复发性阿弗他溃疡患者可溶性程序性死亡受体1及可溶性程序性死亡配体1表达与免疫功能的相关性
发布时间：2019-05-29 08:41
【摘要】：目的了解复发性阿弗他溃疡(RAU)患者血清中可溶性程序性死亡受体-1(s PD-1)、可溶性程序性死亡配体-1(s PD-L1)蛋白表达与患者免疫功能状态的相关性,探讨s PD-1/s PD-L1在RAU免疫发病机制中的作用及意义。方法研究对象为30例RAU患者(轻型18例,重型5例,疱疹型7例)及18例健康人(对照组)。采用流式细胞术检测RAU患者外周血细胞免疫指标(CD3+、CD4+、CD8+、CD19+、CD16++56+),散射比浊法检测RAU患者体液免疫指标(Ig G、Ig A、Ig M、C3、C4),酶联免疫吸附测定RAU患者及健康人血清中s PD-1、s PD-L1蛋白表达,并对s PD-1、s PD-L1表达水平与RAU患者免疫功能状况及临床特征的相关性进行分析。结果与正常值相比,RAU患者CD4+水平降低,Ig M水平增高,差异有统计学意义(P0.05)。RAU患者的s PD-1及s PD-L1蛋白表达水平高于对照组(P0.05);进一步分析表明,轻型、重型RAU患者的s PD-1及s PD-L1蛋白表达水平高于对照组(P0.05),而疱疹型RAU患者与对照组无统计学差异(P0.05)。RAU患者s PD-1表达量与CD19+、C4存在正相关关系(r1=0.389,P1=0.034;r2=0.382,P2=0.037)。结论 RAU患者以细胞免疫功能低下为主,同时伴有体液免疫功能的紊乱;RAU患者s PD-1、s PD-L1蛋白高表达在一定程度上通过影响PD-1/PD-L1信号通路参与RAU免疫机制的调节,可能在RAU的免疫发病机制中起着一定作用。
[Abstract]:Objective to investigate the relationship between the expression of soluble programmed death receptor-1 (s-PD-1), soluble programmed death ligand-1 (s PD-L1) protein and immune function in patients with recurrent aphthous ulcer (RAU). To explore the role and significance of s PD-1/s PD-L1 in the pathogenesis of RAU immunity. Methods 30 patients with RAU (mild 18 cases, severe 5 cases, herpes type 7 cases) and 18 healthy subjects (control group) were studied. The immune indexes of peripheral blood cells (CD3, CD4, CD8, CD19, CD16 56) and humoral immune indexes (Ig G, IgE, C3, C 4) in patients with RAU were detected by flow cytometry and scattering turbidimetry respectively. The expression of s PD-1,s PD-L1 protein in serum of RAU patients and healthy people was measured by enzyme linked immunosorbent assay (Elisa), and the correlation between the expression of s PD-1,s PD-L1 and immune function and clinical characteristics of RAU patients was analyzed. Results compared with the normal value, the level of CD4 in patients with RAU decreased, Ig M level, the difference was statistically significant (P 0.05). The expression of s PD-1 and s PD-L1 protein in Rau patients was higher than that in the control group (P 0.05). Further analysis showed that the expression of s PD-1 and s PD-L1 protein in patients with mild and severe RAU was higher than that in the control group (P 0.05). There was no significant difference between the patients with herpes RAU and the control group (P 0.05). There was a positive correlation between the expression of s PD-1 and CD19 and C 4 in Rau patients (R1 鈮，
本文编号：2487792
[Abstract]:Objective to investigate the relationship between the expression of soluble programmed death receptor-1 (s-PD-1), soluble programmed death ligand-1 (s PD-L1) protein and immune function in patients with recurrent aphthous ulcer (RAU). To explore the role and significance of s PD-1/s PD-L1 in the pathogenesis of RAU immunity. Methods 30 patients with RAU (mild 18 cases, severe 5 cases, herpes type 7 cases) and 18 healthy subjects (control group) were studied. The immune indexes of peripheral blood cells (CD3, CD4, CD8, CD19, CD16 56) and humoral immune indexes (Ig G, IgE, C3, C 4) in patients with RAU were detected by flow cytometry and scattering turbidimetry respectively. The expression of s PD-1,s PD-L1 protein in serum of RAU patients and healthy people was measured by enzyme linked immunosorbent assay (Elisa), and the correlation between the expression of s PD-1,s PD-L1 and immune function and clinical characteristics of RAU patients was analyzed. Results compared with the normal value, the level of CD4 in patients with RAU decreased, Ig M level, the difference was statistically significant (P 0.05). The expression of s PD-1 and s PD-L1 protein in Rau patients was higher than that in the control group (P 0.05). Further analysis showed that the expression of s PD-1 and s PD-L1 protein in patients with mild and severe RAU was higher than that in the control group (P 0.05). There was no significant difference between the patients with herpes RAU and the control group (P 0.05). There was a positive correlation between the expression of s PD-1 and CD19 and C 4 in Rau patients (R1 鈮，
本文编号：2487792
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