颊舌向切割牙冠成4部分，得到120个样本。选择测试面平行于水平面的100个样本，随机分成10组。空白对照组，Beyond美白凝胶组（即刻组，一周组，两周组），Beyond美白凝胶+脱色剂（Ｉ液）（即刻组，一周组，两周组），Beyond美白凝胶+脱色剂（Ｉ液）和再矿化液（II液）（即刻组，一周组，两周组），每组10个样本。 其中空白组和各个即刻组，实验即刻观察；各一周组实验浸泡在唾液中置于37度恒温水浴箱内一周后观察，各两周组为实验浸泡在唾液中置于37度恒温水浴箱内两周后观察。全部样本使用扫描电子显微镜观测表面形态，结合能谱分析仪测定牙釉质表面的钙、磷元素含量及钙/磷比值，牙釉质表面使用显微硬度仪测定显微硬度，，所得数据通过SPSS19.0软件进行统计学分析。 结果： 1、扫描电镜观测，低倍镜观察空白组釉质可见正常釉质形态结构，表面较为光滑，高倍镜观察釉柱排列呈鱼鳞状外貌。 实验即刻：Beyond组低倍镜观察釉质表面表面粗糙，高倍镜观察出现明显酸蚀改变，孔隙率增加，釉柱中心及其周围可见明显脱矿溶解，呈现深陷镂空的蚁穴状结构。Beyond+I液组低倍镜观察牙釉质表面表面粗糙比Beyond即刻组严重，高倍镜观察酸蚀明显，正常的釉质表面结构已经消失，代替为明显的空隙和表面凹陷，釉柱中心不明显，呈现密集的空洞表面。低倍镜观察，Beyond+III液组牙釉质粗糙度优于Beyond组，高倍镜观察能够看到空隙率增加，可见到微孔和微融的空洞状釉质表面。 浸泡一周后：低倍镜下观察Beyond组、Beyond+I液组、Beyond+III液组釉质表面粗糙程度减轻，高倍镜观察均有矿物质沉积，牙釉质釉柱中心及其周围可见到部分再矿化，表面空隙率减少，空洞结构密集度降低。Beyond+I液组孔隙结构较Beyond组多，Beyond+III液组孔隙率较低。 浸泡两周后：低倍镜下观察低倍镜下Beyond组、Beyond+I液组、Beyond+III液组釉质表面均较为平坦，矿物质沉积明显，基本恢复釉质正常形态。Beyond+I液组组高倍镜观察牙釉质表面仍可见到部分孔隙结构，但数目明显少于即刻组。Beyond组和Beyond+III液组较少看到孔隙结构。 2、能谱分析结果，即刻组中：Beyond、Beyond+I液显微硬度比值较空白对照组有显著性差异，p0.05，；Beyond+I液与Beyond+III液有显著性差异，p0.05，具有统计学意义。其余组间差异不具有统计学意义。 3、即刻组中：Beyond、Beyond+I液显微硬度比值较空白对照组有显著性差异，p0.05；Beyond、Beyond+III液显微硬度比值较Beyond+I组有显著性差异，p0.05，具有统计学意义。其余组间差异不具有统计学意义。 结论： 1、冷光美白治疗单独使用脱色剂，会加重单纯使用冷光美白剂对釉质的腐蚀，釉质表面粗糙加重，呈现明显的脱矿改变。 2、冷光美白使用脱色剂联合再矿化液，处理后的牙釉质表面物理特性较为稳定，显微硬度无显著性变化。单纯应用冷光美白剂进行临床治疗后，对牙齿进行再矿化治疗，可加速美白后釉质表面钙磷沉积。 3、唾液具有再矿化功能，唾液浸泡保存可使因美白而致的脱矿釉质表面矿物质含量出现上升趋势，可修复因美白所致的釉质表面脱矿。
[Abstract]:Purpose: Through the observation and analysis of the physical properties and the morphological structure of the surface of the permanent enamel treated with the combination of the cold-light whitening and the defluorinating agent and the saliva, the micro-hardness, the composition and the structure of the surface enamel of the healthy permanent tooth were studied, and the shadow of the defluorinating agent and the salivation of the saliva were studied. In response, a theory is provide for that clinical application of a fluorine-removing agent. According to. Real Methods:30 healthy maxillary premolars extracted from the recent orthodontic treatment were selected, and the crown was cut into 4 parts along the proximal and buccal tongue, and 1 was obtained. 20 samples. Select 100 samples of the test plane parallel to the horizontal plane, random divided into 10 groups: blank control group, Beyond whitening gel group (immediate group, one-week group, two-week group), Beyond whitening gel + decolorizer (I-liquid) (immediate group, one-week group, two-week group), Beyond whitening gel + decolorizer (I-liquid) and re-mineralized liquid (II-liquid) (immediate group, one-week group), , two-week group), each group 10 samples. In the blank group and in the immediate group, the experiment was immediately observed; each week group experiment was soaked in saliva to be placed in a 37-degree constant-temperature water bath for one week, and the two-week group was immersed in saliva in a constant-temperature water bath of 37 degrees. After two weeks in the box, the surface morphology was observed with a scanning electron microscope. The calcium, phosphorus content and calcium/ phosphorus ratio of the surface of the enamel were measured by means of the energy spectrum analyzer. The surface of the enamel was measured by a micro-hardness instrument. The obtained data was obtained by the SPSS19.0 software. to be in series The results were as follows:1, the observation of the scanning electron microscope, the observation of the normal enamel structure of the blank group in the blank group, the smooth surface of the surface and the view of high power The results showed that the surface of the enamel surface was rough and the porosity increased, the center of the enamel column and the surrounding of the enamel column were obviously demined and dissolved. In Beyond + I group, the surface roughness of enamel surface is more severe than that of the immediate group of Beyond, and the high-power mirror has obvious acid corrosion. The normal enamel surface structure has disappeared, instead of the obvious void and surface depression, the center of the glaze column It is not obvious that the dense cavity surface is present. The low-magnification observation shows that the enamel roughness of the Beyond + III liquid group is better than that of the Beyond group, and the high-power mirror observation can see the increase of the void ratio, which can be seen. After a week, the surface roughness of the enamel surface of the Beyond group, the Beyond + I liquid group and the Beyond + III liquid group was reduced, and the surface roughness of the enamel surface of the Beyond + I liquid group and the Beyond + III liquid group was reduced. The void ratio is reduced, and the density of the hollow structure is reduced. The pore structure of the Beyond + I liquid group is more than that of the Beyond group, Be The porosity of the yond + III liquid group was lower. After two weeks of soaking, the low-fold microscopic observation of the second group, the Beyond + I liquid group, the Beyond + III liquid group and the enamel surface of the Beyond + III liquid group were relatively flat, In Beyond + I group, the surface of enamel can still be seen. Part of the pore structure, but the number is significantly less than the immediate group. The Beyond group and the Bey The results of spectrum analysis showed that the ratio of the microhardness of Beyond and Beyond + I in the immediate group was significantly different from that of the blank control group. significant difference, p0.05, with system The difference between the other groups was not statistically significant.3. The ratio of the microhardness of the Beyond and the Beyond + I fluid in the immediate group was significantly different from that of the blank control group, and the ratio of the microhardness of the Beyond + III solution to the Beyond + I was higher than that of the Beyond + I. There was a significant difference in the group, p0.05, Statistics The difference between the other groups was not statistically significant. Conclusion:1. The cold-light whitening treatment alone uses the decolorizer, which can increase the pure use of the cold-light beauty. the corrosion of the enamel, the surface roughness of the enamel, the obvious demineralization change,2, the combination of the cold-light whitening and the destaining agent and the mineralizing liquid, The physical properties of the posterior enamel surface were stable and the microhardness was not significantly changed. After the tooth is re-mineralized and treated, the calcium and phosphorus deposition on the surface of the enamel after whitening can be accelerated.3, the saliva has a remineralization function, and the saliva is soaked and stored, so that the demineralization enamel caused by the whitening can be obtained.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783
本文编号：2482508
[Abstract]:Purpose: Through the observation and analysis of the physical properties and the morphological structure of the surface of the permanent enamel treated with the combination of the cold-light whitening and the defluorinating agent and the saliva, the micro-hardness, the composition and the structure of the surface enamel of the healthy permanent tooth were studied, and the shadow of the defluorinating agent and the salivation of the saliva were studied. In response, a theory is provide for that clinical application of a fluorine-removing agent. According to. Real Methods:30 healthy maxillary premolars extracted from the recent orthodontic treatment were selected, and the crown was cut into 4 parts along the proximal and buccal tongue, and 1 was obtained. 20 samples. Select 100 samples of the test plane parallel to the horizontal plane, random divided into 10 groups: blank control group, Beyond whitening gel group (immediate group, one-week group, two-week group), Beyond whitening gel + decolorizer (I-liquid) (immediate group, one-week group, two-week group), Beyond whitening gel + decolorizer (I-liquid) and re-mineralized liquid (II-liquid) (immediate group, one-week group), , two-week group), each group 10 samples. In the blank group and in the immediate group, the experiment was immediately observed; each week group experiment was soaked in saliva to be placed in a 37-degree constant-temperature water bath for one week, and the two-week group was immersed in saliva in a constant-temperature water bath of 37 degrees. After two weeks in the box, the surface morphology was observed with a scanning electron microscope. The calcium, phosphorus content and calcium/ phosphorus ratio of the surface of the enamel were measured by means of the energy spectrum analyzer. The surface of the enamel was measured by a micro-hardness instrument. The obtained data was obtained by the SPSS19.0 software. to be in series The results were as follows:1, the observation of the scanning electron microscope, the observation of the normal enamel structure of the blank group in the blank group, the smooth surface of the surface and the view of high power The results showed that the surface of the enamel surface was rough and the porosity increased, the center of the enamel column and the surrounding of the enamel column were obviously demined and dissolved. In Beyond + I group, the surface roughness of enamel surface is more severe than that of the immediate group of Beyond, and the high-power mirror has obvious acid corrosion. The normal enamel surface structure has disappeared, instead of the obvious void and surface depression, the center of the glaze column It is not obvious that the dense cavity surface is present. The low-magnification observation shows that the enamel roughness of the Beyond + III liquid group is better than that of the Beyond group, and the high-power mirror observation can see the increase of the void ratio, which can be seen. After a week, the surface roughness of the enamel surface of the Beyond group, the Beyond + I liquid group and the Beyond + III liquid group was reduced, and the surface roughness of the enamel surface of the Beyond + I liquid group and the Beyond + III liquid group was reduced. The void ratio is reduced, and the density of the hollow structure is reduced. The pore structure of the Beyond + I liquid group is more than that of the Beyond group, Be The porosity of the yond + III liquid group was lower. After two weeks of soaking, the low-fold microscopic observation of the second group, the Beyond + I liquid group, the Beyond + III liquid group and the enamel surface of the Beyond + III liquid group were relatively flat, In Beyond + I group, the surface of enamel can still be seen. Part of the pore structure, but the number is significantly less than the immediate group. The Beyond group and the Bey The results of spectrum analysis showed that the ratio of the microhardness of Beyond and Beyond + I in the immediate group was significantly different from that of the blank control group. significant difference, p0.05, with system The difference between the other groups was not statistically significant.3. The ratio of the microhardness of the Beyond and the Beyond + I fluid in the immediate group was significantly different from that of the blank control group, and the ratio of the microhardness of the Beyond + III solution to the Beyond + I was higher than that of the Beyond + I. There was a significant difference in the group, p0.05, Statistics The difference between the other groups was not statistically significant. Conclusion:1. The cold-light whitening treatment alone uses the decolorizer, which can increase the pure use of the cold-light beauty. the corrosion of the enamel, the surface roughness of the enamel, the obvious demineralization change,2, the combination of the cold-light whitening and the destaining agent and the mineralizing liquid, The physical properties of the posterior enamel surface were stable and the microhardness was not significantly changed. After the tooth is re-mineralized and treated, the calcium and phosphorus deposition on the surface of the enamel after whitening can be accelerated.3, the saliva has a remineralization function, and the saliva is soaked and stored, so that the demineralization enamel caused by the whitening can be obtained.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783
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