聚酰胺—胺及其纳米复合生物材料诱导牙本质小管的仿生再矿化研究
发布时间：2019-06-15 12:17
【摘要】：目的:实验一研究3.5代羧基改性后的聚酰胺-胺树枝状高分子聚合物及其诱导人类脱矿牙本质仿生再矿化作用。不同浓度的PAMAM溶液诱导脱矿牙本质仿生再矿化的能力是否有差异为本实验的主要研究侧重点。实验二合成并表征同时具有有机、无机材料特性的纳米复合生物材料聚酰胺-胺/纳米羟磷灰石(PAMAM/n-HAP),并观察其对牙本质小管的封闭作用。方法:实验一通过口腔颌面外科收集40颗完整离体第三磨牙,并将其相应制备成脱矿牙本质模型(每颗牙齿制备一个模型)。随后随机分为A、B、C、D4组,分别接受不同浓度3.5代羧基改性的聚酰胺-胺聚合物(PAMAM-COOH)处理,后浸泡于人工唾液接受再矿化处理14d,后使用傅里叶红外变换光谱(FTIR)检测PAMAM-COOH分子与牙本质表面的结合情况;牙本质小管的微观形貌通过扫描电子显微镜(SEM)表征。实验二通过实验室制备PAMAM/n-HAP纳米复合生物材料(采用Pramanik水化学反应的方法)。使用透射电子显微镜(TEM)、傅里叶变换红外光谱仪(FTIR)对纳米复合生物材料进行表征和验证。将脱矿牙本质模型随机分为A、B、C、D四组,分别采用等量的去离子水、PAMAM/n-HAP、n-HAP、PAMAM/n-HAP处理,A、B、C三组浸泡于人工唾液7d接受再矿化处理,D组浸泡于去离子水中7d。使用扫描电子显微镜(SEM)对其牙本质小管的修复封闭效果进行检测。并采用显微硬度仪对其机械性能的恢复进行检测和比较。结果:实验一:FTIR结果显示G3.5 PAMAM-COOH能够牢固结合于脱矿牙本质模型的表面,并且高浓度PAMAM-COOH处理后的牙本质磨片显示出更强的特征峰值。SEM结果显示应用不同浓度的PAMAM-COOH虽然能够在一定程度诱导新生的矿物晶体沉积封闭牙本质小管,然而均不能够高效的封闭暴露的牙本质小管。SEM的结果显示,不同浓度PAMAM-COOH所诱导的实验组未见明显的封闭差异。实验二:FTIR显示1244 cm-1和1650 cm-1出现了PAMAM所特有的强振动峰,同时TEM显示纳米羟磷灰石的表面出现10nm厚的低衬度包裹层,证实了PAMAM/n-HAP的成功合成。SEM显示应用PAMAM/n-HAP能够有效的降低牙本质小管的直径,修复并填充空虚的牙本质小管,封闭牙本质小管,从而获得良好的封闭作用。结论:1.G3.5 PAMAM-COOH能够吸附并稳定于纳米羟磷灰石的表面。2.在某种程度上,G3.5 PAMAM-COOH能够诱导矿化沉积物沉积于牙本质磨片表面或者小管内部,但是晶体外形不规则,矿化沉积物量较少,封闭效果不理想,并且本实验的扫描电镜结果显示不同浓度的G3.5PAMAM-COOH(1000ppm,5000ppm,以及10000ppm)其诱导牙本质小管封闭的效果并没有明显的差异。3.作为一种新型的纳米复合生物材料,人工合成的PAMAM/n-HAP具有良好的生物活性,能够有效地封闭牙本质小管,同时有较为理想的封闭深度,提示PAMAM/n-HAP可能成为一种新型的脱敏材料或者脱敏药物添加剂应用于临床。4.PAMAM/n-HAP通过修复脱矿牙本质小管,能够恢复其一定的硬度,说明其在一定程度能够恢复机械性能。
[Abstract]:Objective: To study the biomimetic re-mineralization of polyetheramine-amine dendrimer and its induced demineralization of human demineralization. The ability of the different concentration of the PAMAM solution to induce the biomimetic re-mineralization of the demineralization dentin is different from the main research focus of the experiment. The composite biological materials with organic and inorganic materials (PAMAM/ n-HAP) were synthesized and characterized at the same time, and its effect on the dentinal tubules was observed. Methods:40 intact third molars were collected by oral and maxillofacial surgery and prepared into a demineralization dentin model (a model for each tooth). then the groups are randomly divided into groups A, B, C and D4, and then treated by a polycarbodiimide-amine polymer (PAMAM-COOH) with different concentration of 3.5 generations, and then is soaked in artificial saliva to be subjected to the re-mineralization treatment 14d, The binding of PAMAM-COOH and the surface of dentin was detected by FTIR. The micromorphology of the dentinal tubules was characterized by scanning electron microscopy (SEM). The preparation of PAMAM/ n-HAP nano-composite biomaterial by the laboratory was carried out by a laboratory. The composite biological materials were characterized and verified by a transmission electron microscope (TEM) and a Fourier transform infrared spectrometer (FTIR). The demineralization dentin model was randomly divided into four groups: A, B, C and D, and the same amount of deionized water, PAMAM/ n-HAP, n-HAP, PAMAM/ n-HAP were used, and the three groups of A, B and C were soaked in artificial saliva for 7 days to be re-mineralized, and the D group was soaked in deionized water for 7 days. A scanning electron microscope (SEM) was used to test the effect of the repair and closure of the dentinal tubules. And the mechanical property recovery of the device is detected and compared by a micro-hardness meter. Results: The results showed that the results showed that the results of FTIR showed that the G3. 5PAMAM-COOH can be firmly combined with the surface of the demineralization dentine model, and the high-concentration PAMAM-COOH treated dentin grinding disc shows a stronger characteristic peak. SEM results show that the application of PAMAM-COOH at different concentrations can induce new mineral crystals to close the dentinal tubules to a certain extent, but can not close the exposed dentinal tubules efficiently. The results of SEM showed that there was no significant difference in the experimental group induced by different concentration of PAMAM-COOH. Experiment 2: FTIR showed that 1244 cm-1 and 1650 cm-1 showed a strong vibration peak unique to the PAMAM, while the TEM showed that the surface of the nano-hydroxyapatite was 10 nm thick low-contrast coating layer, which confirmed the successful synthesis of the PAMAM/ n-HAP. SEM showed that the application of PAMAM/ n-HAP can effectively reduce the diameter of the dentinal tubules, repair and fill the empty dentin tubules and close the dentinal tubules, thus obtaining a good sealing effect. Conclusion:1. G3. 5PAMAM-COOH can adsorb and stabilize the surface of the nano-hydroxyapatite. In some ways, the G3. 5PMAM-COOH can induce the deposit of the mineralized deposit on the surface of the dentin grinding disc or the inside of the tubule, but the crystal is irregular, the amount of the mineralized deposit is small, the sealing effect is not ideal, and the scanning electron microscope result of the experiment shows that the G3. 5PAMAM-COOH (1000 ppm,5000 ppm, And there was no significant difference in the effect of the induction of the closure of the dentinal tubules. As a novel nano composite biological material, the artificially synthesized PAMAM/ n-HAP has good biological activity, can effectively close the dentinal tubules, and has an ideal sealing depth, It is suggested that the PAMAM/ n-HAP may be a new desensitizing material or desensitizing drug additive.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781.2
，
本文编号：2500201
[Abstract]:Objective: To study the biomimetic re-mineralization of polyetheramine-amine dendrimer and its induced demineralization of human demineralization. The ability of the different concentration of the PAMAM solution to induce the biomimetic re-mineralization of the demineralization dentin is different from the main research focus of the experiment. The composite biological materials with organic and inorganic materials (PAMAM/ n-HAP) were synthesized and characterized at the same time, and its effect on the dentinal tubules was observed. Methods:40 intact third molars were collected by oral and maxillofacial surgery and prepared into a demineralization dentin model (a model for each tooth). then the groups are randomly divided into groups A, B, C and D4, and then treated by a polycarbodiimide-amine polymer (PAMAM-COOH) with different concentration of 3.5 generations, and then is soaked in artificial saliva to be subjected to the re-mineralization treatment 14d, The binding of PAMAM-COOH and the surface of dentin was detected by FTIR. The micromorphology of the dentinal tubules was characterized by scanning electron microscopy (SEM). The preparation of PAMAM/ n-HAP nano-composite biomaterial by the laboratory was carried out by a laboratory. The composite biological materials were characterized and verified by a transmission electron microscope (TEM) and a Fourier transform infrared spectrometer (FTIR). The demineralization dentin model was randomly divided into four groups: A, B, C and D, and the same amount of deionized water, PAMAM/ n-HAP, n-HAP, PAMAM/ n-HAP were used, and the three groups of A, B and C were soaked in artificial saliva for 7 days to be re-mineralized, and the D group was soaked in deionized water for 7 days. A scanning electron microscope (SEM) was used to test the effect of the repair and closure of the dentinal tubules. And the mechanical property recovery of the device is detected and compared by a micro-hardness meter. Results: The results showed that the results showed that the results of FTIR showed that the G3. 5PAMAM-COOH can be firmly combined with the surface of the demineralization dentine model, and the high-concentration PAMAM-COOH treated dentin grinding disc shows a stronger characteristic peak. SEM results show that the application of PAMAM-COOH at different concentrations can induce new mineral crystals to close the dentinal tubules to a certain extent, but can not close the exposed dentinal tubules efficiently. The results of SEM showed that there was no significant difference in the experimental group induced by different concentration of PAMAM-COOH. Experiment 2: FTIR showed that 1244 cm-1 and 1650 cm-1 showed a strong vibration peak unique to the PAMAM, while the TEM showed that the surface of the nano-hydroxyapatite was 10 nm thick low-contrast coating layer, which confirmed the successful synthesis of the PAMAM/ n-HAP. SEM showed that the application of PAMAM/ n-HAP can effectively reduce the diameter of the dentinal tubules, repair and fill the empty dentin tubules and close the dentinal tubules, thus obtaining a good sealing effect. Conclusion:1. G3. 5PAMAM-COOH can adsorb and stabilize the surface of the nano-hydroxyapatite. In some ways, the G3. 5PMAM-COOH can induce the deposit of the mineralized deposit on the surface of the dentin grinding disc or the inside of the tubule, but the crystal is irregular, the amount of the mineralized deposit is small, the sealing effect is not ideal, and the scanning electron microscope result of the experiment shows that the G3. 5PAMAM-COOH (1000 ppm,5000 ppm, And there was no significant difference in the effect of the induction of the closure of the dentinal tubules. As a novel nano composite biological material, the artificially synthesized PAMAM/ n-HAP has good biological activity, can effectively close the dentinal tubules, and has an ideal sealing depth, It is suggested that the PAMAM/ n-HAP may be a new desensitizing material or desensitizing drug additive.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781.2
，
本文编号：2500201
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