肿瘤坏死因子样配体1A和吲哚胺2，3双加氧酶在OLP患者PBMCs中的表达

发布时间：2019-06-05 06:04

【摘要】：目的:口腔扁平苔藓（oral lichen planus，，OLP），是口腔黏膜的慢性非感染性疾病，是一种较为常见的口腔黏膜疾病，其发病仅低于复发性口腔溃疡，病情反复发作，可伴充血糜烂，难以治愈，属于癌前状态。其病因尚不明确，有研究显示，OLP是一种由T淋巴细胞介导的自身免疫反应性疾病。 本研究通过检测肿瘤坏死因子样配体1A（tumor necrosis factor-like ligand1A，TL1A）和吲哚胺2，3双加氧酶（indoleamine2，3-dioxygenase，IDO）在健康人、口腔扁平苔藓患者外周血单个核细胞中的表达水平，探讨两者分别在口腔扁平苔藓中的表达及意义，推测这两个因子在扁平苔藓发病机制中的作用。 方法:采用实时荧光定量聚合酶链式反应（Real-time fluorescent quantitative PCR，FQ-PCR）法检测30例OLP患者和30例健康者外周血单个核细胞（peripheral bloodmononuclear cell，PBMCs）中TL1A、IDO mRNA的相对表达量，选β肌动蛋白（β-actin）为内参照基因，FQ-PCR中采用2-△△CT方法，检测TL1A、IDO mRNA的相对表达量。 结果:1.在口腔扁平苔藓患者外周血单个核细胞中，TL1A的基因表达水平均高于健康对照组，经统计学分析，有显著性差异(P 0.05)。口腔扁平苔藓患者组中TL1A的基因表达量是正常对照组的1.7872倍。 2．在口腔扁平苔藓患者外周血单个核细胞中，IDO的基因表达水平均高于健康对照组，经统计学分析，有显著性差异(P 0.05)。口腔扁平苔藓患者组IDO的基因表达水平是正常对照组的2.0763倍。 3.在口腔扁平苔藓患者组，TL1A和IDO基因表达量经Pearson线性相关分析，相关系数r=0.124，(P=0.5150.05)，表明两组数据无相关性。 结论:1. TL1AmRNA在OLP患者组中的表达水平明显高于正常对照组，表明TL1A可能参与OLP的发病过程。 2. IDOmRNA在OLP患者组中的表达水平明显高于正常对照组，表明IDO可能参与OLP炎症的发生发展，其机制有待于进一步研究。 3.在OLP患者组TL1A和IDO基因表达无直接相关性，TL1A和IDO也有可能通过间接途径或方式发挥着作用。
[Abstract]:Objective: oral lichen planus (oral lichen planus,OLP) is a chronic non-infectious disease of oral mucous membrane. It is a common oral mucous membrane disease, which is only lower than that of recurrent oral ulcer and can be accompanied by hyperemia and erosion. Difficult to cure, belonging to the precancerous state. Its etiology is not clear, some studies have shown that OLP is an autoimmune disease mediated by T lymphocytes. In this study, the expression of tumor necrosis factor-like ligand 1A (tumor necrosis factor-like ligand1A,TL1A and indoleamine 2, 3 dioxygenase (indoleamine2,3-dioxygenase,IDO) in peripheral blood mononuclear cells of healthy people and patients with oral lichen planus was detected. To explore the expression and significance of the two factors in oral lichen planus, and to speculate the role of these two factors in the pathogenesis of oral lichen planus. Methods: the relative expression of TL1A,IDO mRNA in peripheral blood mononuclear cells (peripheral bloodmononuclear cell,PBMCs) of 30 patients with OLP and 30 healthy controls was detected by real-time fluorescence quantitative polymerase chain reaction (Real-time fluorescent quantitative PCR,FQ-PCR). 尾-actin (尾-actin) was selected as internal reference gene. 2-CT method was used to detect the relative expression of TL1A,IDO mRNA in FQ-PCR. Result: 1. The gene expression level of TL1A in peripheral blood mononuclear cells of patients with oral lichen planus was higher than that of healthy control group, and there was significant difference between the two groups (P 0.05). The expression of TL1A gene in oral lichen planus group was 1.7872 times higher than that in normal control group. 2. The gene expression level of IDO in peripheral blood mononuclear cells of patients with oral lichen planus was higher than that of healthy control group, and there was significant difference between the two groups (P 0.05). The gene expression level of IDO in oral lichen planus group was 2.0763 times higher than that in normal control group. 3. In the oral lichen planus group, the expression of TL1A and IDO genes was analyzed by Pearson linear correlation analysis, and the correlation coefficient was r 鈮

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