免疫抑制剂MT01对正畸牙移动过程中牙周组织TLR9、TRAF6和IL-6表达的影响
发布时间：2019-06-15 19:50
【摘要】：正畸牙移动是压力侧骨吸收伴随张力侧骨形成的骨改建过程,许多免疫介质如炎症因子、生长因子等都参与正畸牙移动过程。因此牙齿移动被认为属于无菌炎症反应过程。MT01属免疫抑制型ODN,前期研究表明,MT01能够抑制由TLR9激活引起的机体炎症反应,防止组织细胞受损,并能够促进牙移动过程中牙槽骨的成骨细胞分化,抑制破骨细胞增值活化。那么,TLR9是否参与引发正畸牙移动牙槽骨炎症反应的过程,MT01又是否能够通过抑制TLR9信号通路,从而抑制牙移动过程中的炎症反应。根据所提出的科学问题,本实验将探讨MT01对正畸牙移动中牙周组织免疫反应的调控作用及可能的机制。目的:建立大鼠正畸牙移动模型,局部途径给药,通过RT-q PCR方法检测探讨TLR9、TRAF6和其下游炎症因子IL-6在实验性牙移动过程中牙周组织表达水平的变化,以及MT01对牙周组织TLR9、TRAF6和IL-6表达水平的影响,评价MT01对大鼠正畸牙移动后牙周组织改建的影响作用。方法:72只雄性Wistar大鼠,随机分为无药物干预组(n=36)及药物干预组(n=36),0.49 N力近中移动大鼠上颌第一磨牙,于加力后第3、7、14、21天处死。其中,每组6只断头处死后分别获取上颌第一磨牙近远中侧牙槽骨,行实时定量荧光PCR检测TLR9、TRAF6和IL-6m RNA的相对表达量;其余每组3只于心脏灌注处死后取上颌第一磨牙及其周围牙周组织制作石蜡切片,经HE染色观察牙周组织形态变化。结果:①无MT01干预下,加力组张力侧TLR9、TRAF6和IL-6m RNA各时间点表达量均显著高于对照组(P0.01),压力侧加力7、14、21天时TLR9、TRAF6和IL-6m RNA表达量均显著高于对照组(P0.01),且压力组及张力组各个因子相对表达量于第7天达高峰;②MT01干预下,给药组张力侧加力3天时TLR9、TRAF6和IL-6相对表达量与对照组无统计学差异,加力14、21天时TLR9和IL-6表达量均低于对照组(P0.01),TRAF6表达量与对照组无显著差异,压力侧加力3天时TLR9和IL-6相对表达量即显著降低,加力21天时TLR9、TRAF6和IL-6三者表达量均显著低于对照组(P0.01);③HE染色观察显示,给药组大鼠第一磨牙压力侧牙槽骨吸收程度较对照组轻,且张力侧成骨较对照组活跃;结论:实验性牙移动牙周组织中TLR9、TRAF6及其下游炎症因子IL-6的表达升高;MT01能够抑制正畸牙移动过程中牙周组织TLR9及下游相关因子表达。
[Abstract]:Orthodontic tooth movement is a process of bone remodeling accompanied by tension bone resorption. Many immune mediators, such as inflammatory factors and growth factors, are involved in orthodontic tooth movement. Therefore, tooth movement is considered to belong to aseptic inflammatory reaction. MT01 belongs to immunosuppressive ODN,. Previous studies show that MT01 can inhibit the inflammatory reaction caused by TLR9 activation, prevent tissue cell damage, and promote the differentiation of alveolar bone osteoblasts and inhibit the increment and activation of osteoclasts during tooth movement. Then, whether TLR9 is involved in the process of alveolar bone inflammation induced by orthodontic tooth movement, and whether MT01 can inhibit the inflammatory response in the process of tooth movement by inhibiting TLR9 signal pathway. According to the scientific questions put forward, this experiment will explore the regulatory effect of MT01 on periodontal tissue immune response in orthodontic tooth movement and its possible mechanism. Aim: to establish a rat orthodontic tooth movement model and local administration. To investigate the expression of TLR9,TRAF6 and its downstream inflammatory factor IL-6 in periodontal tissue during experimental tooth movement by RT-q PCR, and the effect of MT01 on the expression of TLR9,TRAF6 and IL-6 in periodontal tissue, and to evaluate the effect of MT01 on periodontal tissue remodeling after orthodontic tooth movement in rats. Methods: seventy-two male Wistar rats were randomly divided into two groups: no drug intervention group (n 鈮，
本文编号：2500467
[Abstract]:Orthodontic tooth movement is a process of bone remodeling accompanied by tension bone resorption. Many immune mediators, such as inflammatory factors and growth factors, are involved in orthodontic tooth movement. Therefore, tooth movement is considered to belong to aseptic inflammatory reaction. MT01 belongs to immunosuppressive ODN,. Previous studies show that MT01 can inhibit the inflammatory reaction caused by TLR9 activation, prevent tissue cell damage, and promote the differentiation of alveolar bone osteoblasts and inhibit the increment and activation of osteoclasts during tooth movement. Then, whether TLR9 is involved in the process of alveolar bone inflammation induced by orthodontic tooth movement, and whether MT01 can inhibit the inflammatory response in the process of tooth movement by inhibiting TLR9 signal pathway. According to the scientific questions put forward, this experiment will explore the regulatory effect of MT01 on periodontal tissue immune response in orthodontic tooth movement and its possible mechanism. Aim: to establish a rat orthodontic tooth movement model and local administration. To investigate the expression of TLR9,TRAF6 and its downstream inflammatory factor IL-6 in periodontal tissue during experimental tooth movement by RT-q PCR, and the effect of MT01 on the expression of TLR9,TRAF6 and IL-6 in periodontal tissue, and to evaluate the effect of MT01 on periodontal tissue remodeling after orthodontic tooth movement in rats. Methods: seventy-two male Wistar rats were randomly divided into two groups: no drug intervention group (n 鈮，
本文编号：2500467
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