局部注射黄芪多糖对大鼠正畸牙保持阶段BMP-2表达的影响
发布时间：2019-05-30 02:40
【摘要】：目的:探讨局部注射黄芪多糖对大鼠正畸牙保持阶段牙周组织改建和骨形成蛋白2(BMP-2)表达的影响。方法:48只8周龄雄性Wistar大鼠随机分为8组,每组6只,即空白对照组(1组),阴性对照组(1组),实验组(6组)。利用50g牵引力近中移动右侧上颌第一磨牙,建立大鼠正畸牙移动模型,加力21d。实验组于拆除装置前1d开始将10mg/L黄芪多糖溶液局部注射于右上第一磨牙远中颊侧黏骨膜下,每1次/3d,每次50μL;阴性对照组每3d注射等量生理盐水。分别于1、3、7、14、21、28d后处死,测量正畸牙复发距离,采用HE染色观察牙周组织变化,免疫组化染色检测牙周组织中BMP-2的表达。结果:实验组牙周膜宽度保持较好,除1d组外,相同时间的实验组复发距离小于对照组,差异有统计学意义(P0.05)。在整个保持阶段,对照组远中侧BMP-2表达强度逐渐减少,实验组远中侧BMP-2表达强度逐渐增大。除1d组外,相同时间的实验组BMP-2表达强度显著高于对照组,差异有统计学意义(P0.05)。结论:大鼠正畸牙保持阶段,局部注射黄芪多糖能够增加牙周组织中BMP-2的表达,从而加速牙槽骨的改建,有利于正畸后牙齿的保持。
[Abstract]:Aim: to investigate the effect of local injection of astragalus polysaccharide on periodontal tissue remodeling and bone morphogenetic protein 2 (BMP-2) expression during orthodontic tooth retention in rats. Methods: 48 8-week-old male Wistar rats were randomly divided into 8 groups with 6 rats in each group, namely, blank control group (group 1), negative control group (group 1) and experimental group (group 6). The orthodontic tooth movement model of rats was established by using 50g traction force to move the right maxillary first molar near the middle, and the force was increased for 21 days. The experimental group began to inject 10mg/L astragalus polysaccharide solution into the distal buccal mucoperiosteum of the right upper first molar one day before the device was removed, each time every 3 days, 50 渭 L each time, and the negative control group was injected with the same amount of normal saline every 3 days. After 1, 3, 7, 14, 21, 28 days, the recurrence distance of orthodontic teeth was measured, the changes of periodontal tissue were observed by HE staining, and the expression of BMP-2 in periodontal tissue was detected by immunohistochemical staining. Results: the width of periodontal ligament in the experimental group was better than that in the control group, except for 1 day group, the recurrence distance of the experimental group was smaller than that of the control group at the same time, and the difference was statistically significant (P 0.05). During the whole retention stage, the expression intensity of BMP-2 in the control group decreased gradually, while the expression intensity of BMP-2 in the experimental group increased gradually. Except for 1 day group, the expression intensity of BMP-2 in the experimental group was significantly higher than that in the control group at the same time, the difference was statistically significant (P 0.05). Conclusion: in orthodontic tooth retention stage, local injection of astragalus polysaccharide can increase the expression of BMP-2 in periodontal tissue, thus accelerate alveolar bone remodeling, which is beneficial to tooth maintenance after orthodontic treatment.
【作者单位】： 济南市口腔医院正畸科;
【基金】：山东省医药卫生科技发展计划项目(编号:2016WS0121) 济南市科技卫生计划项目(编号:2013-65)
【分类号】：R783.5
本文编号：2488445
[Abstract]:Aim: to investigate the effect of local injection of astragalus polysaccharide on periodontal tissue remodeling and bone morphogenetic protein 2 (BMP-2) expression during orthodontic tooth retention in rats. Methods: 48 8-week-old male Wistar rats were randomly divided into 8 groups with 6 rats in each group, namely, blank control group (group 1), negative control group (group 1) and experimental group (group 6). The orthodontic tooth movement model of rats was established by using 50g traction force to move the right maxillary first molar near the middle, and the force was increased for 21 days. The experimental group began to inject 10mg/L astragalus polysaccharide solution into the distal buccal mucoperiosteum of the right upper first molar one day before the device was removed, each time every 3 days, 50 渭 L each time, and the negative control group was injected with the same amount of normal saline every 3 days. After 1, 3, 7, 14, 21, 28 days, the recurrence distance of orthodontic teeth was measured, the changes of periodontal tissue were observed by HE staining, and the expression of BMP-2 in periodontal tissue was detected by immunohistochemical staining. Results: the width of periodontal ligament in the experimental group was better than that in the control group, except for 1 day group, the recurrence distance of the experimental group was smaller than that of the control group at the same time, and the difference was statistically significant (P 0.05). During the whole retention stage, the expression intensity of BMP-2 in the control group decreased gradually, while the expression intensity of BMP-2 in the experimental group increased gradually. Except for 1 day group, the expression intensity of BMP-2 in the experimental group was significantly higher than that in the control group at the same time, the difference was statistically significant (P 0.05). Conclusion: in orthodontic tooth retention stage, local injection of astragalus polysaccharide can increase the expression of BMP-2 in periodontal tissue, thus accelerate alveolar bone remodeling, which is beneficial to tooth maintenance after orthodontic treatment.
【作者单位】： 济南市口腔医院正畸科;
【基金】：山东省医药卫生科技发展计划项目(编号:2016WS0121) 济南市科技卫生计划项目(编号:2013-65)
【分类号】：R783.5
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，本文编号：2488445
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